Aluminum subcellular distribution and its combining characreristics with cell wall in tea leaves

doi:10.3969/j.issn.1004-1524.2023.03.03

Acta Agriculturae Zhejiangensis ›› 2023, Vol. 35 ›› Issue (3): 509-514.DOI: 10.3969/j.issn.1004-1524.2023.03.03

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Aluminum subcellular distribution and its combining characreristics with cell wall in tea leaves

LI Chunlei¹(), XU Hongmei¹, LIU Jie¹, ZHANG Rujun¹, MA Xingyun¹, ZHANG Hua²

1. Shandong Facility Horticulture Bioengineering Research Center, College of Agronomy, Weifang University of Science and Technology, Shouguang 262700, Shandong, China
2. College of Horticultural Science and Engineering, Shandong Agricultural University,Tai’an 271018, Shandong, China

Received:2022-04-29 Online:2023-03-25 Published:2023-04-07

Abstract

Abstract:

Tea tree (Camellia sinensis) is a very typical aluminium (Al) concentrating plant, which is mainly enriched in the leaves. In order to analyze the characteristics of Al content in tea leaves, this paper explored the accumulation characteristics of Al in tea leaves using mature leaves of the high-Al variety Nongkangzao and the low-Al variety Pingyangtezao as materials, and analyzed the subcellular distribution of Al in tea leaves and the possible binding mode with the cell wall. The results showed that Al in the leaves of the two varieties (Nongkangzao and Pingyangtezao) was mainly distributed in the cell wall (46.2%-54.9%), and to a less extent in the nucleus and chloroplasts (24.2%-31.2%). In the cell wall fractions, pectin was the main Al-enriched component of the cell wall, accounting for 60.1%-79.0% of the Al content in the cell wall. Both methyl-modified and esterified treatments significantly reduced the Al content of the cell wall, while the cellulase and pectinase enzymatic treatments had less effect on the Al content of the cell wall, suggesting that the -COOH and -NH₂ in the cell wall play a key role in binding Al, while the length of the sugar molecule chains had no significant effect on the Al binding. Therefore, the cell wall of tea leaves is the main location where Al accumulates, preventing it from entering further into the cell interior and reducing its toxic effects; Al is most probably bound to the carboxyl and amino groups in the cell wall in the form of a hydrogen bond and is immobilised in the cell wall.

Key words: Camellia sinensis, aluminum, cell wall, subcellular distribution, combining characteristics

CLC Number:

S571.1

LI Chunlei, XU Hongmei, LIU Jie, ZHANG Rujun, MA Xingyun, ZHANG Hua. Aluminum subcellular distribution and its combining characreristics with cell wall in tea leaves[J]. Acta Agriculturae Zhejiangensis, 2023, 35(3): 509-514.

Figures/Tables 7

Fig.1 Subcellular distribution of Al in tea plant leaves Different lowercase letters in the same cultivar indicate a significant difference at P<0.05. The same as below.

Fig.2 Relative proportion of Al subcellular distribution in tea plant leaves of Pingyangtezao

Fig.3 Relative proportion of Al subcellular distribution in tea plant leaves of Nongkangzao

Fig.4 Al distribution in cell wall fractions in tea plant leaves

Fig.5 Relative Al percentages in cell wall fractions of Pingyangtezao

Fig.6 Relative percentages of Al in cell wall fractions of Nongkangzao

Fig.7 The Al content of cell wall with methlation modification Different lowercase letters between the same cultivar and the same treatment indicate a significant difference at P<0.05.

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Aluminum subcellular distribution and its combining characreristics with cell wall in tea leaves

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