HPLC fingerprint of cultivated Sanghuangporus and content determination of main component

doi:10.3969/j.issn.1004-1524.2023.03.19

Acta Agriculturae Zhejiangensis ›› 2023, Vol. 35 ›› Issue (3): 658-665.DOI: 10.3969/j.issn.1004-1524.2023.03.19

• Food Science • Previous Articles Next Articles

HPLC fingerprint of cultivated Sanghuangporus and content determination of main component

ZHONG Shi¹(), CAO Yinglong²^,³, WANG Wenqiong²^,³, HUO Jinxi¹, SUN Yuqing¹, XUAN Lijiang²^,³, LI Yougui¹^,^*()

1. Institute of Sericulture and Tea, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China
2. State Key Laboratory of Drug Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 202009, China
3. University of Chinese Academy of Sciences, Beijing 100049, China

Received:2021-09-30 Online:2023-03-25 Published:2023-04-07

Abstract

Abstract:

To establish HPLC fingerprint of cultivated Sanghuangporus(Sanghuang), screen out the signature component of Sanghuangporus through chemical pattern recognition, the content determination method of Sanghuangporus was established based on this index. According to the corresponding methods in the general rules of The Chinese Pharmacopoeia (four parts) in the 2020 edition, the fingerprint of Sanghuangporus was established, and the similarity was evaluated by using the similarity evaluation system of traditional Chinese Medicine Chromatographic Fingerprint 2004 Version A to determine the common peak. The content of Hypholomine B in Sanghuangporus was also determined. HPLC fingerprints of 10 batches of Sanghuangporus were established, with 12 common peaks, and the similarity was greater than 0.92. The results of HCA, PCA and OPLS-DA were consistent, and the samples were divided into two categories. The injection volume of Hypholomine B had a good linear range with its peak area in the range of 1.25-20.00 μg (R²=0.999 9). The RSD values of precision, stability and repeatability were all less than 2.0% (n=6). The recovery rate was 95%-99%, and the RSD was 1.48%. The content of Hypholomine B in 10 batches of mulberry yellow samples ranged from 0.15% to 0.95%. The fingerprint can be combined with HCA, PCA and OPLS-DA to comprehensively evaluate the quality of Sanghuangporus. The method is stable and reliable, providing reference for the quality evaluation of Sanghuangporus.

Key words: Sanghuangporus, fingerprints, high performance liquid chromatography, styrylpyranones, Hypholomine B

CLC Number:

S888

ZHONG Shi, CAO Yinglong, WANG Wenqiong, HUO Jinxi, SUN Yuqing, XUAN Lijiang, LI Yougui. HPLC fingerprint of cultivated Sanghuangporus and content determination of main component[J]. Acta Agriculturae Zhejiangensis, 2023, 35(3): 658-665.

Figures/Tables 12

References 18

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编号 No.			栽培基质 Culture medium
S1	A1	浙江千济方医药科技公司Zhejiang Qianjifang Pharmaceutical Technology Co., Ltd.	椴木Basswood
S2	A2	浙江千济方医药科技公司Zhejiang Qianjifang Pharmaceutical Technology Co., Ltd.	椴木Basswood
S3	B1	海宁宏欣农业生物科技公司Haining Hongxin Agricultural Biotechnology Co., Ltd.	人工代料Manual substitution
S4	B2	海宁宏欣农业生物科技公司Haining Hongxin Agricultural Biotechnology Co., Ltd.	人工代料Manual substitution
S5	B3	海宁宏欣农业生物科技公司Haining Hongxin Agricultural Biotechnology Co., Ltd.	人工代料Manual substitution
S6	C1	淳安千岛湖桑都食用菌合作社Chun’an Qiandao Lake Sangdu Edible Fungus Cooperative	人工代料Manual substitution
S7	C2	淳安千岛湖桑都食用菌合作社Chun’an Qiandao Lake Sangdu Edible Fungus Cooperative	人工代料Manual substitution
S8	C3	淳安千岛湖桑都食用菌合作社Chun’an Qiandao Lake Sangdu Edible Fungus Cooperative	人工代料Manual substitution
S9	C4	淳安千岛湖桑都食用菌合作社Chun’an Qiandao Lake Sangdu Edible Fungus Cooperative	人工代料Manual substitution
S10	C5	淳安千岛湖桑都食用菌合作社Chun’an Qiandao Lake Sangdu Edible Fungus Cooperative	人工代料Manual substitution

编号 No.			栽培基质 Culture medium
S1	A1	浙江千济方医药科技公司Zhejiang Qianjifang Pharmaceutical Technology Co., Ltd.	椴木Basswood
S2	A2	浙江千济方医药科技公司Zhejiang Qianjifang Pharmaceutical Technology Co., Ltd.	椴木Basswood
S3	B1	海宁宏欣农业生物科技公司Haining Hongxin Agricultural Biotechnology Co., Ltd.	人工代料Manual substitution
S4	B2	海宁宏欣农业生物科技公司Haining Hongxin Agricultural Biotechnology Co., Ltd.	人工代料Manual substitution
S5	B3	海宁宏欣农业生物科技公司Haining Hongxin Agricultural Biotechnology Co., Ltd.	人工代料Manual substitution
S6	C1	淳安千岛湖桑都食用菌合作社Chun’an Qiandao Lake Sangdu Edible Fungus Cooperative	人工代料Manual substitution
S7	C2	淳安千岛湖桑都食用菌合作社Chun’an Qiandao Lake Sangdu Edible Fungus Cooperative	人工代料Manual substitution
S8	C3	淳安千岛湖桑都食用菌合作社Chun’an Qiandao Lake Sangdu Edible Fungus Cooperative	人工代料Manual substitution
S9	C4	淳安千岛湖桑都食用菌合作社Chun’an Qiandao Lake Sangdu Edible Fungus Cooperative	人工代料Manual substitution
S10	C5	淳安千岛湖桑都食用菌合作社Chun’an Qiandao Lake Sangdu Edible Fungus Cooperative	人工代料Manual substitution

编号No.	相似度Similarity
S1	0.966
S2	0.985
S3	0.989
S4	0.975
S5	0.942
S6	0.984
S7	0.924
S8	0.955
S9	0.968
S10	0.973

编号No.	相似度Similarity
S1	0.966
S2	0.985
S3	0.989
S4	0.975
S5	0.942
S6	0.984
S7	0.924
S8	0.955
S9	0.968
S10	0.973

成分 Component	特征值 Eigenvalue	方差的贡献率 Contribution rate of variance/%	累计的方差贡献率 Cumulative variance contribution rate/%
1	5.070	42.248	42.248
2	2.584	21.532	63.780
3	1.727	14.392	78.172
4	1.221	10.172	88.343

HPLC fingerprint of cultivated Sanghuangporus and content determination of main component

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Figures/Tables 12

References 18

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编号 No.	批号 Batch No.	峰面积 Peak area	含量 Content/%
S1	A1	2 082.7	0.21
S2	A2	4 129.5	0.45
S3	B1	5 992.5	0.66
S4	B2	8 471.5	0.95
S5	B3	1 564.4	0.15
S6	C1	2 138.3	0.21
S7	C2	3 107.8	0.33
S8	C3	2 169.5	0.22
S9	C4	3 696.9	0.39
S10	Y1	5 086.7	0.56

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