Identification and validation of differential proteins in liver of New Zealand rabbits at different months of age

doi:10.3969/j.issn.1004-1524.20221467

Acta Agriculturae Zhejiangensis ›› 2023, Vol. 35 ›› Issue (10): 2299-2310.DOI: 10.3969/j.issn.1004-1524.20221467

• Animal Science • Previous Articles Next Articles

Identification and validation of differential proteins in liver of New Zealand rabbits at different months of age

LIU Fangcheng^a^,^b(), WANG Feng^a^,^b, BI Donglin^a^,^b, YANG Dongliang^a^,^b, YANG Xiaoli^a^,^b, BAI Jialin^a^,^b, LI Qiongyi^a^,^b^,^*()

a. Biomedical Research Center, Key Laboratory of Biotechnology and Bioengineering of State Ethnic Affairs Commission, Northwest Minzu University, Lanzhou 730030, China
b. Biomedical Research Center, College of Life Science and Engineering, Northwest Minzu University, Lanzhou 730030, China

Received:2022-10-25 Online:2023-10-25 Published:2023-10-31

Abstract

Abstract:

Rabbit hemorrhagic disease is an acute and highly fatal infectious disease caused by Rabbit hemorrhagic disease virus (RHDV). The mortality rate of adult rabbits is more than 90%, but 2-week-old young rabbits are not affected. Based on the differences in the susceptibility of young rabbits and adult rabbits to RHDV, tandem mass tage (TMT) proteomics technology was used to detect and quantitatively analyze the proteins in liver of young rabbits (2 weeks old) and adult rabbits (6 months old). 4 353 proteins were quantitatively obtained from the liver of young rabbits and adult rabbits, and 821 differential proteins were screened. Among them, 294 proteins were significantly up-regulated and 527 proteins were significantly down-regulated in the liver of adult rabbits compared with young rabbits. The results of GO functional enrichment analysis showed that 145 small molecule metabolic proteins, 111 redox-related proteins and 478 proteins involved in metabolic processes were mainly enriched in biological processes; 528 extracellular component proteins, 139 mitochondrial proteins and 366 cytoplasmic proteins were mainly enriched in cellular components; and 342 catalytic active proteins, 93 redox active proteins and 50 coenzyme binding proteins were mainly enriched in molecular functions. A total of 47 KEGG (Kyoto Encyclopedia of Genes and Genomes) signaling pathways were annotated in 821 liver differential proteins in the KEGG pathway database, mainly involving metabolic pathways, carbon metabolism, oxidative phosphorylation and splicing bodies. The analysis of differential protein interaction network in liver of young and adult rabbits showed that MRPS15 had the highest correlation degree, and the differential protein cholesterol acyltransferase 1 (ACAT1) had more interaction in the interaction network. To explore the mechanism of infection difference between young and adult rabbits from the protein level, and to screen and analyze the differential proteins in liver of young and adult rabbits, it is expected to become a breakthrough point in the construction of RHDV in vitro amplification cell lines, and provide new research ideas for the construction of cell lines suitable for RHDV in vitro amplification.

Key words: New Zealand rabbit, tandem mass tage (TMT) proteomics, rabbit hemorrhagic disease virus (RHDV), liver, differential proteins

CLC Number:

S855.3

LIU Fangcheng, WANG Feng, BI Donglin, YANG Dongliang, YANG Xiaoli, BAI Jialin, LI Qiongyi. Identification and validation of differential proteins in liver of New Zealand rabbits at different months of age[J]. Acta Agriculturae Zhejiangensis, 2023, 35(10): 2299-2310.

Figures/Tables 6

References 35

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蛋白质ID Protein ID	蛋白质名称 Protein name	表达情况 Expression
Q8MI17	醇脱氢酶Aldehyde dehydrogenase 1 family member A1	上调Up-regulation
G1SS18	角蛋白8Keratin 8	上调Up-regulation
G1SIJ2	胆固醇酰基转移酶1 Acetyl-CoA acetyltransferase 1	下调Down-regulation
G1U758	角蛋白18 Keratin 18	上调Up-regulation
G1TT06	组蛋白结合蛋白1 Selenium binding protein 1	上调Up-regulation
G1SRF7	热休克蛋白9 Heat shock protein family A member 9	下调Down-regulation
G1SKT4	ATP合成酶、H+转运、线粒体F1复合体、α亚单位1	下调Down-regulation
	ATP synthase, H+transporting, mitochondrial F1 complex, alpha subunit 1
A0A5F9CMG2	3-羟基-3-甲基戊二酰-CoA合成酶2 3-Hydroxy-3-methylglutaryl-CoA synthase 2	下调Down-regulation
G1SXX5	羟基酰基-CoA脱氢酶三功能多酶复合体亚基α	下调Down-regulation
	Hydroxyacyl-CoA dehydrogenase trifunctional multienzyme complex subunit alpha
G1SJS1	组蛋白2簇H2be Histone 2 cluster H2be	下调Down-regulation
A0A5F9C4D8	UDP葡萄糖焦磷酸化酶2 UDP-glucose pyrophosphorylase 2	上调Up-regulation
G1T1U7	亚甲基四氢叶酸脱氢酶	上调Up-regulation
	Methylenetetrahydrofolate dehydrogenase, cyclohydrolase and formyltetrahydrofolate synthetase 1
U3KMH9	苹果酸脱氢酶2 Malate dehydrogenase 2	下调Down-regulation
P21195	脯氨酰4-羟化酶亚基beta Prolyl 4-hydroxylase subunit beta	下调Down-regulation
O18750	热休克蛋白90 beta家族成员1 Heat shock protein 90 beta family member 1	下调Down-regulation
G1T2A7	谷胱甘肽S转移酶α1 Glutathione S-transferase alpha I	上调Up-regulation
G1T295	环氧化物水解酶1 Epoxide hydrolase 1	上调Up-regulation
G1U723	前列腺素-E(2) 9还原酶样Prostaglandin-E(2) 9-reductase-like	上调Up-regulation
A0A5F9C5G3	丝氨酸羟甲基转移酶1 Serine hydroxymethyltransferase 1	上调Up-regulation
G1SNJ7	丙氨酸-乙醛酸氨基转移酶2 Alanine-glyoxylate aminotransferase 2	下调Down-regulation

蛋白质ID Protein ID	蛋白质名称 Protein name	表达情况 Expression
Q8MI17	醇脱氢酶Aldehyde dehydrogenase 1 family member A1	上调Up-regulation
G1SS18	角蛋白8Keratin 8	上调Up-regulation
G1SIJ2	胆固醇酰基转移酶1 Acetyl-CoA acetyltransferase 1	下调Down-regulation
G1U758	角蛋白18 Keratin 18	上调Up-regulation
G1TT06	组蛋白结合蛋白1 Selenium binding protein 1	上调Up-regulation
G1SRF7	热休克蛋白9 Heat shock protein family A member 9	下调Down-regulation
G1SKT4	ATP合成酶、H+转运、线粒体F1复合体、α亚单位1	下调Down-regulation
	ATP synthase, H+transporting, mitochondrial F1 complex, alpha subunit 1
A0A5F9CMG2	3-羟基-3-甲基戊二酰-CoA合成酶2 3-Hydroxy-3-methylglutaryl-CoA synthase 2	下调Down-regulation
G1SXX5	羟基酰基-CoA脱氢酶三功能多酶复合体亚基α	下调Down-regulation
	Hydroxyacyl-CoA dehydrogenase trifunctional multienzyme complex subunit alpha
G1SJS1	组蛋白2簇H2be Histone 2 cluster H2be	下调Down-regulation
A0A5F9C4D8	UDP葡萄糖焦磷酸化酶2 UDP-glucose pyrophosphorylase 2	上调Up-regulation
G1T1U7	亚甲基四氢叶酸脱氢酶	上调Up-regulation
	Methylenetetrahydrofolate dehydrogenase, cyclohydrolase and formyltetrahydrofolate synthetase 1
U3KMH9	苹果酸脱氢酶2 Malate dehydrogenase 2	下调Down-regulation
P21195	脯氨酰4-羟化酶亚基beta Prolyl 4-hydroxylase subunit beta	下调Down-regulation
O18750	热休克蛋白90 beta家族成员1 Heat shock protein 90 beta family member 1	下调Down-regulation
G1T2A7	谷胱甘肽S转移酶α1 Glutathione S-transferase alpha I	上调Up-regulation
G1T295	环氧化物水解酶1 Epoxide hydrolase 1	上调Up-regulation
G1U723	前列腺素-E(2) 9还原酶样Prostaglandin-E(2) 9-reductase-like	上调Up-regulation
A0A5F9C5G3	丝氨酸羟甲基转移酶1 Serine hydroxymethyltransferase 1	上调Up-regulation
G1SNJ7	丙氨酸-乙醛酸氨基转移酶2 Alanine-glyoxylate aminotransferase 2	下调Down-regulation

通路名称 Pathway name	ID	差异蛋白数量 Number of differential proteins
代谢途径Metabolic pathways	ocu01100	179
糖代谢Carbon metabolism	ocu01200	30
氧化磷酸化Oxidative phosphorylation	ocu00190	29
帕金森氏症Parkinson disease	ocu05012	29
剪切小体Spliceosome	ocu03040	26
阿兹海默病Alzheimer disease	ocu05010	30
丙酸代谢Propanoate metabolism	ocu00640	12
乙氧基化物和二甲酸酯代谢通路Glyoxylate and dicarboxylate metabolism	ocu00630	11
类固醇生物合成Steroid biosynthesis	ocu00100	8
丁酸盐代谢通路Butanoate metabolism	ocu00650	9

通路名称 Pathway name	ID	差异蛋白数量 Number of differential proteins
代谢途径Metabolic pathways	ocu01100	179
糖代谢Carbon metabolism	ocu01200	30
氧化磷酸化Oxidative phosphorylation	ocu00190	29
帕金森氏症Parkinson disease	ocu05012	29
剪切小体Spliceosome	ocu03040	26
阿兹海默病Alzheimer disease	ocu05010	30
丙酸代谢Propanoate metabolism	ocu00640	12
乙氧基化物和二甲酸酯代谢通路Glyoxylate and dicarboxylate metabolism	ocu00630	11
类固醇生物合成Steroid biosynthesis	ocu00100	8
丁酸盐代谢通路Butanoate metabolism	ocu00650	9

Identification and validation of differential proteins in liver of New Zealand rabbits at different months of age

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