New dwarf mutants of oilseed rape (Brassica napus L.) induced by chemical mutagenesis

doi:10.3969/j.issn.1004-1524.20221564

Acta Agriculturae Zhejiangensis ›› 2023, Vol. 35 ›› Issue (11): 2516-2524.DOI: 10.3969/j.issn.1004-1524.20221564

• Crop Science • Previous Articles Next Articles

New dwarf mutants of oilseed rape (Brassica napus L.) induced by chemical mutagenesis

LANG Chunxiu^a(), LIU Renhu^a, ZHENG Tao^a, WANG Fulin^a, SHI Jianghua^b, HU Zhanghua^a, WU Guanting^a^,^*()

a. Institute of Virology and Biotechnology; b. Institute of Crops and Nuclear Technology Utilization, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China

Received:2022-11-03 Online:2023-11-25 Published:2023-12-04

Abstract

Abstract:

The discovery and creation of dwarf germplasm resources of oilseed rape (Brassica napus L.) are of great significance for developing new varieties with moderate plant dwarfing, strong lodging resistance, high and stable yield, and good suitability for mechanized harvesting. In this study, two dwarf mutants 3060 and 4036 were obtained by treating seeds of Brassica napus cultivar Zheyou 50 with the chemical mutagen ethyl methane sulphonate (EMS). The two mutants were 74.5 cm and 72.0 cm in plant height respectively, all with a compact and upright plant type. Many phenotypic characteristics at seedling and maturation stages as well as quality traits including seed fatty acid composition and oil content in the two mutants were significantly changed compared with the original parent. The results of genetic analysis preliminarily showed that the two dwarf mutations were controlled by a single recessive gene or a recessive major gene without cytoplasmic effects. Seedling morphogenesis of the two mutants was normal under light and dark conditions, suggesting that their dwarfing mechanism was unrelated to brassinosteroids pathway. Seedling hypocotyl elongation of the two mutants could be significantly promoted by spraying different concentrations of exogenous gibberellin, but hypocotyl length could not be restored to the level of the original parent, indicating that the occurrence of dwarf mutations might be related to variation(s) in the gibberellin signal transduction pathway. The two dwarf mutants were believed to have a certain utilization value because of their some important characteristics.

Key words: Brassica napus L., ethyl methane sulphonate (EMS), dwarf mutant, genetic analysis, gibberellin

CLC Number:

S634.3

LANG Chunxiu, LIU Renhu, ZHENG Tao, WANG Fulin, SHI Jianghua, HU Zhanghua, WU Guanting. New dwarf mutants of oilseed rape (Brassica napus L.) induced by chemical mutagenesis[J]. Acta Agriculturae Zhejiangensis, 2023, 35(11): 2516-2524.

Figures/Tables 6

References 36

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材料 Material	株高 Plant height/ cm	一次分枝位 Position of primary branches/cm	一次分枝数 Number of primary branches	二次分枝数 Number of secondary branches	每株角数 Number of pods per plant	角果长度 Pod length/ cm	每角粒数 Number of grains per pod	千粒重 1 000-grain weight/g	单株产量 Yield per plant/g	开花期(月-日) Flowering date (month-day)
3060	74.5^**	27.7^*	6.9	4.1	327.9^*	3.7^**	17.0^*	4.62^**	24.8^*	03-28
4036	72.0^**	17.9^**	6.3^*	6.1	359.0	3.4^**	16.1^*	3.27^**	17.7^**	03-31
ZY50	154.7	38.5	9.2	7.4	411.6	6.9	20.1	4.03	30.3	03-20

材料 Material	株高 Plant height/ cm	一次分枝位 Position of primary branches/cm	一次分枝数 Number of primary branches	二次分枝数 Number of secondary branches	每株角数 Number of pods per plant	角果长度 Pod length/ cm	每角粒数 Number of grains per pod	千粒重 1 000-grain weight/g	单株产量 Yield per plant/g	开花期(月-日) Flowering date (month-day)
3060	74.5^**	27.7^*	6.9	4.1	327.9^*	3.7^**	17.0^*	4.62^**	24.8^*	03-28
4036	72.0^**	17.9^**	6.3^*	6.1	359.0	3.4^**	16.1^*	3.27^**	17.7^**	03-31
ZY50	154.7	38.5	9.2	7.4	411.6	6.9	20.1	4.03	30.3	03-20

材料 Material	脂肪酸组成Fatty acid composition							含油量 Oil content
材料 Material	C16:0	C18:0	C18:1	C18:2	C18:3	C20:1	C22:1	含油量 Oil content
3060	3.83^**	1.22^*	41.15^**	14.28^**	5.35^**	13.74^**	19.44^**	42.3^**
4036	4.82	2.08^**	68.09	18.26^*	5.33^**	0.75	0.02	41.8^**
ZY50	4.61	1.45	67.81	17.61	7.28	0.72	0.01	49.2

材料 Material	脂肪酸组成Fatty acid composition							含油量 Oil content
材料 Material	C16:0	C18:0	C18:1	C18:2	C18:3	C20:1	C22:1	含油量 Oil content
3060	3.83^**	1.22^*	41.15^**	14.28^**	5.35^**	13.74^**	19.44^**	42.3^**
4036	4.82	2.08^**	68.09	18.26^*	5.33^**	0.75	0.02	41.8^**
ZY50	4.61	1.45	67.81	17.61	7.28	0.72	0.01	49.2

组合 Combination	调查苗数 Number of seedlings investigated	正常苗数 Number of normal seedlings	矮生苗数 Number of dwarf seedlings	分离比 Segregation ratio	期望比率 Expected ratio	卡平方值 Chi-square value	概率值 Probability value
3060/ZY50	1 252	956	296	3.23:1	3:1	1.231	0.267
ZY50/3060	1 114	809	305	2.65:1	3:1	3.362	0.067
3060/ZS72	1 158	880	278	3.17:1	3:1	0.609	0.435
ZS72/3060	1 087	839	248	3.38:1	3:1	2.768	0.096
4036/ZY50	1 056	783	273	2.87:1	3:1	0.409	0.522
ZY50/4036	1 209	930	279	3.33:1	3:1	2.385	0.123
4036/ZS72	1 021	794	227	3.50:1	3:1	4.169	0.041
ZS72/4036	943	690	253	2.73:1	3:1	1.683	0.195

New dwarf mutants of oilseed rape (Brassica napus L.) induced by chemical mutagenesis

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