Tissue culture and breeding of Pleione formosana Hayata and chemical composition analysis of its pseudobulbs

doi:10.3969/j.issn.1004-1524.20230370

Acta Agriculturae Zhejiangensis ›› 2024, Vol. 36 ›› Issue (2): 358-364.DOI: 10.3969/j.issn.1004-1524.20230370

• Horticultural Science • Previous Articles Next Articles

Tissue culture and breeding of Pleione formosana Hayata and chemical composition analysis of its pseudobulbs

SUN Jian(), REN Jiangjian^*(), WANG Haige, JIANG Jianming, WANG Zhi’an, YU Xuping

Zhejiang Research Institute of Traditional Chinese Medicine Co., Ltd., Hangzhou 310023, China

Received:2023-03-20 Online:2024-02-25 Published:2024-03-05

Abstract

Abstract:

The aim of this study was to construct the tissue culture and breeding technology system of Pleione formosana Hayata, and analyze the chemical composition characteristics of pseudobulbs of P. formosana Hayata. After seed germination, protocorm growth, pseudobulb proliferation, rooting and seedling culture, the plants of P. formosana Hayata were obtained. The pseudobulbs of tissue-cultured P. formosana Hayata plants were used as materials. The chemical composition of pseudobulbs was analyzed by non-targeted metabolomics and HPLC fingerprint. After 2 years of breeding, more than 1 000 seedlings with about 0.5 g pseudobulbs were obtained from a single capsule. Through the analysis of non-targeted metabolomics data, 1 229 common compounds were identified in different materials, including 236 carboxylic acids and their derivatives, 110 benzene and substituted derivatives, 106 organic oxygen compounds, 94 fatty acyl compounds, 43 flavonoids, and 42 steroids and steroid derivatives. Among them, the benzyl esters represented by 1,4-bis [4-(glucoseoxy) benzyl]-2-isobutylmalate are orchid-enriched compounds. Meanwhile the content of 1,4-bis [4-(glucoxy ) benzyl]-2-isobutylmalate was 0.53% in pseudobulbs detected by HPLC. The plants of P. formosana Hayata can be bred by tissue culture technology. And the pseudobulbs of P. formosana Hayata are rich in benzyl ester compounds as well as P. bulbocodioides(Franch.) Rolfe.

Key words: Pleione formosana Hayata, tissue culture breeding, chemical composition

CLC Number:

S682.31

SUN Jian, REN Jiangjian, WANG Haige, JIANG Jianming, WANG Zhi’an, YU Xuping. Tissue culture and breeding of Pleione formosana Hayata and chemical composition analysis of its pseudobulbs[J]. Acta Agriculturae Zhejiangensis, 2024, 36(2): 358-364.

Figures/Tables 4

References 15

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激素处理 Hormone treatment/(mg·L^-1)	增殖倍数 Proliferation rate	球状茎形态 Spherical bulbous	球状茎质量 Bulbous weight/g
6-BA 1.0+NAA 0.2	1.3±0.2 e	细小Slender	0.06±0.01 d
6-BA 1.5+NAA 0.2	2.6±0.4 d	细小Slender	0.08±0.01 c
6-BA 2.0+NAA 0.2	3.4±0.3 c	中等Medium	0.10±0.01 b
6-BA 2.5+NAA 0.2	5.7±0.3 a	粗壮Thick	0.13±0.02 a
6-BA 3.0+NAA 0.2	4.3±0.5 bc	中等Medium	0.11±0.02 ab
6-BA 3.5+NAA 0.2	3.8±0.4 c	中等Medium	0.09±0.02 b

激素处理 Hormone treatment/(mg·L^-1)	增殖倍数 Proliferation rate	球状茎形态 Spherical bulbous	球状茎质量 Bulbous weight/g
6-BA 1.0+NAA 0.2	1.3±0.2 e	细小Slender	0.06±0.01 d
6-BA 1.5+NAA 0.2	2.6±0.4 d	细小Slender	0.08±0.01 c
6-BA 2.0+NAA 0.2	3.4±0.3 c	中等Medium	0.10±0.01 b
6-BA 2.5+NAA 0.2	5.7±0.3 a	粗壮Thick	0.13±0.02 a
6-BA 3.0+NAA 0.2	4.3±0.5 bc	中等Medium	0.11±0.02 ab
6-BA 3.5+NAA 0.2	3.8±0.4 c	中等Medium	0.09±0.02 b

激素处理 Hormone treatment/(mg·L^-1)	生根率 Rooting rate/%	幼苗形态 Seedling morphology	假鳞茎质量 Pseudobulb weight/g
NAA 0.2+6-BA 0.2+IBA 1.0+2.4-D 0.01	83.0±6.0 b	中等Medium	0.41±0.06 b
NAA 0.3+6-BA 0.3+IBA 1.0+2.4-D 0.01	99.0±1.0 a	纤细Slender	0.31±0.02 c
NAA 0.5+6-BA 0.5+IBA 1.0+2.4-D 0.01	100.0±0.0a	粗壮Thick	0.52±0.05 a
NAA 0.8+6-BA 0.8+IBA 1.0+2.4-D 0.01	95.0±4.0 ab	中等Medium	0.37±0.04 b
NAA 1.0+6-BA 1.0+IBA 1.0+2.4-D 0.01	87.0±5.0 b	粗壮Thick	0.48±0.05 a

激素处理 Hormone treatment/(mg·L^-1)	生根率 Rooting rate/%	幼苗形态 Seedling morphology	假鳞茎质量 Pseudobulb weight/g
NAA 0.2+6-BA 0.2+IBA 1.0+2.4-D 0.01	83.0±6.0 b	中等Medium	0.41±0.06 b
NAA 0.3+6-BA 0.3+IBA 1.0+2.4-D 0.01	99.0±1.0 a	纤细Slender	0.31±0.02 c
NAA 0.5+6-BA 0.5+IBA 1.0+2.4-D 0.01	100.0±0.0a	粗壮Thick	0.52±0.05 a
NAA 0.8+6-BA 0.8+IBA 1.0+2.4-D 0.01	95.0±4.0 ab	中等Medium	0.37±0.04 b
NAA 1.0+6-BA 1.0+IBA 1.0+2.4-D 0.01	87.0±5.0 b	粗壮Thick	0.48±0.05 a

Tissue culture and breeding of Pleione formosana Hayata and chemical composition analysis of its pseudobulbs

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