Interference of LED green light on the feeding behavior of Empoasca onukii Matsuda

doi:10.3969/j.issn.1004-1524.20240268

Acta Agriculturae Zhejiangensis ›› 2024, Vol. 36 ›› Issue (9): 2051-2059.DOI: 10.3969/j.issn.1004-1524.20240268

• Plant Protection • Previous Articles Next Articles

Interference of LED green light on the feeding behavior of Empoasca onukii Matsuda

QIAO Li¹(), CHEN Lei¹, JIANG Yueli², ZHOU Zhou¹, GENG Shubao¹, WANG Lijuan³^,^*()

1. School of Agronomy, Xinyang Agriculture and Forestry University, Xinyang 464000, Henan, China
2. Institute of Plant Protection, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China
3. Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, China

Received:2024-03-27 Online:2024-09-25 Published:2024-09-30

Abstract

Abstract:

Empoasca onukii Matsuda is one of the common pests in tea areas in China, and its harm seriously affects the yield and quality of tea. In order to reduce the residues of chemical pesticides and explore new control methods of leafhoppers in tea garden, LED green light (wavelength 520-525 nm) was used to interfere the feeding process of E. onukii, which was sensitive to light, the effects of light intensity and irradiation time on the feeding behavior of E. onukii were analyzed by means of electrical penetration graph (EPG) recording. The results showed that, the waveform produced by the feeding process of E. onukii were NP wave, A wave, C wave, E wave, S wave, F wave and R wave, among which NP wave accounted for the largest proportion. Compared with natural light (control), LED green light significantly inhibited the feeding behavior of E. onukii. At the same time, the higher the light intensity, the lower the percentage of spiking, and the more stinging and sucking times, the more obvious interference to the feeding behavior of E. onukii. The results of this study could provide technical support for the application of light prevention and control of leafhopper in tea garden.

Key words: LED green light, feeding behavior, Empoasca onukii Matsuda, electrical penetration graph (EPG)

CLC Number:

S433.3

QIAO Li, CHEN Lei, JIANG Yueli, ZHOU Zhou, GENG Shubao, WANG Lijuan. Interference of LED green light on the feeding behavior of Empoasca onukii Matsuda[J]. Acta Agriculturae Zhejiangensis, 2024, 36(9): 2051-2059.

Figures/Tables 6

References 30

[1]	孟召娜, 边磊, 罗宗秀, 等. 全国主产茶区茶树小绿叶蝉种类鉴定及分析[J]. 应用昆虫学报, 2018, 55(3): 514-526.
	MENG Z N, BIAN L, LUO Z X, et al. Taxonomic revision and analysis of the green tea leafhopper species in China’s main tea production area[J]. Chinese Journal of Applied Entomology, 2018, 55(3): 514-526. (in Chinese with English abstract)
[2]	秦道正, 肖强, 王玉春, 等. 危害陕西茶区茶树的小绿叶蝉种类订正及对我国茶树小绿叶蝉的再认识[J]. 西北农林科技大学学报(自然科学版), 2014, 42(5): 124-134.
	QIN D Z, XIAO Q, WANG Y C, et al. Revision of green leafhopper species damaging tea shrub in Shaanxi and reconsideration of the species in China[J]. Journal of Northwest A & F University(Natural Science Edition), 2014, 42(5): 124-134. (in Chinese with English abstract)
[3]	乔利, 张方梅, 张权, 等. 小贯小绿叶蝉试验种群生命表研究[J]. 江苏农业科学, 2019, 47(3): 100-103.
	QIAO L, ZHANG F M, ZHANG Q, et al. Study on life table of laboratory population of Empoasca onukii Matsuda[J]. Jiangsu Agricultural Sciences, 2019, 47(3): 100-103. (in Chinese)
[4]	乔利, 秦道正, 卢兆成, 等. 温度对茶小绿叶蝉成虫存活率及保护酶系的影响[J]. 植物保护学报, 2015, 42(2): 223-228.
	QIAO L, QIN D Z, LU Z C, et al. Effects of temperature on survival rate and protection enzymes of Empoasca onukii Matsuda[J]. Journal of Plant Protection, 2015, 42(2): 223-228. (in Chinese with English abstract)
[5]	乔利, 金银利, 蒋月丽, 等. 光谱对小贯小绿叶蝉趋光行为的影响[J]. 环境昆虫学报, 2022, 44(5): 1197-1204.
	QIAO L, JIN Y L, JIANG Y L, et al. Effects of spectra on phototactic behavior of the Empoasca onukii Matsuda[J]. Journal of Environmental Entomology, 2022, 44(5): 1197-1204. (in Chinese with English abstract)
[6]	邬子惠, 王梦馨, 潘铖, 等. 茶小绿叶蝉发生规律与绿色防控研究进展[J]. 茶叶通讯, 2021, 48(2): 200-206.
	WU Z H, WANG M X, PAN C, et al. Research progress and prospect of occurrence regularity and green control of tea green leafhopper[J]. Journal of Tea Communication, 2021, 48(2): 200-206. (in Chinese with English abstract)
[7]	孟召娜, 边磊, 罗宗秀, 等. 茶园小贯小绿叶蝉各虫龄形态特征[J]. 中国茶叶, 2018, 40(12): 29-31.
	MENG Z N, BIAN L, LUO Z X, et al. Morphological characteristics of different instars of Empoasca onnkii Matsuda in tea garden[J]. China Tea, 2018, 40(12): 29-31. (in Chinese)
[8]	陈晶, 朱启兵, 黄敏, 等. 基于机器视觉的茶小绿叶蝉识别方法研究[J]. 激光与光电子学进展, 2018, 55(1): 011502.
	CHEN J, ZHU Q B, HUANG M, et al. Recognition of Empoasca flavescens based on machine vision[J]. Laser & Optoelectronics Progress, 2018, 55(1): 011502. (in Chinese with English abstract)
[9]	刘燕. 灯光防治害虫技术的应用现状和发展趋势[J]. 中国农机化, 2012(4): 34-36.
	LIU Y. Application status and development trends of the lighting pests control technology[J]. Chinese Agricul Tural Mechanization, 2012(4): 34-36. (in Chinese with English abstract)
[10]	杨现明, 陆宴辉, 梁革梅. 昆虫趋光行为及灯光诱杀技术[J]. 照明工程学报, 2020, 31(5): 22-31.
	YANG X M, LU Y H, LIANG G M. Insect phototaxis behavior and light trapping technology[J]. China Illuminating Engineering Journal, 2020, 31(5): 22-31. (in Chinese with English abstract)
[11]	SHI L Q, HE H F, YANG G, et al. Are yellow sticky cards and light traps effective on tea green leafhoppers and their predators in Chinese tea plantations?[J]. Insects, 2020, 12(1): 14.
[12]	边磊, 陈宗懋, 陈华才, 等. 新型LED杀虫灯对茶园昆虫的诱杀效果评价[J]. 中国茶叶, 2016, 38(6): 22-23.
	BIAN L, CHEN Z M, CHEN H C, et al. Evaluation of trapping and killing effect of new LED insecticidal lamp on tea garden insects[J]. China Tea, 2016, 38(6): 22-23. (in Chinese)
[13]	施龙清. 福建茶小绿叶蝉种类鉴定及其对光的行为响应[D]. 福州: 福建农林大学, 2016.
	SHI L Q. Species identification of tea leafhopper in Fujian and its behavioral response to light[D]. Fuzhou: Fujian Agriculture and Forestry University, 2016. (in Chinese with English abstract)
[14]	陈伟忠, 黄伙水, 陈秀辉, 等. 光对茶园小贯小绿叶蝉卵孵化的影响[J]. 蚕桑茶叶通讯, 2016(4): 22-24.
	CHEN W Z, HUANG H S, CHEN X H, et al. Effect of light on the hatching of the Empoasca onukii Matsuda eggs in tea garden[J]. Newsletter of Sericulture and Tea, 2016(4): 22-24. (in Chinese)
[15]	金珊, 孙晓玲, 陈宗懋, 等. 昆虫刺探电位图谱(EPG)技术在茶树抗刺吸式口器害虫研究中的应用[J]. 茶叶科学, 2012, 32(5): 393-401.
	JIN S, SUN X L, CHEN Z M, et al. Applications of electric penetration graph (EPG) technique in the research of resistance of tea plant to piercing-sucking insects[J]. Journal of Tea Science, 2012, 32(5): 393-401. (in Chinese with English abstract)
[16]	BACKUS E A, RANGASAMY M, STAMM M, et al. Waveform library for chinch bugs (Hemiptera: Heteroptera: Blissidae): characterization of electrical penetration graph waveforms at multiple input impedances[J]. Annals of the Entomological Society of America, 2013, 106(4): 524-539.
[17]	CERVANTES F A, BACKUS E A. EPG waveform library for Graphocephala atropunctata(Hemiptera: Cicadellidae): effect of adhesive, input resistor, and voltage levels on waveform appearance and stylet probing behaviors[J]. Journal of Insect Physiology, 2018, 109: 21-40.
[18]	于玮台, 陈文龙. 刺探电位图谱(EPG)技术在茶树半翅目害虫上的应用及展望[J]. 山地农业生物学报, 2012, 31(6): 552-556.
	YU W T, CHEN W L. Application and outlook of electrical penetration graph (EPG) to pests of Hemiptera in tea tree[J]. Journal of Mountain Agriculture and Biology, 2012, 31(6): 552-556. (in Chinese with English abstract)
[19]	李静静, 潘建斌, 吴莉莉, 等. 我国刺吸电位技术三十年应用及创新[J]. 应用昆虫学报, 2019, 56(6): 1224-1234.
	LI J J, PAN J B, WU L L, et al. Three-decades of electrical penetration graph technique innovation in China[J]. Chinese Journal of Applied Entomology, 2019, 56(6): 1224-1234. (in Chinese with English abstract)
[20]	胡想顺, 刘小凤, 赵惠燕. 刺探电位图谱(EPG)技术的原理与发展[J]. 植物保护, 2006, 32(3): 1-4.
	HU X S, LIU X F, ZHAO H Y. Development and application of electrical penetration graph (EPG) technique[J]. Plant Protection, 2006, 32(3): 1-4. (in Chinese with English abstract)
[21]	苗进, 韩宝瑜. 假眼小绿叶蝉(Empoasca vitis Göthe)在不同品种茶树上的取食行为[J]. 生态学报, 2007, 27(10): 3973-3982.
	MIAO J, HAN B Y. The probing behavior of the tea green leafhopper on different tea plant cultivars[J]. Acta Ecologica Sinica, 2007, 27(10): 3973-3982. (in Chinese with English abstract)
[22]	郑雨婷, 王梦馨, 崔林, 等. 基于EPG技术分析茶树品种对茶小绿叶蝉的抗性及其相关的抗性物质[J]. 生态学报, 2017, 37(23): 8015-8028.
	ZHENG Y T, WANG M X, CUI L, et al. Resistance of tea cultivars to the tea green leafhopper analyzed by EPG technique and their resistance-related substances[J]. Acta Ecologica Sinica, 2017, 37(23): 8015-8028. (in Chinese with English abstract)
[23]	YANG J, LUO F J, ZHOU L, et al. Residue reduction and risk evaluation of chlorfenapyr residue in tea planting, tea processing, and tea brewing[J]. Science of the Total Environment, 2020, 738: 139613.
[24]	WANG X R, ZHANG X Z, WANG Z H, et al. Dissipation behavior and risk assessment of tolfenpyrad from tea bushes to consuming[J]. Science of the Total Environment, 2022, 806: 150771.
[25]	龙家寰, 张维强, 张盈, 等. 丁醚脲及其代谢物和甲维盐在茶树上的残留及消解动态[J]. 农药, 2023, 62(1): 43-48.
	LONG J H, ZHANG W Q, ZHANG Y, et al. Residue and dissipation dynamics of diafenthiuron, its metabolites and emamectin benzoate in tea tree[J]. Agrochemicals, 2023, 62(1): 43-48. (in Chinese with English abstract)
[26]	邹佳婷, 郭宇航, 边磊, 等. 化学农药对茶小绿叶蝉成虫的防效及其原因探究[J]. 茶叶科学, 2023, 43(4): 544-552.
	ZOU J T, GUO Y H, BIAN L, et al. Study on the control effect of chemical pesticides on the Empoasca onukii adults[J]. Journal of Tea Science, 2023, 43(4): 544-552. (in Chinese with English abstract)
[27]	王朝伟, 杨云秋, 钱六九, 等. 不同频振光源对皖南茶园主要昆虫的诱集效果[J]. 安徽农业大学学报, 2018, 45(5): 790-795.
	WANG C W, YANG Y Q, QIAN L J, et al. Trapping effect of different light sources from frequency trembler grid lamp on major insects in tea fields of southern Anhui[J]. Journal of Anhui Agricultural University, 2018, 45(5): 790-795. (in Chinese with English abstract)
[28]	JIN S, CHEN Z M, BACKUS E A, et al. Characterization of EPG waveforms for the tea green leafhopper, Empoasca vitis Göthe (Hemiptera: Cicadellidae), on tea plants and their correlation with stylet activities[J]. Journal of Insect Physiology, 2012, 58(9): 1235-1244.
[29]	刘丽芳. 茶树不同品种和次生代谢物质对叶蝉取食行为影响的DC-EPG研究[D]. 北京: 中国农业科学院, 2011.
	LIU L F. DC-EPG study on the effects of different tea varieties and secondary metabolites on the feeding behavior of leafhoppers[D]. Beijing: Chinese Academy of Agricultural Sciences, 2011. (in Chinese with English abstract)
[30]	张丽, 周冬, 杨杰, 等. UV-B胁迫小麦上麦长管蚜的生命表参数和取食行为[J]. 昆虫学报, 2013, 56(6): 665-670.
	ZHANG L, ZHOU D, YANG J, et al. Life table parameters and feeding behavior of Sitobion avenae(Hemiptera: Aphididae) on wheat under UV-B stress[J]. Acta Entomologica Sinica, 2013, 56(6): 665-670. (in Chinese with English abstract)

光照强度 Light intensity/lx	A波 A wave	S波 S wave	C波 C wave	E波 E wave	F波 F wave	R波 R wave	NP波 NP wave
80	2.01±0.47 a	6.00±0.80 a	4.98±1.67 b	11.16±1.42 b	5.65±1.98 ab	4.57±1.47 ab	85.63±2.40 c
120	1.14±0.41 b	5.50±1.11 ab	6.44±1.49 b	10.52±0.90 b	6.19±2.94 a	1.52±1.12 c	88.70±2.56 c
160	1.12±0.66 b	5.05±0.94 ab	5.13±0.57 b	8.23±0.67 c	4.20±2.11 abc	1.35±0.22 c	94.92±3.55 b
200	1.20±0.34 b	3.90±0.83 bc	4.83±0.51 b	5.97±1.29 d	2.60±0.51 bc	1.79±1.21 c	99.71±2.53 b
240	0.97±0.36 b	2.86±0.83 c	1.78±0.31 c	3.90±0.42 e	2.18±0.73 c	2.94±1.38 bc	105.38±2.11 a
对照CK	1.61±0.69 ab	5.38±1.35 ab	8.54±1.72 a	15.86±0.62 a	5.05±2.81 abc	5.15±0.93 a	78.41±5.94 d

光照强度 Light intensity/lx	A波 A wave	S波 S wave	C波 C wave	E波 E wave	F波 F wave	R波 R wave	NP波 NP wave
80	2.01±0.47 a	6.00±0.80 a	4.98±1.67 b	11.16±1.42 b	5.65±1.98 ab	4.57±1.47 ab	85.63±2.40 c
120	1.14±0.41 b	5.50±1.11 ab	6.44±1.49 b	10.52±0.90 b	6.19±2.94 a	1.52±1.12 c	88.70±2.56 c
160	1.12±0.66 b	5.05±0.94 ab	5.13±0.57 b	8.23±0.67 c	4.20±2.11 abc	1.35±0.22 c	94.92±3.55 b
200	1.20±0.34 b	3.90±0.83 bc	4.83±0.51 b	5.97±1.29 d	2.60±0.51 bc	1.79±1.21 c	99.71±2.53 b
240	0.97±0.36 b	2.86±0.83 c	1.78±0.31 c	3.90±0.42 e	2.18±0.73 c	2.94±1.38 bc	105.38±2.11 a
对照CK	1.61±0.69 ab	5.38±1.35 ab	8.54±1.72 a	15.86±0.62 a	5.05±2.81 abc	5.15±0.93 a	78.41±5.94 d

光照强度 Light intensity/lx	A波 A wave	S波 S wave	C波 C wave	E波 E wave	F波 F wave	R波 R wave	NP波 NP wave
80	2.68±0.78 ab	8.62±1.11 a	8.46±2.23 b	26.06±2.34 ab	10.04±1.23 a	8.74±1.95 ab	175.41±5.21 d
120	1.94±0.38 b	7.48±0.86 ab	8.28±1.73 b	22.82±2.98 bc	8.67±1.84 ab	6.70±2.27 b	184.12±2.85 c
160	2.25±0.62 ab	6.84±1.07 ab	6.24±0.56 c	20.10±2.74 c	7.16±1.77 bc	6.57±1.45 b	190.85±3.70 bc
200	1.89±0.33 b	5.69±1.50 bc	6.93±0.91 bc	15.70±3.11 d	6.09±1.40 c	6.08±2.35 b	197.63±7.66 b
240	1.70±0.27 b	4.42±0.93 c	4.63±0.42 d	8.57±0.96 e	4.94±0.62 c	6.35±1.70 b	209.40±2.64 a
对照CK	3.02±1.07 a	8.09±1.57 a	11.13±0.66 a	27.28±2.92 a	6.03±1.03 c	11.39±2.34 a	173.07±3.53 d

光照强度 Light intensity/lx	A波 A wave	S波 S wave	C波 C wave	E波 E wave	F波 F wave	R波 R wave	NP波 NP wave
80	2.68±0.78 ab	8.62±1.11 a	8.46±2.23 b	26.06±2.34 ab	10.04±1.23 a	8.74±1.95 ab	175.41±5.21 d
120	1.94±0.38 b	7.48±0.86 ab	8.28±1.73 b	22.82±2.98 bc	8.67±1.84 ab	6.70±2.27 b	184.12±2.85 c
160	2.25±0.62 ab	6.84±1.07 ab	6.24±0.56 c	20.10±2.74 c	7.16±1.77 bc	6.57±1.45 b	190.85±3.70 bc
200	1.89±0.33 b	5.69±1.50 bc	6.93±0.91 bc	15.70±3.11 d	6.09±1.40 c	6.08±2.35 b	197.63±7.66 b
240	1.70±0.27 b	4.42±0.93 c	4.63±0.42 d	8.57±0.96 e	4.94±0.62 c	6.35±1.70 b	209.40±2.64 a
对照CK	3.02±1.07 a	8.09±1.57 a	11.13±0.66 a	27.28±2.92 a	6.03±1.03 c	11.39±2.34 a	173.07±3.53 d

光照强度 Light intensity/lx	A波 A wave	S波 S wave	C波 C wave	E波 E wave	F波 F wave	R波 R wave	NP波 NP wave
80	2.55±0.35 b	9.80±0.64 b	10.78±1.14 b	35.42±1.19 b	12.72±2.32 ab	10.96±1.10 bc	277.78±3.46 c
120	2.06±0.53 b	9.19±0.79 bc	11.31±0.42 b	31.02±5.93 b	11.95±4.48 ab	9.89±1.57 c	284.60±8.02 bc
160	2.16±0.34 b	8.48±0.35 c	9.19±0.33 c	23.06±1.63 c	13.29±3.29 ab	12.76±1.19 b	291.08±4.04 b
200	2.06±0.20 b	8.44±0.36 c	9.24±0.80 c	21.09±1.97 c	10.34±1.94 b	14.42±1.55 a	294.41±3.92 b
240	1.95±0.29 b	4.86±0.40 d	6.04±1.15 d	8.63±0.66 d	9.23±1.66 b	10.56±2.52 bc	318.75±2.20 a
对照CK	4.34±0.59 a	13.05±1.68 a	15.07±1.36 a	46.27±8.08 a	15.05±1.06 a	15.16±1.60 a	251.06±11.84 d

Interference of LED green light on the feeding behavior of Empoasca onukii Matsuda

RichHTML

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

Figures/Tables 6

References 30

Related Articles 3

Recommended Articles

Metrics

Comments

[1]	YANG Chun, MENG Zehong, LI Shuai, LIANG Sihui, QIAO Dahe, CHEN Zhengwu. Resistance of 12 tea cultivars to Dendrothrips minowai Priesner and Empoasca Onukii Matsuda and a preliminary identification of resistant components [J]. Acta Agriculturae Zhejiangensis, 2022, 34(8): 1713-1724.
[2]	ZHANG Yuxiu;ZHANG Zhijun;LYU Yaobin;*. Analysis of the feeding behavior of western flower thrips， Frankliniella occidentalis (Pergande) (Thysanoptera: Thripidae) on four host plants by electrical penetration graph [J]. , 2014, 26(3): 0-714721.
[3]	CHEN Li;CHEN Jian-ming;*;HE Yue-ping;ZHANG Jue-feng. Comparison of feeding behaviors of imidacloprid-resistant and -susceptible brown planthopper (BPH, Nilaparvata lugens Stl) on different rice varieties [J]. , 2011, 23(5): 0-931.