Abstract: To investigate the effects of single or combined sodium dodecyl sulfate (SDS) and Cu²⁺ pollution in Bufo gargarizans, the acute toxicity and joint toxicity tests of SDS, Cu²⁺ and Na⁺-K⁺-ATPase activity in liver under the subacute toxicities of single or combined SDS and Cu²⁺ were measured by using exposure experiment. The results showed that the LC₅₀ of SDS for 48, 72 and 96 h to Bufo gargarizans were 27.38, 26.45 and 25.57 mg·L^-1, respectively, and the LC₅₀ of Cu²⁺ for 48, 72 and 96 h were 0.475, 0.323 and 0.285 mg·L^-1, respectively. The toxicity of Cu²⁺ was higher than that of SDS. In addition, it also indicated that the joint toxicity of Cu²⁺ and SDS was synergistic at 48 h but antagonistic at 96 h. The results also indicated that single or combined treatments with Cu²⁺ and SDS could affect the Na⁺-K⁺-ATPase activity in liver of Bufo gargarizans, and the joint effect of the combined pollution on the Na⁺-K⁺-ATPase activity was synergistic．

Key words: copper, SDS, Bufo gargarizans, acute toxicity, joint toxicity, Na⁺-K⁺-ATPase