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25 January 2010, Volume 22 Issue 1 Previous Issue    Next Issue
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Genetic analysis and mapping of a mutant gene for high tillering and dwarf in rice (Oryza sativa L.) LI Ke-lei;WANG De-kai;*;TAO Yue-zhi 2010, 22(1):  0-5.  Abstract ( 1608 )   PDF (1176KB) ( 1758 )   A rice mutant, showing the phenotype of high-tillering and dwarfing, was discovered from a population derived from a transgenic japonica cultivar ‘Zhonghua11’. The genetic analysis indicated that the phenotype of the mutant was controlled by a single recessive gene, which was designated tentatively as htd2. The F2 population was developed from a cross between the mutant and an indica cultivar ‘Longtefu B’ and used to investigate the genetic model and map the gene. The htd2 gene was firstly mapped on the short arm of chromosome3 and further mapped on the genetic region between SSR marker RM6038 and RM5444, which was 400 kb in length． Related Articles | Metrics

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Preparation and regeneration of protoplasts from a potential barnyard grass biocontrol fungus Helminthosporium gramineum f. sp. echinochloae ZHANG Jian-ping;ZHU Kai;YANG Shuang;ZHOU Yong-jun;YU Liu-qing* 2010, 22(1):  0-19.  Abstract ( 2675 )   PDF (1058KB) ( 1475 )   Helminthosporium gramineum f. sp. echinochloae(HGE)is a promising biological control barnyard grass agent. To establish the genetic transformation system of HGE, the optimum conditions including mycelium incubation time, various compounding of enzymes and osmotic stabilizers for protoplast isolation and regeneration from HGE protoplasts were examined. The results indicated that protoplasts were optimally isolated when mycelia of HGE had been cultured for 6 h, and digested with 1% Drislase at 26℃ for 2 h in osmotic stabilizer of 0.7mol·L-1 NaCl solution. The maximum regeneration ratio was obtained on the R1 regeneration medium when the mycelia of HGE were inoculated after 16 h, and digested with 0.5% Drislase at 32℃ for 4 h in 0.7mol·L-1 NaCl solution． Related Articles | Metrics

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Establishment of RT-PCR method for detecting Duck Hepatitis Virus I WEI Xue-tao;ZHANG Xiao-yong;LI Yin;LIU Yu-zhuo;ZHANG Jing-feng 2010, 22(1):  0-13.  Abstract ( 1935 )   PDF (950KB) ( 1438 )   According to the conserved gene sequence of Duck Hepatitis Virus I(DHV I) in GenBank, one pair of the specific primers were designed. A rapid diagnostic technique of RT-PCR was established by optimizing the reaction condition. The DNA segments amplified were 632 bp. The experiments had proved that RT-PCR possessed a high specificity. And the sensitivity test results indicated that the RT-PCR was more sensitive than the conventional method. Detection of field samples from viral diseased ducks also confirmed that the method of RT-PCR could effectively identify the infection of DHV． Related Articles | Metrics

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Compatibility of interspecific hybridization between Cucurbita pepo L. and Cucurbita maxima D． ZHANG Fu-xian;ZHANG Xin-hui;CHEN Run-xing;XU Jian-xiang;XIANG Xiao-min 2010, 22(1):  0-29.  Abstract ( 967 )   PDF (823KB) ( 1405 )   In this experiment, 12 self-line materials of two main cultivars in Cucurbita were crossed. There were 72 reciprocal cross combinations. The compatibility difference between reciprocal crosses and different materials, and the features of hybrid progenies were studied. The results showed that C. pepo L. was only suitable to be female parent and C. maxima D. was only suitable to be male parent in carrying out interspecific hybridization between C.pepo L. and C. maxima D. The variability of crossing compatibility was indicated by differences in percentages of setting, embryo fruit rate and embryo rate. The hybrid infertility were related to their cross compatibility. The possibility of improving C. pepo L. by crossing with C. maxima D. was also discussed in the paper． Related Articles | Metrics

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Chemical quality characteristics of Hongda flue-cured tobacco PANG Tao;SONG Chun-man;FANG Dun-huang;DENG Jian-hua;DENG Yun-long 2010, 22(1):  0-35.  Abstract ( 1123 )   PDF (1983KB) ( 1481 )   Partial least-squares method was employed in the analysis on 102 common and main flavor related compounds of flue-cured tobacco between Hongda and other varieties of flue-cured tobacco such as K326, Yunyan85 and Yunyan87. The result of cluster analysis showed that there was significant difference in certain chemical components between Hongda and other varieties of flue-cured tobacco. Further data analysis indicated that polyphenol, malic acid, octadecanoic acid, linoleic acid, linolenic acid, neochlorogenic acid, chlorogenic acid, megastigmatrienone, 4-O-caffeoylquinic acid, rutin, kaempferol-3-O-rutinoside, furan-2(5H)-one,4-methyl, tetradecyl aldehyde, tetradecanoic acid, solavetivone and thunbergol were the main compounds that were closely related to the chemical quality characteristics of Hongda flue-cured tobacco． Related Articles | Metrics

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Effects of chloride dioxide on biofilm formation by Salmonella MAO Jin-lin;GAO Hai-yan*;CHEN Hang-jun;GE Lin-mei;CHEN Qiu-yun 2010, 22(1):  0-113.  Abstract ( 897 )   PDF (1184KB) ( 1303 )   Biofilm formation of Salmonella sp. (AS 1.1552) on stainless steel surface were observed with scanning electronic microscope (SEM) and measured through its growth curve. The results showed that Salmonella sp. biofilm on stainless steel surface entered the stationary phase after 8 h incubation and the cell density was 3×106 cfu·cm-2. The bacterial cells on the biofilm had higher resistance to chlorine dioxide than those in suspension. Tryptone soy broth (TSB) obviously reduced the sterilization effect of chloride dioxide． Related Articles | Metrics

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Effect of shading on growth and diurnal photosynthetic changes of four vegetables in glasshouse DING Xiao-tao;JIN Hai-jun;ZHANG Hong-mei;YU Ji-zhu* 2010, 22(1):  0-56.  Abstract ( 2052 )   PDF (1178KB) ( 1988 )   Effect of different treatments of shading (glasshouse natural light, shading with one-layer shade net and two-layer shade net) on growth and the diurnal changes of photosynthesis in cucumber, pepper, tomato and eggplant was studied in glasshouse. The results indicated that dry matter accumulations of the four vegetables were greatly decreased by shading, and reduced most when shaded with two-layer shade net. Photosynthesis of cucumber, pepper and tomato was depressed at 9:00 under glasshouse natural light treatment. Moreover, the average whole-day photosynthetic rate was highest under one-layer shading treatment, the treatment with glasshouse natural light took the second place, and the photosynthetic rate was least when treated with two-layer shading. However, the photosynthetic rate of eggplant was glasshouse light treatment>one-layer shading treatment>two-layer shading treatment. The intercellular CO2 concentration of four vegetables with different treatments was two-layer shading treatment>one-layer shading treatment>glasshouse natural light treatment. Thus, stomatal limitation might be one of the factors leading to the photosynthesis depression in glasshouse natural light, meanwhile, non stomatal factor causing the lower ability of the whole plant leaf cell in utilizing CO2 was the main cause of the lower photosynthesis of two-layer shading treatment. Therefore, eggplant showed higher resistance to high light and high temperature stress. Related Articles | Metrics

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Effects of different nitrogen levels and forms on the appearance quality and photosynthesis characters of potted chrysanthemum with small inflorescences HUANG Chang-bing;FANG Wei-min;*;YANG Yong;CHEN Feng;XU Yao 2010, 22(1):  0-50.  Abstract ( 1885 )   PDF (1050KB) ( 1463 )   Substrate culture was employed to study the effects of different nitrogen levels (i.e., 1, 3, 8, 16, 26mmol·L-1) and different ratios of nitrogen forms (i.e., NH+4-N to NO-3-N, 4∶0, 1∶3, 2∶2, 3∶1, 0∶4) on appearance quality, chlorophyll content and photosynthesis characters of chrysanthemum with small inflorescences. The results showed that 16 mmol·L-1 nitrogen was optimal for vegetative growth, and it increased plant height, canopy, fresh weight and leaf numbers of plant. On the other hand, 8 mmol·L-1 nitrogen was optimal for reproductive growth, and it increased flower numbers, flower dry weight and prolonged the period of blooming. The flower development and its blooming were inhibited at higher level of nitrogen, but the chlorophyll content, net photosynthetic rate (Pn), stomatal conductance(Gs) and transpiration rate (Tr) increased and the intercellular CO2 concentration(Ci) decreased. Plant height, canopy, leaf numbers, flowers numbers, flower dry weight were enhanced under NH4+-N condition as compared to NO3--N. Furthermore, the chlorophyll content, net photosynthetic rate (Pn), transpiration rate (Tr) and stomatal conductance(Gs) increased, but the intercellular CO2 concentration (Ci) obviously decreased during the late period of vegetative growth under NH4+-N condition. However, nitrogen forms didn't affect the progress of flower development or the blooming period. It was found that a ratio of 3∶1 of NH4+-N∶NO3--N was optimal for potted chrysanthemum with small inflorescences． Related Articles | Metrics

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Effects of long-term located fertilization on yield and quality of sweetpotato TANG Zhong-hou;LI Hong-min;ZHANG Ai-jun;SHI Xin-min;ZHU Hong;SUN Jian 2010, 22(1):  0-61.  Abstract ( 948 )   PDF (829KB) ( 1683 )   Effects of long-term located fertilization on the yield and quality of sweetpotato in the monitoring base established in 1980 in Xuzhou were studied. The results showed that the application of organic fertilizer (M) could improve the yield more than other fertilizers, with the significant difference at P<0.01. The accumulation rates of starch and sugar under the condition of no fertilizer (CK) were faster than any other treatment with different fertilizations. The long-term fertilization was beneficial to the accumulation of starch, especially potassium(K), which was favorable for sweet potato variety with high starch. Nitrogen fertilizer(N)was useful for the C/N metabolism, but the ratio of dry matter assigned to the root was low under potassium deficiency. N could significantly increase the content of protein, which was useful for improving the quality of flesh-eating sweetpotato. There was no obvious difference in the protein content under treatments with K and phosphorus(P), while the protein content under the treatment of M was slightly lower than the treatment of NPK. In all, M was better than the other fertilizers on quality of sweet potato. Related Articles | Metrics

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Acute toxicity of SDS, Cu2+ pollution and its effect on Na+-K+-ATPase activity in the liver of Bufo gargarizans JIN Ye-fei;ZHU Yao-rong;HAN Li 2010, 22(1):  0-86.  Abstract ( 922 )   PDF (1055KB) ( 1552 )   To investigate the effects of single or combined sodium dodecyl sulfate (SDS) and Cu2+ pollution in Bufo gargarizans, the acute toxicity and joint toxicity tests of SDS, Cu2+ and Na+-K+-ATPase activity in liver under the subacute toxicities of single or combined SDS and Cu2+ were measured by using exposure experiment. The results showed that the LC50 of SDS for 48, 72 and 96 h to Bufo gargarizans were 27.38, 26.45 and 25.57 mg·L-1, respectively, and the LC50 of Cu2+ for 48, 72 and 96 h were 0.475, 0.323 and 0.285 mg·L-1, respectively. The toxicity of Cu2+ was higher than that of SDS. In addition, it also indicated that the joint toxicity of Cu2+ and SDS was synergistic at 48 h but antagonistic at 96 h. The results also indicated that single or combined treatments with Cu2+ and SDS could affect the Na+-K+-ATPase activity in liver of Bufo gargarizans, and the joint effect of the combined pollution on the Na+-K+-ATPase activity was synergistic． Related Articles | Metrics

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Resources survey on symbiotic nodulations and nitrogen-fixing plants in Shanghai greenland ZHANG Jing-wei;YOU Xiang-liang;*;XI Jin-biao;LI Xiu-fen;WANG Kui-ling;ZHANG De-shun;* 2010, 22(1):  0-68.  Abstract ( 1741 )   PDF (832KB) ( 1271 )   Nodulation status and nitrogen fixation were investigated. The host plants collected belong to 5 family 37 genera, totally 83 species. Among of them, 33 genera 71 species were legume plants, the other 4 family 4 genera 12 species were non-leguminous plants. The results showed that 93% of root nodules ranged from 1 to 10 mm at the lateral root of hosts, with spherical or columnar in shape and white or yellowish-brown in color. Nitrogenase activity was from 0 to 10 μmol·g·h-1． Related Articles | Metrics

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Effect of biogas slurry irrigation on soil quality and yield quality in Brassica chinensis WANG Wei-ping;ZHU Feng-xiang;CHEN Xiao-yang;XUE Zhi-yong;HONG Chun-lai;LIU Jian;* 2010, 22(1):  0-76.  Abstract ( 985 )   PDF (872KB) ( 1662 )   Different concentrations of biogas slurry were irrigated in scale raising farms to investigate their effects on the soil environment and quality of Brassica chinensis. Compared with the normal chemical fertilizer (CK), different irrigation amounts of biogas slurry showed no effect on the soil quality. Under equal nitrogen condition, the biogas slurry used as an organic fertilizer with rich nutrient contents instead of nitrogen fertilizer, were helpful to improve quality of Brassica chinensis. Application of biogas slurry with proper dose could not only decrease the wastewater disposal in scale raising farms, but also decrease the application of chemical fertilizer and improve the quality of Brassica chinensis. Related Articles | Metrics

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Phosphorus adsorption property onto synthetic iron oxides under different pH conditions HAN Wei;LIANG Cheng-hua;*;DU Li-yu;LIU Li;WU Yu-mei;AN Ning 2010, 22(1):  0-80.  Abstract ( 1267 )   PDF (827KB) ( 1427 )   In this study, the adsorption of phosphorus onto both crystal and amorphous iron oxides under different pH conditions (i.e. pH=5, 6 and 7) had been investigated using synthetic goethite and ferrihydrite as the materials, respectively. Both Langmuir and Freundlich models were employed to fit the experimental adsorption data to identify the best model. The experimental results showed that these two iron oxides had good adsorption capacity for phosphorus. The modeling results indicated that both models were able to adequately describe the adsorption for both materials, but the Langmuir model was more accurate. Furthermore, through comparison of the equilibrium adsorption quantity (Qmax), the adsorption constants (KL, KF, n) and the maximum buffering capacity (MBC) of two materials, it was found that adsorption capacity of phosphorus onto the ferrihydrite was higher than that of the goethite in each pH condition, and the adsorption capacity for both materials had a considerable reduction with increasing pH whereas the decline trend observed on the ferrihydrite was more pronounced. Related Articles | Metrics

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Investigation on the species of natural enemy insects in peach orchard in Lishui WU Quan-cong;ZHENG Shi-hua 2010, 22(1):  0-95.  Abstract ( 1646 )   PDF (1258KB) ( 1307 )   The species of natural enemy insects in peach orchards were collected and investigated by methods of absorption detection, catching with a net, manual collection, light trap and so on. The results of indoor identification showed that there were 38 kinds of natural enemy insects in peach orchards in Lishui City in southwest of Zhejiang Province. These insects belong to 7 orders, 20 families, and the relative abundance was 0.0659 and the predatory natural enemy insects played a dominant role. There were a large number of natural enemy insects in peach orchards under sod culture, among which, 6 orders, 8 families and 6 species showed higher relative abundance above 0.005. The highest relative abundance was observed in Coleptera, Chrysopidae and Chrysopa septempunctata. However, the natural enemy insects in peach orchards with clean tillage were quite fewer. Related Articles | Metrics

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Direction identification of garlic seeds based on image processing YANG Qing-ming;LI Juan-ling*;HE Rui-yin 2010, 22(1):  0-123.  Abstract ( 1494 )   PDF (1153KB) ( 1579 )   According to the shape characteristics of garlic seeds, a model was developed to identify garlic seeds direction based on Visual C++6.0 software, in which the technology of digital image processing such as conversion, division and expansion was used. The results indicated that the direction of most garlic seeds could be greatly identified and the correct recognition rate reached 92%. The method provided a technique foundation for enhancing garlic seeder automatization． Related Articles | Metrics

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Antibody responses of pigs to different types and doses of Hog cholera vaccines MAO Wen-jie;CHEN Ning;QU Jian;TANG Yu-long;FANG Wei-huan;* 2010, 22(1):  0-90.  Abstract ( 1119 )   PDF (827KB) ( 1468 )   Eighty-one piglets, which derived from 9 sows of 3 different farms were divided into 9 groups, 27 subgroups, with 3 piglets for each subgroup. Each group was vaccinated with Hog cholera tissue vaccine (A), cell vaccine (B) or spleen vaccine (C) with three dose levels (1×, 2×or 3×recommended doses). Blood samples were collected from the 25th day after vaccination for serum. Antibodies were tested by E2 protein based on ELISA and IHA. The results showed that spleen vaccine seemed to be better than the other two though there was no significant difference among them. Higher dose (3×recommended dose) generally induced higher antibody response than lower doses. Higher antibody level was observed after the boosting vaccination. Related Articles | Metrics

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Fitness of Bemisia tabaci biotype B on different cotton varieties (line) XU Hong-xing;ZHENG Xu-song;LIU Shu-ping;QIU Xin-mian;LU Zhong-xian;* 2010, 22(1):  0-99.  Abstract ( 1000 )   PDF (899KB) ( 1363 )   The selectivity and fitness of B-biotype of Bemisia tabaci(Gennadius) on different varieties of cotton was studied in lab. The results showed that the number of adult and egg on glandless cotton line ZH05372 was highest, and the ratio was 46.1% and 47.7%, respectively. The lowest was 23.4% and 23.1% on Cikang 1. There was significant difference in the fitness of B-biotype of Bemisia tabaci(Gennadius) on different varieties of cotton, indicating that the longest nymphal stage was found on ZH05372 and the survival rate of nymph was highest, while the verse trend was found on Zhecaimian 2 and Cikang 1. The longevity of adults on glandless cotton was also longer than those on the other two varieties. The fecundity on glandless cotton ZH05372 and Cikang 1 were greatly more than that on Zhecaimian 2. Related Articles | Metrics

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Expression and purification of porcine interferon-alpha in yeast Pichia pasteris ZHANG Cun;YE Wei-cheng;WANG Yi-cheng;YUAN Xiu-fang;LIU Man-wen;ZHANG Yan 2010, 22(1):  0-9.  Abstract ( 1024 )   PDF (1155KB) ( 1514 )   Porcine interferon-alpha（PoIFNα）was expressed and purified to evaluate its functions. The PoIFNα gene，amplified by PCR，was cloned into P. pasteris expression vector pPIC9k which had been digested by SnaBⅠ and EcoRⅠ enzymes. The transfer plasmid pPIC9k/PoIFNα was verified by enzyme digestion and sequencing. The pPIC9k/PoIFNα linearized by SalⅠ was electro-porated into P. paseris strain GS115. The recombinant yeast GS115/PoIFNα with four-copied PoIIFNα genes was isolated on the YPD agar plate with 1 mg·mL-1 G418. The recombinant protein PoIFNα was expressed in the fermentation supernatant at a high level of 136 mg·L-1 after being induced by methanol. rPoIFNα was isolated at a purity of 81% by one step of SP-Sepharose chromatography. As a result，the purified product was verified to be of high cytokine activity by inhibiting the VSV viral cyto-pathogenic effect on MDBK cell culture，which was about 2.27×108 U·mg-1. Related Articles | Metrics

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Cultivable soil bacteria antibiotic resistance induced by oxytetracycline pollution ZHANG Ming-kui;PU Jin-cheng; 2010, 22(1):  0-72.  Abstract ( 1852 )   PDF (961KB) ( 1469 )   Antibiotics enter agricultural soils by the use of animal excrements as fertilizers. However，it is scarcely known how these antibiotics affect the cultivable soil bacteria antibiotic resistance. Therefore，effect of antibiotic oxytetracycline （OTC） pollution on cultivable soil bacteria antibiotic resistance was studied by addition of different dose of OTC in the soil. Percentage of soil bacteria antibiotic resistance was evaluated by comparing cultivable bacteria number in culture medium spiked with or without OTC. The results showed that soil OTC pollution could increase the cultivable soil bacteria，which was related to the additional dose and times of OTC. The cultivable soil bacteria antibiotic resistance was significantly increased when soil was treated once with OTC at the dose of 4 mg·kg-1，or repeatedly treated twice at the dose of 3 mg·kg-1，or repeatedly treated five times at the dose of 2 mg·kg-1. But，no significant increase in the cultivable soil bacteria antibiotic resistance was found when soil was treated with OTC at the dose of 1 mg·kg-1. The results suggested that long-term application of antibiotics-containing animal manure could improve the development of soil bacteria antibiotic resistance. Related Articles | Metrics

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Effects of plastic greenhouse cultivation on photosynthetic characteristics of ‘Cuiguan’ pear WANG Tao;HU Mei-jun;ZHENG Jie;CHAI Ming-liang;CAI Mei-yan 2010, 22(1):  0-44.  Abstract ( 907 )   PDF (1112KB) ( 1502 )   Effects of plastic greenhouse cultivation on photosynthetic characteristics of ‘Cuiguan’ pear were studied. The results showed that there was no significant difference in photosynthetic characteristic of ‘Cuiguan’ pear between the plastic greenhouse cultivation and open field cultivation，and the difference in net photosynthetic rate （Pn） measured at the same time between the two cultivation patterns was related to the leaf age. The light response curves were similar and the light saturation point was about 800 μmol·m-2·s-1 under the both cultivation patterns. The diurnal variations of Pn and transpiration rate（Tr） of the pear leaves showed single-peak curves. The significant positive correlation was found between Pn and leaf temperature（Tleaf） and the significant negative correction was observed between Pn and relative humidity（RH） during the developing growth phase under the plastic greenhouse cultivation. The two most important ways regulating pear growth and development inside plastic greenhouse were keeping warm and reducing the humidity. Furthermore，improving photosynthesis of the whole pear plant was an important technique under plastic greenhouse cultivation. Related Articles | Metrics

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Study on comprehensive high-yield cultivation of purple cultivar from Dioscorea alata Linn. WU Zhi-gang;WEI Yu-huang;TAO Zheng-ming;*;LENG Chun-hong;JIN Jin-hai;WU Jian-ke 2010, 22(1):  0-39.  Abstract ( 1284 )   PDF (959KB) ( 1498 )   To provide some basic information for high-yield cultivation，the influence of cultivation measures on the yield of purple cultivar from Dioscorea alata Linn. was studied by second order orthogonal rotative regression design and random block experiment. The results indicated that the order of influence on yield was the dosage of stock-tuber>planting density>the dosage of fertilizer，and there was significant interaction effects between the dosage of stock-tube and planting density. When the optimal cultivation measures were as follows：the dosage of stock-tuber from 67.60 g·plant-1 to 76.80 g·plant-1，planting density from 22 110 plant·hm-2 to 23 760 plant·hm-2，the dosage of fertilize from 1386 kg·hm-2 to 1 578 kg·hm-2，the yield would be over 28 000 kg·hm-2. Related Articles | Metrics

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Analysis of mating type of Basidispores in Pleurotus eryngii FENG Wei-lin;CAI Wei-ming;*;JIN Qun-li;FAN Li-jun;LIU Xiao-hong 2010, 22(1):  0-104.  Abstract ( 1368 )   PDF (1522KB) ( 1567 )   Using the method of single spore dilution， 72 monocytes were isolated from the microscopic observation. Through the mating between every two groups of single core strains， 3 kinds of typical colony morphylogy were observed and four different mating types of monocyte strains were obtained. Analysis of DNA fingerprinting for those four types of strains was investigated by ISSR molecular marker technology， and 7 pairs of primers were screened out for amplification. The results showed that 56 DNA polymorphism bands were amplified clearly and easily， among which 9 bands were all observed in the four types of strains and 47 polymorphic bands were observed. It was indicated that the single-core strain was identified as four different strains of the mating type. ISSR molecular markers can be used as an effective tool for identification of single-core strain and fingerprint analysis of edible fungus strains. Related Articles | Metrics

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Study on improvement of thawing quality of the frozen channel catfish （Ictalurus punctatus） fillet SONG Li-li;GAO Hai-yan*;GE Lin-mei;FANG Xiang-jun 2010, 22(1):  0-108.  Abstract ( 912 )   PDF (1018KB) ( 1437 )   Effects of salt solutions on thawing quality and protein denaturation of frozen channel catfish （Ictalurus punctatus） fillet after storage at -18℃，-30℃ and -50℃ were studied. The results showed that the fillet soaked in cryoprotectants exhibited lower pH，water loss，K value and total volatile basic nitrogen content （TVB-N），and maintained better thawing quality after frozen storage as compared with the control. In addition，soaking cryoprotectant in the fillet also reduced the TBARS value，alleviated fatty acid oxidation，increased extractable protein nitrogen value and Ca2+-ATPase activity and reduced the protein denaturation. Related Articles | Metrics

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Cluster head authentication scheme for WSNs in farmland information collection system HE Ju;LU Ming-zhou 2010, 22(1):  0-118.  Abstract ( 1356 )   PDF (1150KB) ( 1360 )   Clustered sensor networks has been introduced into WSNs of environmental monitoring system in agriculture production，which has been shown to be increasingly beneficial to increasing system throughput，decreasing system delay，and conserving energy. However，the existing clustered scheme did not provide authentication for cluster head in its setup phase and steady phase. Hence，this study provided BA-LEACH，which was a new security solution for cluster head authentication and could be helpful to enhance the security in clustered sensor networks with comparatively lower energy consumption. Related Articles | Metrics

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Progress of proteomics research in silkworm （Bombyx mori） LIU Pei-gang;LI Wei-guo;WANG Yong-qiang;* 2010, 22(1):  0-129.  Abstract ( 935 )   PDF (1222KB) ( 1360 )   Bombyx mori is the model organism of lepidopteran insects. In recent years，proteomic research of essential organs in silkworm such as embryo，silk gland，hemolymph，midgut has made considerable progress，providing abundance of information for revealing the related phenomenon and characteristics and its formation mechanism，which were reviewed in this paper. Moreover，the future developmental trends and the application prospects of silkworm proteomics were discussed. Related Articles | Metrics

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Assessing pesticide contamination with earthworm biomarkers CHEN Lin-hua;JIANG Ji-hong;YANG Zheng-jian;MA Yun;*;LIU Wei-ping 2010, 22(1):  0-134.  Abstract ( 1689 )   PDF (1119KB) ( 1491 )   Increasing attention was paid to the problem of pesticide pollution by people. Risk assessment of pesticide should not be only based on the chemical analysis of environmental samples，since this method could’t truly distinguish whether the pesticide was harmful to environment. Thus，biological assessment system should be established. Earthworms were widely used to evaluate the pesticide pollution owing to its large number in soil ecosystem and its role as the bottom of food chain. The changes of earthworm biomarkers measured at the molecular or cellular level could be used to discern agricultural chemical pollution at earlier time. Based on the history of biomarker，earthworm could be used as the model organism to evaluate the soil ecological risk of pesticides. According to this characteristic of earthworm，some kinds of earthworm biomarker （such as acetylcholinesterase，antioxidant enzymes，immunocyte and gene expression） were discussed on its assessment of the pesticide contamination level and its action mechanism. Related Articles | Metrics

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Isolation，identification and molecular characterization for F gene of a Newcastle Disease Virus isolated from pigeon in Shandong YUAN Xiao-yuan;XU Shao-jian;QU Xin-ze;ZHANG Yu-xia;QIN Zhuo-ming 2010, 22(1):  0-24.  Abstract ( 2137 )   PDF (1034KB) ( 1745 )   A Newcastle Disease Virus isolate named JN08 was collected from pigeon in Shandong. F gene of the isolate was cloned and sequenced to study its molecular characterization and hereditary variation. JN08 purified by plaque assay could form typical cytopathic effect. The deduced amino acid sequence of F gene showed that the virus shared a velogenic motif 112RRQKRF117 at the cleavage site. Phylogenetic analysis showed that JN08 and most of the viruses derived from pigeon belonged to genotype VI. For F gene of the isolate JN08，the homologies of the deduced amino acid sequences were 89.9%, 89.7%, 90.4%, 89.7%, 91.3% and 92.8% respectively，compared with strains of LaSota（Ⅱ），B1（Ⅱ），Bingham（Ⅱ），TEX-48（Ⅱ），Ulster67（I）and F48E9（Ⅲ）；the homologies of the deduced amino acid sequences were 91.3%，94.6%，94.8%，95.1% and 94.0% for isolates of P1-03，CH98-98, JS1-06，PB01-96 and GZ-07 from host pigeon in China; the homologies of the deduced amino acid sequences were 96.2%，93.8%，98.0%，96.8%，97.1%，96.8%，96.8% and 98.4% respectively for pigeon-origin isolates of Capital3-97，Tigre6-99，1166-02，IT227-97，2736-00，NY-84，TX3503-04 and 248VB-98 overseas. There was a closer genetic relationship between JN08 isolate and foreign epidemic strains that all belonged to the same gene subtype than host pigeon strains in China. Related Articles | Metrics