Abstract: Eighty-one piglets, which derived from 9 sows of 3 different farms were divided into 9 groups, 27 subgroups, with 3 piglets for each subgroup. Each group was vaccinated with Hog cholera tissue vaccine (A), cell vaccine (B) or spleen vaccine (C) with three dose levels (1×, 2×or 3×recommended doses). Blood samples were collected from the 25th day after vaccination for serum. Antibodies were tested by E2 protein based on ELISA and IHA. The results showed that spleen vaccine seemed to be better than the other two though there was no significant difference among them. Higher dose (3×recommended dose) generally induced higher antibody response than lower doses. Higher antibody level was observed after the boosting vaccination.

Key words: Hog cholera vaccine, antibody response, ELISA, indirect hemagglutination test