DAS-ELISA detection and analysis of six kinds of viruses in plantlets regenerated from Huaiyushan high mountain potato shoot-tips

doi:10.3969/j.issn.1004-1524.2017.10.15

›› 2017, Vol. 29 ›› Issue (10): 1699-1705.DOI: 10.3969/j.issn.1004-1524.2017.10.15

• Plant Protection • Previous Articles Next Articles

DAS-ELISA detection and analysis of six kinds of viruses in plantlets regenerated from Huaiyushan high mountain potato shoot-tips

YIN Minghua, LIU Yan, YU Xueting, LI Yuanfang, ZHOU Rong, FAN Yahong, LUO Yujie, WAN Xiaoxia

College of Life Sciences, Shangrao Normal University, Shangrao 334001, China

Received:2017-04-01 Online:2017-10-20 Published:2017-12-05

Abstract

Abstract: In order to determine the virus species and screen out its virus-free plantlets, 6 viruses (potato leafroll virus, potato virus Y, potato virus X, potato virus S, potato virus M and potato virus A) in plantlets regenerated from shoot-tips of Huaiyushan high mountain potato were detected and analyzed by DAS-ELISA, and the efficiency of different shoot tip detoxification methods were compared. The results showed that there were 6 viruses including potato leafroll virus, potato virus Y, potato virus X, potato virus S, potato virus M and potato virus A in Huaiyushan high mountain potato. Two methods such as “simple shoot tip culture” and “shoot tip culture→thermotherapy→shoot tip culture” can only remove parts of the viruses, while “shoot tip culture→thermotherapy→shoot tip culture→thermotherapy→shoot tip culture” could remove all 6 viruses. In this study, we successfully obtained the virus-free plantlets of Huaiyushan high mountain potato, of which No. 5-1-2 and No. 6-4-1 had better virus-free effect and No. 5-3-2 and No. 6-2-2 had the best virus-free effect. The results will help to provide technical support and theoretical basis for virus-free plantlet industrial production of Huaiyushan high mountain potato and its industrialization development under the background of the fourth major staple food.

Key words: Huaiyushan high mountain potato, plantlets regenerated from shoot-tips, virus, DAS-ELISA

CLC Number:

S532

YIN Minghua, LIU Yan, YU Xueting, LI Yuanfang, ZHOU Rong, FAN Yahong, LUO Yujie, WAN Xiaoxia. DAS-ELISA detection and analysis of six kinds of viruses in plantlets regenerated from Huaiyushan high mountain potato shoot-tips[J]. , 2017, 29(10): 1699-1705.

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DAS-ELISA detection and analysis of six kinds of viruses in plantlets regenerated from Huaiyushan high mountain potato shoot-tips

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