Abstract: Using the method of single spore dilution， 72 monocytes were isolated from the microscopic observation. Through the mating between every two groups of single core strains， 3 kinds of typical colony morphylogy were observed and four different mating types of monocyte strains were obtained. Analysis of DNA fingerprinting for those four types of strains was investigated by ISSR molecular marker technology， and 7 pairs of primers were screened out for amplification. The results showed that 56 DNA polymorphism bands were amplified clearly and easily， among which 9 bands were all observed in the four types of strains and 47 polymorphic bands were observed. It was indicated that the single-core strain was identified as four different strains of the mating type. ISSR molecular markers can be used as an effective tool for identification of single-core strain and fingerprint analysis of edible fungus strains.

Key words: Pleurotus eryngii, mating type, colony morphology, ISSR