Abstract: According to the conserved gene sequence of Duck Hepatitis Virus I(DHV I) in GenBank, one pair of the specific primers were designed. A rapid diagnostic technique of RT-PCR was established by optimizing the reaction condition. The DNA segments amplified were 632 bp. The experiments had proved that RT-PCR possessed a high specificity. And the sensitivity test results indicated that the RT-PCR was more sensitive than the conventional method. Detection of field samples from viral diseased ducks also confirmed that the method of RT-PCR could effectively identify the infection of DHV．

Key words: Duck Hepatitis Virus I, RT-PCR, diagnosis