Abstract: The relationship of PEPC between Brassica napus and Arabidopsis thaliana was analyzed in order to know the background and the distribution expression of PEPC gene in Brassica napus. Meanwhile, probes were designed according to four PEPC genes of Arabidopsis thaliana. The Southern blot results showed that the number of PEPC genes in Brassica napus was certainly more than four. The RT-PCR analysis was carried out to investigate the expression of PEPC gene in seeds at different time. It was indicated that PEPC may have a certain role in accumulation of lipids and storage of proteins or other substances during the seed maturation process．

Key words: phosphoenolpyruate carboxylase (PEPC), Brassica napus, Arabidopsis thaliana