Archive

25 May 2010, Volume 22 Issue 3 Previous Issue    Next Issue
---
论文


For Selected:

Download Citations EndNote Ris BibTeX

Toggle Thumbnails

论文

Select

Cytochrome P450 oxidase activity determination and induced-transcription of CYP9 family genes in Bombyx mandarina ZHAO Hua-qiang;SONG Li-li;LI Bing;SHEN Wei-de;* 2010, 22(3):  0-291.  Abstract ( 1340 )   PDF (1044KB) ( 2506 )   Enzyme linked immunosorbent assay was used to determine the activity of PNOD in the midgut and fat body of Bombyx mandarina under cypermethrin treatment. The results showed that after 24 h induction by cypermethrin, the PNOD activity of fat body and midgut increased by 18.7% and 12.2%, respectively. According to the sequence of CYP9A20(GenBank No. FJ378716), CYP9A21(GenBank No. FJ265741), CYP9A22(GenBank No. EF535806) and CYP9G3 gene segments of Bombyx mandarina, the specific primers were designed. The expression levels of CYP9 family genes of Bombyx mandarina induced by cypermethrin were determined by semi-quantitive RT-PCR. The results indicated that after 24 h induction, the relative mRNA level of CYP9A21 in midgut was 2.1 times of the control, and the relative mRNA levels of CYP9A20, CYP9A21, CYP9G3 in fat body were 1.9, 3.5 and 1.4 times of the control, respectively. The over expression of these cypermethrin P450 genes might enhance the oxidation and detoxification capacity of Bombyx mandarina to cypermethrin． Related Articles | Metrics

Select

Analysis of Brassica napus phosphoenolpyruate carboxylase (PEPC) WANG Xiao-fu;LIU Ren-hu;CHEN Xiao-yun;WANG Fu-lin;GUAN Jing-jing;CHEN Jin-qing;* 2010, 22(3):  0-280.  Abstract ( 984 )   PDF (1198KB) ( 1288 )   The relationship of PEPC between Brassica napus and Arabidopsis thaliana was analyzed in order to know the background and the distribution expression of PEPC gene in Brassica napus. Meanwhile, probes were designed according to four PEPC genes of Arabidopsis thaliana. The Southern blot results showed that the number of PEPC genes in Brassica napus was certainly more than four. The RT-PCR analysis was carried out to investigate the expression of PEPC gene in seeds at different time. It was indicated that PEPC may have a certain role in accumulation of lipids and storage of proteins or other substances during the seed maturation process． Related Articles | Metrics

Select

Preliminary study on the function of rice orthologs of the two genes, ARP6 and SWC6 encoding the subunits of Arabidospsis SWR1 complex XU Yong-han;DENG Min-juan;BAO Lie-ming;PENG Jian-fei;HU Zhang-hua 2010, 22(3):  0-275.  Abstract ( 1612 )   PDF (2558KB) ( 1378 )   In this study, OsARP6 and OsSWC6 in rice, the orthologs of the two genes ARP6 and SWC6 encoding the subunits of Arabidopsis SWR1 complex, respectively, were cloned via RT-PCR and their expression pattern was analyzed. We constructed their expression vectors and then used the vectors to transform Arabidopsis mutants arp6 and swc6, respectively. In the transformants, mutated traits had been rescued by the ectopic expression of OsARP6 or OsSWC6, demonstrating the high conversation of these homologue in function and construction existed between the rice and Arabidopsis. These results provided valuable evidence that there would exist the SWR1 complex in rice, which was of importance for unveiling its molecular mechanism of regulating gene expression． Related Articles | Metrics

Select

Composition and content of glucosinolates in leaves and edible roots of radish YANG Li-juan;ZHOU Sheng-jun;MAO Wei-hai;YUAN Yi;CHEN Xin-juan;* 2010, 22(3):  0-316.  Abstract ( 2390 )   PDF (1015KB) ( 1440 )   The objective of this study was to evaluate the composition and content of GSs in leaves and edible roots of three local radish varieties by high-performance liquid chromatography. The results showed that the composition of GSs in leaves were the same as those in roots and included six aliphatic GSs and three indolyl GSs. However, there were significant differences in the content and relative content of GSs among varieties and organs. In editable roots, the total GSs were 22.59, 12.44 and 27.94 mg·g-1DM and the relative content of total aliphatic GSs were above 95% in three radish varieties, respectively. The content of total GSs and total aliphatic GSs in edible roots were 2.6-4.9 and 2.8-6.0 times higher than those in leaves of three varieties, respectively. The content of total indolyl GSs in edible roots of ‘Chuzhou xiaowuying' was 1.7 times higher than that in leaves, while in ‘Guling xialuobo' and ‘Yunnan bailuobo'the content of total indolyl GSs in editable roots were only 0.37 times and 0.77 times as much as those in leaves. The relative contents of the major GS of 4-methylthio-3-butenyl GS were accounted for 90.43%-93.92% in edible roots and 62.42%-74.05% in leaves. These results suggested that the cruciferous vegetable of radish was rich in GSs and there were significant variations of the content of GSs in different genotypes and organs． Related Articles | Metrics

Select

Isolation and identification of Fusarium species from wilted bitter gourd YANG Ye;ZHOU Qin;JI Hao;WEI Feng;XU Xiao-bo;JIANG Dong-hua* 2010, 22(3):  0-357.  Abstract ( 885 )   PDF (1268KB) ( 1412 )   Twenty-three isolates of Fusarium species were obtained from 10 bitter gourd plants with wilt symptom in Jinhua City using the method of tissue isolation. Based on the culture features, morphological characteristics and ITS sequence, the pathogen of bitter gourd wilt in Jinhua was identified as Fusarium oxysporum. Through pathogenicity and host biotype identification test, the results showed that Fusarium oxysporum was proved to be highly virulent to bitter gourd and less or no virulent to other tested cucurbits. Thus, the wilted bitter gourd was caused by Fusarium oxysporum f. sp. momodicae Sun & Huang． Related Articles | Metrics

Select

The coordinated regulation of nutrients in intensive paddy field DING Neng-fei;GUO Bin;LIN Yi-cheng;LIU Chen;FU Qing-lin;*;LI Jian-qiang 2010, 22(3):  0-353.  Abstract ( 874 )   PDF (911KB) ( 1378 )   In the paddy fields of Southeast China, the non-equilibrium and superfluous inputs of nutrients resulted in several environmental problems such as soil quality degradation, water eutrophication and so on. In this study, the effects of different fertilizer levels on the SPAD values of rice leaves, rice yield, nutrient content and nutrient use efficiency in intensive paddy field were investigated. The results showed that the SPAD values of rice leaves increased with the increasing levels of nitrogen, and the SPAD values showed significant difference in different growth stages as tillering stage＞heading stage＞filling stage. The yield of rice varied significantly under different levels of N and K fertilization, and the optimal N and K fertilizer levels were 225 and 180 kg·hm-2 respectively, but the rice yield did not significantly increase under the application of phosphate fertilizer, which might be due to the higher level of available P in the paddy field． Related Articles | Metrics

Select

Processing technology of instant meatball from chicken bone paste CHEN Li-hong;*;XIAO Chao-geng;TANG Hong-gang;JIA Zhen 2010, 22(3):  0-361.  Abstract ( 1358 )   PDF (956KB) ( 1417 )   In this paper, the effects of different addition amount of chicken meat, bone paste, fat and potato starch content on the quality of meatball were compared by measuring sensory score, and the processing technology of instant meatball from chicken bone paste was established. The results obtained by orthogonal test showed that the optimal content of raw materials were: chicken meat 75%, bone paste 5%, fat 5% and potato starch 10%. The resultant product prepared under optimal conditions was affluent in nutrients and contained good elasticity and flavor. Related Articles | Metrics

Select

Drying properties and quality of Chinese bayberry fruit CAO Xue-dan;QI Xing-jiang;FANG Xiu-gui;ZHAO Kai 2010, 22(3):  0-369.  Abstract ( 1082 )   PDF (920KB) ( 1595 )   The drying characteristics of Chinese bayberry fruit at different hot air drying temperatures were investigated, and the moisture content of the fruit could be predicted by establishing the mathematical models. The results showed that the drying rate of Chinese bayberry fruit declined mostly during the drying process without a constant rate period. The drying characteristics could be well described and predicted by Page model MR=exp(－KtN). The results could provide technical bases for the control of drying technology. Different temperatures of hot air showed no significant effect on vitamin C and total flavonoids content, but significantly affected total sugar, acid and anthocyanin content. Related Articles | Metrics

Select

A two-dimensional electrophoresis protocol suitable for proteomic study of stem in Zizania latifolia Turcz． LIU Qian;RUAN Song-lin;YU Xiao-ping;MA Hua-sheng;YE Zi-hong;* 2010, 22(3):  0-286.  Abstract ( 935 )   PDF (1401KB) ( 1371 )   In search of a method that was adapt to two-dimensional electrophoresis of Zizania latifolia stem, we compared different methods of protein extraction. Optimized conditions for 2-DE were carried out. The results showed that the protein sample of higher concentration and better quality could be acquired if we made proper changes in extraction of TCA-acetone method. In the process, 10% PVP was added in grinding, the centrifugation time was extended to 20 min and the number of precipitation time increased to 4 or 5, meanwhile, lysate was also condensed. We also decreased errors in protein assaying. During the optimization of 2-DE, changes of sample quantity, process of isoelectro focusing(IEF)and the method of staining became important factors. Compared with coomassie brilliant blue G-250 staining, silver ammonia staining detected much more spots. All of the efforts got a better result with more spots, less streak, clearer background and more unconspicuous tailing． Related Articles | Metrics

Select

Influencing factors of polyunsaturated fatty acids (PUFA) hydrogenation in cattle rumen CHEN Yan-le;YE Zi-hong;JIANG Lin-shu;* 2010, 22(3):  0-297.  Abstract ( 1917 )   PDF (926KB) ( 1270 )   The method of rumen fermentation in vitro was used to study the effect of various supplementary feeds, rumen micro-organisms, incubation time on cattle rumen hydrogenation. The results showed that 64.20% variation of NH3-N was affected by incubation time, which was the main effect of variation of NH3-N; various concentrate supplement, rumen micro-organisms and incubation time had significantly different effects on various VFA components; the interaction between incubation time and rumen micro-organisms affected propanoic acid, isobutyric acid, butyric acid significantly (variances of interaction effect were 43.32**,1.56** and 2.87**, respectively). Acetic acid, isovaleric acid and valeric acid were free from the impaction of rumen micro-organisms, incubation time and different supplement. The highest concentration of NH3-N (19.49 mg·mL-1) was observed when extruded soybean was used as supplement. Different supplement, incubation time and interaction between them significantly affected the concentration of stearic acid (totally 77.76% variation was attributed to these three components). The remarkable influence on the content of stearic acid was produced only at specific time in rumen microflora. Concentration of linoleic acid (18∶2) was impacted only by different rumen microflora. Different material, incubation time and interaction in rumen microflora significantly affected the concentration of trans-oleic acid (18∶1) and linolenic acid (18∶3). Related Articles | Metrics

Select

Effects of bursin on avian influenza (H9N2) oil adjuvant inactivated vaccine XU Jia-rong;CHEN Wen;LI Zhen-xing;SHANG Yue-li;XU Jun-cai 2010, 22(3):  0-301.  Abstract ( 893 )   PDF (900KB) ( 1386 )   In order to determine the immune regulatory effect of bursin on avian influenza (H9N2) vaccine, two hundred 13-day-old chicken were randomly divided into four groups. One was for the control, the others for vaccine, vaccine plus bursin and bursin treatments respectively. The change of average index of bursa of fabricius and spleen and serum antibody titer in chicken were examined by means of hemagglutination inhibition test every 7 days from the 13th day after treatment. The results showed that bursin could enhance chicken serum antibody titer on avian influenza (H9N2) oil adjuvant inactivated vaccine and develop bursa of fabricius significantly. Bursin also could prolong the maintenance of high serum antibody titer in chicken. It was suggested that bursin could improve immune effects of avian influenza (H9N2) oil adjuvant inactivated vaccine in chicken． Related Articles | Metrics

Select

Protein expression profiling of soluble protein of male and female eggs in the period of ji3 in Bombyx mori variety ‘W-1’ XIE Zai-dong;LI Bing;WANG Ju-mei;XU Ya-xiang;SHEN Wei-de;* 2010, 22(3):  0-307.  Abstract ( 905 )   PDF (1074KB) ( 1251 )   To investigate the differential expression of soluble protein components, the soluble protein components of male and female eggs in the period of ji3 in Bombyx mori variety ‘W-1’ were compared by two-dimensional electrophoresis technology. The results showed that 317 and 335 protein spots were detected in soluble proteins of male and female egg respectively. Comparison analysis showed that 273 of the total protein spots were matched between male and female egg, and 44 and 62 specific protein spots were found in male and female egg respectively. The results indicated that soluble protein components in the period of ji3 in silkworm were different between the male and female. The characteristic protein spots were identified by MALDI-TOF MS. The result showed that proteins identified were putative keratin, tropomyosin isoform 5, RNA binding protein encoded by Sqd gene, transgelin, heat shock protein 20.4 and tropomyosin 1, indicating that gender has an important influence on the formation of embryonic organs and tissues． Related Articles | Metrics

Select

Phenolic compounds and its metabolism during winter and spring in young loquat fruit of ‘Ninghaibai’ FENG Jian-jun;CHEN Jun-wei;*;XU Hong-xia;XIE Ming;*;WU Yan-jun;ZHANG Hui-qin 2010, 22(3):  0-320.  Abstract ( 1226 )   PDF (1305KB) ( 1410 )   The total phenolic content and activities of phenylalanine ammonia-lysae (PAL), polyphenol oxidase (PPO), peroxidase (POD) in fruits of ‘Ninghaibai' loquat(Eriobotrya japonica Lindl) cultivated in open air, bagging and greenhouse were measured in winter and spring. The results showed that total phenolic content in fruit cultivated in open air condition was significantly higher than those in bagging and greenhouse conditions in winter and decreased with the coming of spring. However, the total phenolic content in fruit cultivated in bagging and greenhouse conditions almost unchanged between winter and spring. The PAL activities of fruits in all treatments in winter were much higher than those in spring, and the activity in open air was higher than those in bagging and greenhouse. Activity of PPO in fruit of open air was the lowest in winter; the highest was in bagging both in winter and spring. The activity of POD in open air was higher than those in bagging and greenhouse in winter. In spring, the activities of POD decreased. These results suggested that the accumulation and metabolism of phenolic compounds might be related to the resistance of freeze in loquat fruit in winter. Related Articles | Metrics

Select

Effect of different light qualities on seedling growth in rice SUN Qing-li;CHEN Zhi;XU Gang;CHEN Min;CHEN Jian-ping;* 2010, 22(3):  0-325.  Abstract ( 998 )   PDF (981KB) ( 1889 )   Seedling growth of rice under different light qualities including three-band fluorescent lamps(TFL), red light emitting diodes(R-LEDs), blue light emitting diodes(B-LEDs) and white light emitting diodes(W-LEDs) were studied. The results indicated that R-LEDs significantly promoted the stem elongation of rice seedlings. Under B-LEDs, the biomass accumulation rate of aerial part of rice seedlings was the highest, but there was no difference among all the four light qualities(P＜0.05). Furthermore, B-LEDs and W-LEDs were more conducive for chlorophyll synthesis in rice seedlings. The longest root of rice seedlings was observed under TFL. There was no difference in root dry and fresh weight under four light qualities. But the root dry and fresh weight ratio under B-LEDs was significantly lower than that under TFL. As a result, the application of LEDs in rice cultivation was feasible． Related Articles | Metrics

Select

Agronomic traits and rice quality of a partial palea mutant induced from a japonica rice variety ‘Zhejing 22’ ZHAO Ning-chun;YE Sheng-hai;LU Yan-ting;TANG Shao-qing;JIN Qing-sheng;ZHANG Xiao-ming;* 2010, 22(3):  0-329.  Abstract ( 966 )   PDF (1000KB) ( 1291 )   Agronomic traits and rice quality of a partial palea mutant induced by irradiation from a japonica rice variety ‘Zhejing 22’ were studied. The results showed there was no difference in plant height and ear length in comparison to its wild type, but there were significant decreases in per panicle, filled grain number per panicle, 1000-grain weight and second panicle branch, which were about half of the wild type. There was a clear decrease in grain width for mutants and little difference in grain length, and the length-width ratio of ‘Zhejing 22’ was 2/3 of the mutant. Chalk percentage and chalkiness degree of the mutant were good, while brown rice rate, milled rice rate and head rice rate was little different from the wild type. The mutant also showed lower RVA profile characters of PKV (peak viscosity), HPV (hot paste viscosity), CPV (cool paste viscosity), SB (setback), and CS (consistency)than ‘Zhejing 22’, meanwhile, the amylose content of the mutant increased. Related Articles | Metrics

Select

Grain yield difference investigation and reasonable planting density analysis of rice production in Zhejing Province WANG Dan-ying;ZHANG Xiu-fu;*;ZHOU Chang-nan;ZHENG Gen-sheng;ZHANG Gen-xian;XU Xi-hu;JIN Bing-hua;ZHANG Wen-song;CHEN Hong-wei;LI Jin 2010, 22(3):  0-336.  Abstract ( 1523 )   PDF (999KB) ( 1669 )   Rice yield difference widely exists among different cultivars and paddy fields. At present, rice yield potential is hard to breakthrough, so decreasing yield difference and increasing yield of different paddy field is one of the effective ways to increase rice average yield. In this study, in order to analyze the cause of rice yield difference, we investigated the grain yield and 23 typical cultivars, which were planted in 236 different paddy fields in Zhejiang Province, including early-season indica conventional rice, single-season indica hybrid rice, single-season japonica hybrid rice, single-season japonica conventional rice and late- double season indica hybrid rice, and further analyzed influence of planting density on grain yield. The results showed that the coefficient of variation of grains per panicle and effective panicles per plant were larger than that of 1 000 grain weight, indicating they were the main causes of yield difference. Relation analysis indicated that the correlation coefficient of grains per panicle and yield was the largest in yield components and was significant in single-season japonica conventional rice and late-season indica hybrid rice, and rice cultivar with large panicle usually had higher yield than cultivar of little panicles. With the increase of planting density, rice grain yield first increased then decreased. In single-season indica hybrid rice, when rice planted in （1.00-1.20）×104 per 667m2, the frequency of yield larger than 650 kg·667m-2 was higher, when planted in （1.20-1.40）×104 per 667m2, the frequency of yield between 500-650 kg·667m-2 was higher, and fewer paddy field was lower than 500 kg·667m-2, grain yield was relatively stable. In late-season indica hybrid rice, the reasonable planting density was （1.10-1.40）×104 per 667m2． Related Articles | Metrics

Select

Effects of canopy position on spatial variations of nutrients in sandy soils in citrus garden MA Wan-zhu;ZHANG Ming-kui;*;LU Xiao-nan;ZHANG Tian-yu 2010, 22(3):  0-348.  Abstract ( 1226 )   PDF (1527KB) ( 1290 )   To evaluate effects of canopy on accumulation of N, P, Cu and Zn in sandy agricultural soils, the horizontal variations of soil available N, P, Cu and Zn in a 96 m2 plot within a citrus garden in Quzhou City of Zhejiang Province were examined. The spatial distribution of soil nutrients in mature sandy orchard showed obvious heterogeneity. The differences in labile nutrients spatial distributions in the soils were related to the interception of canopy to rainfall, which reduced leaching intensity of the soils under the canopies. The results suggested that it was necessary to consider the variation of available nutrients in citrus garden, when soil samples for chemical analysis were collected and amount of applied fertilizers was recommended. Related Articles | Metrics

Select

Fermentation process of glutathione production by Saccharomyces cerevisiae WD-1 WU Xiang-ting;WU Dan;XIA Wen-shui;XU Zhi-nan 2010, 22(3):  0-365.  Abstract ( 943 )   PDF (1169KB) ( 1300 )   Glutathione is an important kind of biologically active tripeptides. It is widely used as a pharmaceutical compound and has the potential to be used in food additives and cosmetic industry. The condition of glutathione biosynthesis by Saccharomyces cerevisiae WD-1 in shaking flasks was studied. The effect of medium compositions, including the selection and optimization of carbon source and organic nitrogen source, inorganic salt and cysteine on glutathione production was investigated. The optimal medium was determined as follows: glucose 2%, yeast extract 4%, peptone 2%, NaCl 1%, MgSO4·7H2O 0.1%, KH2PO4 0.5%,(NH4)2SO4 0.2%, Cysteine 2 mmol·L-1. Orthogonal experiment was carried out in order to determine the optimal conditions, which were as follows: the inoculum size 5%, agitation rate 240 r·min-1 and the containing liquid 30 mL. In the optimal condition, the glutathione yield achieved 223.22 mg·L-1,36.81% higher than the initial production． Related Articles | Metrics

Select

Design of an end-effector for a multi\|use picking robot JU Hong-ling;JI Chang-ying* 2010, 22(3):  0-373.  Abstract ( 1305 )   PDF (1240KB) ( 3930 )   It is necessary to replace manual working by machinery in fruit picking in our country because of the large planting areas and onerous task of harvesting. The current end-effectors were one-to-one picking, thus a new kind of end-effector of picking robot has been designed, which was suitable for picking two kinds of fruits and showed self\|adaptive ability. This end-effector used three fingers centering gripper jaws for apple and pear harvesting, and had the features of small size, simple operation, safe and reliable, adaptable, low cost and so on. The experiments showed that this end-effector could complete picking tasks well． Related Articles | Metrics

Select

Synthesis of 2-(4-chloro benzyl)-1-(1H-1,2,4-triazole-1-)-2-butanol YANG Zhen-ping;SHENG Wei-jian;JIA Jian-hong;GAO Jian-rong* 2010, 22(3):  0-377.  Abstract ( 841 )   PDF (902KB) ( 1220 )   Triazole alcohols are used widely as disinfectants to prevent and treat the common pathogenic bacteria in plants, such as whip trichobacteria, basidiomycetes, imperfect fungi and so on. 2-(4-chlorine benzyl)-1-(1H-1,2,4-triazole-1-)-2-butylacohol was synthesized by Corey epoxidation from p-chloro benzene acetone, and by condensation with the 1H-1,2,4-triazole. The factors influencing the Corey-Chaykovsky epoxidation were studied and the yield was 97.9%. The optimum technological conditions of condensation were determined through orthogonal experiments. The optimum reaction conditions required that the molar ratio of the epoxide, 1H-1,2,4-triazole, and sodium bicarbonate was 1.0∶1.5∶2.1, the reaction temperature was 130℃, and the reaction time was 5 h. The condensation yield was above 82.0%． Related Articles | Metrics

Select

Safety assessment methods of genetically modified (GM) plants and food derived from GM plants XU Jun-feng;CHEN Xiao-yun;SUN Cai-xia;MIAO Qing-mei 2010, 22(3):  0-383.  Abstract ( 904 )   PDF (919KB) ( 1593 )   Rapid development of genetically modification (GM) industry made GM food appear on the table from the laboratory, thus the safety of GM food received worldwide concerns, which involves trade barriers and human health. In this paper, the methods of safety assessment of the transgenic plants and food derived from transgenic plants were discussed according to the connotation and extension of food safety, and foreign genes, genetic modification and unintended effects were analyzed on the basis of substantial equivalence principle. Framework of food safety assessment of GM\|plants was described systematically, which will be a reference to Chinese GM plants safety evaluation and government supervision． Related Articles | Metrics

Select

Analysis on the factors contributing to agricultural technological progress inZhejiang Province ZHANG She-mei;MAO Xiao-bao;HUANG Wei 2010, 22(3):  0-387.  Abstract ( 1500 )   PDF (1000KB) ( 1412 )   After decomposing the factors contributing to agro-tech progress，the weights of 12 main factors from 3 categories such as production technology，management and policy，and service technology in Zhejiang Province were selected and assessed using the Delphi questionnaire survey. The results showed that production technology played the most important role in technology progress with a contribution of more than 50 percent；improvement in plant and animal varieties were the most important among the 12 main factors；education and training for farmers was a comparatively important factor among the management and service technologies. Based on the evaluation，some suggestions were proposed such as enhancing the research and extension of new varieties and techniques，further improving the agro-mechanization and education and training of farmers，and enhancing the harmonious development of production technologies and management and service technologies. Related Articles | Metrics

Select

Impact of regional economic environment on the competitiveness of apple industry: an empirical study from Shaanxi Province XU Li;ZHAO Kai* 2010, 22(3):  0-392.  Abstract ( 845 )   PDF (918KB) ( 1315 )   In order to study the impact of regional economic environment on the competitiveness of apple industry in Shaanxi Province, Delphi method was used to gain the competitiveness index of apple industry, and then we established multiple regression model for studying the effects of regional economic environment on apple industry competitiveness. The results showed that regional economic environmental factors that affected Shaanxi Provinces apple industry competitiveness were regional economic strength, the capacity of government support to agriculture, the level of foreign capital inflows by order of importance． Related Articles | Metrics

Select

Review on the application technology of artificial miRNA in plants GAO Peng; 2010, 22(3):  0-397.  Abstract ( 909 )   PDF (1070KB) ( 1443 )   miRNAs are negative regulators of gene expression in cell. Artificial miRNAs (amiRNA), designed according to the production and principles of natural miRNA, using one or several specific genes as targets, can inhibit gene expression efficiently and specifically. At present, the technology of amiRNA is widely applied in many research fields, such as regulation of gene expression and antivirus． The recent progress in the function priciple of miRNA, the optimization design of amiRNA, gene construction and the application technology was reviewed. Related Articles | Metrics

Select

Review on application of anti-idiotype antibodies in immunoassay for pesticides and mycotoxins LIU Yuan;LIANG Ying;WANG Yun;HE Jiang;LIU Xian-jin 2010, 22(3):  0-402.  Abstract ( 978 )   PDF (1058KB) ( 1429 )   There are some bottlenecks in the development of immunochemical analysis for pesticides and mycotoxins, such as multi-residue analysis, the cost and availability of standard, and limit of sensitivity. However, the anti-idiotype antibodies (AId) have provided some new approaches to solve these problems. In this review, the principles of anti-idiotype antibodies were introduced and the special application advantages of AId in detection of small molecular contaminate in food were discussed. Current applications, restrictions and prospect of AId in these fields were also exemplified． Related Articles | Metrics

Select

Serological survey for newcastle disease and avian influenza of people with occupational poultry exposure HU Bei-xia;XU Chuan-tian;HUANG Yan-yan;ZHANG Wei;YAN Shi-gan;ZHANG Xiu-mei* 2010, 22(3):  0-310.  Abstract ( 1302 )   PDF (886KB) ( 1331 )   In order to assess the infected risk of newcastle disease virus(NDV) and avian influenza virus(AIV) of workers occupationally exposed to poultry, a survey was conducted among workers in large-scale poultry farms and poultry products processing factory. Serum samples of 21 poultry workers and 20 workers in poultry product processing factory were collected and tested for NDV, H5N1 and H9N2 neutralizing antibodies by microneutralization assay. All participants showed negative for H5N1 and H9N2 neutralizing antibodies. Seven poultry workers and one worker of poultry products processing factory were tested positive for NDV neutralizing antibodies. The results showed that the newcastle disease virus could infect human, and there's little possibility of AIV (H5N1 and H9N2 subtype)transmission among people with occupational poultry exposure. Despite no serological evidence of AIV infection was identified among participants, continued surveilance for AIV in humans and further seroprevalence invesitigations were needed to assess the risk of aviantohuman transmission, in case that AIV continued to circulate and evolve among poultry． Related Articles | Metrics

Select

Analysis of yield characters and its stability in rape regional trial of Zhejiang Province YU Qi-ying;ZHAO Wei-ming 2010, 22(3):  0-340.  Abstract ( 1455 )   PDF (898KB) ( 1322 )   Variance components and the stability of yield characters of rape cultivars or lines tested in Zhejiang rape regional trials in 2008 and 2009 were analyzed with the analysis methods for unbalanced data from regional trial of crop variety. The results showed that the yield was mainly affected by the location and interaction between year and location. The yield of line ‘037-77’ was higher than those of the others but there was no significant difference with that of the control (‘Zheshuang 72’). Except for ‘Zheza 0603’ and ‘Qianza 2630’, which were sensitive to environmental composite indexes, and ‘J6’ and ‘ZY-10’, which showed poor identity, the other cultivars or lines showed similar stability． Related Articles | Metrics

Select

Changes of carbohydrate metabolism within growth and storage process of pumpkin fruit YU Jin-long;XU Li-shan;YU Jin-zhong;CHENG Lin-run;ZHU Pu 2010, 22(3):  0-344.  Abstract ( 1361 )   PDF (934KB) ( 1457 )   The changes of the content of starch, soluble sugar and the activity of amylase were studied in two pumpkin varieties of ‘Ganli’,‘Huali during fruit growth and storage. The results showed that there were great differences in starch accumulation and final content between two pumpkin varieties. The content of starch, soluble sugar and the activity of amylase increased with fruit growth. At different storage temperature (6℃ and 25℃), the content of starch declined and the content of soluble sugar increased, and larger changes was observed at 25℃． Related Articles | Metrics