Abstract: Twenty-three isolates of Fusarium species were obtained from 10 bitter gourd plants with wilt symptom in Jinhua City using the method of tissue isolation. Based on the culture features, morphological characteristics and ITS sequence, the pathogen of bitter gourd wilt in Jinhua was identified as Fusarium oxysporum. Through pathogenicity and host biotype identification test, the results showed that Fusarium oxysporum was proved to be highly virulent to bitter gourd and less or no virulent to other tested cucurbits. Thus, the wilted bitter gourd was caused by Fusarium oxysporum f. sp. momodicae Sun & Huang．

Key words: bitter gourd, Fusarium oxysporum, pathogenicity, host biotype identification test