›› 2010, Vol. 22 ›› Issue (3): 0-275.

• 论文 •    

Preliminary study on the function of rice orthologs of the two genes, ARP6 and SWC6 encoding the subunits of Arabidospsis SWR1 complex

XU Yong-han;DENG Min-juan;BAO Lie-ming;PENG Jian-fei;HU Zhang-hua   

  1. 1Key Laboratory of Genetic Engineering of Plant Metabolism of Zhejiang Province, Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China; 2College of Bio-Safety Science and Technology, Hunan Agricultural University, Changsha 410128, China
  • Received:1900-01-01 Revised:1900-01-01 Online:2010-05-25 Published:2010-05-25

Abstract: In this study, OsARP6 and OsSWC6 in rice, the orthologs of the two genes ARP6 and SWC6 encoding the subunits of Arabidopsis SWR1 complex, respectively, were cloned via RT-PCR and their expression pattern was analyzed. We constructed their expression vectors and then used the vectors to transform Arabidopsis mutants arp6 and swc6, respectively. In the transformants, mutated traits had been rescued by the ectopic expression of OsARP6 or OsSWC6, demonstrating the high conversation of these homologue in function and construction existed between the rice and Arabidopsis. These results provided valuable evidence that there would exist the SWR1 complex in rice, which was of importance for unveiling its molecular mechanism of regulating gene expression.

Key words: rice, SWR1 complex, subunit, Arabidopsis, complementation