Abstract: According to the sequences of duck hepatitis virus type Ⅰ （DHV-I） published in GenBank,a pair of primers were designed and synthesized. A specific 699 bp fragment was amplified and successfully sequenced from RNA templates of DHV -I Z10 strain. Alignment results showed that the homologies were higher than 90% compared with published sequence of DHV-I in GenBank. The amplified results of newcastle disease virus （NDV）,infectious bursal disease virus （IBDV）,duck plague virus （DPV） and goose paramyxo viridae （GPV） were negative. The RT-PCR method could detect the duck hepatitis virus with only 24 pg RNA,whieh was specific ,rapid and sensitive. The results indicated that the method could be used for the rapid diagnosis of DHV-I isolates.

Key words: duck hepatitis virus type Ⅰ（DHV-I）, RT-PCR, deteetion