转Pal基因拟南芥对根际土壤细菌群落的影响

doi:10.3969/j.issn.1004-1524.2016.12.16

浙江农业学报 ›› 2016, Vol. 28 ›› Issue (12): 2068-2075.DOI: 10.3969/j.issn.1004-1524.2016.12.16

转Pal基因拟南芥对根际土壤细菌群落的影响

刘丹, 吴凤芝^*

东北农业大学园艺学院,黑龙江哈尔滨 150030

收稿日期:2016-05-12 出版日期:2016-12-15 发布日期:2017-01-05
通讯作者: 吴凤芝,E-mail: fzwu2006@aliyun.com
作者简介:刘丹(1984—),女,黑龙江牡丹江人,博士研究生,从事植物分子生物学研究。E-mail:mdjliudan@126.com
基金资助:
国家自然科学基金项目(31172002)

Effect of phenylalanine ammonialyase transgenic Arabidopsis thaliana on bacterial community in rhizosphere soil

LIU Dan, WU Feng-zhi^*

Department of Horticulture, Northeast Agricultural University, Harbin 150030, China

Received:2016-05-12 Online:2016-12-15 Published:2017-01-05

摘要/Abstract

摘要： 利用 Miseq测序技术研究了转苯丙氨酸解氨酶基因(Pal)拟南芥对其根际土壤细菌群落结构和多样性的影响。结果表明,转Pal基因拟南芥土壤细菌Chao指数显著高于突变体和野生型;从Shannon和Simpson指数来看,转Pal基因拟南芥根际土壤细菌的群落多样性显著高于野生型和突变体;转Pal基因处理与突变体相比,在门水平上变形菌门(Actinobacteria)的相对丰度增加,在纲水平上α-变形菌纲(Alphaproteobacteria)的相对丰度增加;转Pal基因拟南芥起作用的主要类群为变形菌门(Proteobacteria)、蓝藻菌门(Cyanobacteria)、绿弯菌门(Chloroflexi)、α-变形菌纲(Alphaproteobacteria)、δ-变形菌纲(Deltaproteobacteria)、粘球菌目(Myxococcales)等。转基因拟南芥根系可能促进或抑制具有某些特定生理特征的根际微生物种群,从而改变了土壤细菌群落的结构和多样性。

关键词: 转基因拟南芥, 细菌, 群落结构, 多样性

Abstract: The effect of phenylalanine ammonialyase (Pal) transgenic Arabidopsis thaliana on the bacterial structure and diversity in rhizosphere soil was studied by Miseq method. The results showed that the Chao index for the bacterial communities was calculated, and was significantly higher in the rhizospheres collected from the AcPal⁺ transgenic Arabidopsis than the ones collected from the Pal^- mutant Arabidopsis or wild type Arabidopsis. The Shannon diversity index and Simpson index for the bacterial communities were calculated, and both community diversity were significantly higher in the rhizospheres collected from the AcPal⁺ transgenic Arabidopsis than the ones collected from the Pal^- mutant Arabidopsis or wild type Arabidopsis. The Actinobacteria relative abundances were increased at phylum levels, as well as Alphaproteobacteria relative abundances at class levels from the AcPal⁺ transgenic Arabidopsis than those from the Pal^- mutant Arabidopsis. Major microbial groups of AcPal⁺ transgenic Arabidopsis are Proteobacteria, Cyanobacteria, Chloroflexi, Alphaproteobacteria, Deltaproteobacteria, Myxococcales etc. It indicated that the transgenic Arabidopsis roots might promote or inhibit the development of certain physiological characteristics of the bacterial community, and hence a change in the structure and diversity of the soil microbial communities.

Key words: transgenic Arabidopsis thaliana, bacteria, community structure, diversity

中图分类号:

Q785

刘丹, 吴凤芝. 转Pal基因拟南芥对根际土壤细菌群落的影响[J]. 浙江农业学报, 2016, 28(12): 2068-2075.

LIU Dan, WU Feng-zhi. Effect of phenylalanine ammonialyase transgenic Arabidopsis thaliana on bacterial community in rhizosphere soil[J]. , 2016, 28(12): 2068-2075.

参考文献

[1] PELLEGRINI L, ROHFRITSCH O, FRITIG B, et al. Phenylalanine ammonialyase in tobacco: molecular cloning and gene expression during the hypersensitive reaction to tobacco mosaic virus and the response to a fungal elicitor[J]. Plant Physiology , 1994, 106(3): 877-886.
[2] DIXON R A, PAIVA N L. Stress-induced phenylpropanoid metabolism[J]. Plant Cell , 1995,7(7): 1085-1097.
[3] LIU R R, XU S H, LI J L, et al. Expression profile of a PAL gene from Astragalus membranaceus var. Mongholicus and its crucial role in flux into flavonoid biosynthesis[J]. Plant Cell Reports , 2006, 25(7): 705-710.
[4] MAHESH V, RAKOTOMALALA J J, VIGNE L L H, et al. Isolation and genetic mapping of a Coffea canephora phenylalanine ammonia-lyase gene (CcPAL1) and its involvement in the accumulation of caffeoyl quinic acids[J]. Plant Cell Reports , 2006,25(9): 986-992.
[5] SINGH K, KUMAR S, GULATI A R A, et al. Phenylalanine ammonia-lyase (PAL) and cinnamate 4-hydroxylase (C4H) and catechins (flavan-3-ols) accumulation in tea[J]. Functional & Integrative Genomics , 2009, 9(1): 125-134.
[6] 王玲平, 周生茂, 戴丹丽,等. 植物酚类物质研究进展[J].浙江农业学报, 2010, 22(5): 696-701.
WANG L P, ZHOU S M, DAI D L, et al. Progress in plant phenolic compounds [J]. Acta Agriculturae Zhejiangensis , 2010, 22(5): 696-701. (in Chinese with English abstract)
[7] 熊君, 王海斌, 方长旬, 等. 不同氮素供应下水稻酚类物质代谢关键酶基因差异表达[J]. 植物生理与分子生物学学报, 2007, 33(5): 387-394.
XIONG J, WANG H B, FANG C X, et al. The differential expression of the genes of the key enzymes involved in phenolic compound metabolism in rice ( Oryza sativa L. ) under different nitrogen supply[J]. Journal of Plant Physiology and Molecular Biology , 2007, 33(5): 387-394. (in Chinese with English abstract)
[8] FANG C, ZHUANG Y, XU T, et al. Changes in rice allelopathy and rhizosphere microflora by inhibiting rice phenylalanine ammonia-lyase gene expression[J]. Journal of Chemical Ecology , 2013, 39(2): 204-212.
[9] WARDLE D A, BARDGETT R D, KLIRONOMOS J N, et al. Ecological linkages between aboveground and belowground biota[J]. Science , 2004, 304(5677): 1629-1633.
[10] SAXENA D, FLORES S, STOTZKY G. Bt toxin is released in root exudates from 12 transgenic corn hybrids representing three transformation events[J]. Soil Biology & Biochemistry , 2002, 34(1): 133-137.
[11] 赵云丽, 李刚, 修伟明,等. 非抗虫转基因棉花对土壤细菌群落多样性的影响[J]. 农业环境科学学报, 2015, 34(4):716-721.
ZHAO Y L, LI G, XIU W M, et al. Effects of insect non-resistant transgenic cottons on bacterial community diversity in soil[J]. Journal of Agro-Environment Science , 2015, 34(4):716-721. (in Chinese with English abstract)
[12] 李海峰, 刘岩, 康颖,等. 转基因小黑杨对土壤微生物群落结构的影响[J]. 南京林业大学学报(自然科学版), 2014, 38(2):75-80.
LI H F, LIU Y, KANG Y, et al. Influence of transgenic Populus simonii×P. nigra on soil microbial community[J]. Journal of Nanjing Forestry University ( Natural Sciences Edition ), 2014, 38(2):75-80. (in Chinese with English abstract)
[13] WATRUD L S, SEIDLER R J. Nontarget ecological effects of plant, microbial, and chemical introductions to terrestrial systems [M] //HUANG P M. Soil chemistry and ecosystem health. Madison: Soil Science Society of America, Inc,1998: 313-340.
[14] 林文雄. 化感水稻抑草作用的根际生物学特性与研究展望[J]. 作物学报, 2013, 39(6): 951-960.
LIN W X. Rhizobiological properties of allelopathic rice in suppression of weeds and its research prospect[J]. Acta Agronomica Sinica , 2013, 39(6): 951-960. (in Chinese with English abstract)
[15] CIPOLLINI D, RIGSBY C M, BARTO E K. Microbes as targets and mediators of allelopathy in plants[J]. Journal of Chemical Ecology , 2012,38 (6): 714-727.
[16] 王振, 赵廷昌, 刘学敏,等. 转基因作物对土壤微生物多样性影响[J]. 植物保护, 2007, 33(4):15-20.
WANG Z, ZHAO T C, LIU X M, et al. Advances in research on the impacts of transgenic crops on soil microbes[J]. Plant Protection , 2007, 33(4):15-20. (in Chinese with English abstract)
[17] 张美俊, 杨武德. 转 Bt 基因作物毒蛋白对根际土壤生态系统影响研究进展[J]. 山西农业大学学报, 2005, 25 (3): 302-305.
ZHANG M J, YANG W D. Advances in research on effects of transgenic Bt crops on rhizosphric soil ecosystem[J]. Journal of Shanxi Agricultural University , 2005, 25 (3): 302-305. (in Chinese with English abstract)
[18] COELHO M R, DE VOS M, CARNEIRO N P, et al. Diversity of nifH gene pools in the rhizosphere of two cultivars of sorghum ( Sorghum bicolor ) treated with contrasting levels of nitrogen fertilizer[J]. FEMS Microbiology Letters , 2008, 279 (1): 15-22.
[19] WIDMER F, SHAFFER B T, PORTEOUS L A, et al. Analysis of nifH gene pool complexity in soil and litter at a Douglas fir forest site in the Oregon Cascade mountain range[J]. Applied and Environmental Microbiology , 1999, 65(2): 374-380.
[20] ADHIKARI T B, JOSEPH C M, YANG G, et al. Evaluation of bacteria isolated from rice for plant growth promotion and biological control of seedling disease of rice[J]. Canadian Journal of Microbiolgy , 2001,47(10): 916-924.
[21] 王海斌,何海斌,曾聪明,等. 低磷胁迫下不同品种水稻秧苗生长的分子生理特性[J]. 应用与环境生物学报, 2008,14(5): 593-598.
WANG H B, HE H B, ZENG C M, et al. Molecular physiological properties of different rice accessions mediated by different phosphorus supplies at seedling stage[J]. Chinese Journal of Applied Environmental Biology , 2008, 14(5): 593-598. (in Chinese with English abstract)
[22] ALEXEI M, PETER L, INGE B, et al. Pyrolysis-field ionization mass spectrometry of genetically modified plants on soil-organisms[J]. Environmental Biosafety Research , 2006, 5: 37-46.
[23] MACGREGOR A N, TURNER M A. Soil effects of transgenic agriculture: biological processes and ecological consequences[J]. New Zealand Soil News , 2000, 48 (6): 166-169.
[24] KREMER R J, MEANS N E. Glyphosate affects soybean root exudation and rhizosphere microorganisms [J]. International Journal of Environmental Analytical Chemistry , 2005, 85 (15):1165-1174.

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转Pal基因拟南芥对根际土壤细菌群落的影响

Effect of phenylalanine ammonialyase transgenic Arabidopsis thaliana on bacterial community in rhizosphere soil

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