上饶早梨主栽品种病毒种类分析及其茎尖脱毒技术效率比较

doi:10.3969/j.issn.1004-1524.2017.01.13

浙江农业学报 ›› 2017, Vol. 29 ›› Issue (1): 89-100.DOI: 10.3969/j.issn.1004-1524.2017.01.13

上饶早梨主栽品种病毒种类分析及其茎尖脱毒技术效率比较

尹明华¹, 周宇瑶², 杨星鹏¹, 徐玉琴¹, 刘郑英¹, 舒荣建¹, 魏志敏¹, 夏华炎¹, 洪森荣^{1, *}

1.上饶师范学院生命科学学院,江西上饶334001;
2.北京中医药大学人文学院,北京 100029

收稿日期:2016-05-05 出版日期:2017-01-15 发布日期:2017-02-23
通讯作者: 洪森荣,E-mail:hongsenrong@163.com
作者简介:尹明华(1973—),女,江西永新人,硕士,副教授,主要从事植物生物技术方面的研究。E-mail:yinminghua04@163.com
基金资助:
2014年度江西省高等学校科技落地计划产学研合作项目(KJLD14086); 上饶师范学院科研基金资助项目(201410); 2016年中央财政支持地方高校发展专项资金项目——赣东北生态农业技术与应用研究基地子项目

Analysis of virus species in main cultivars of early pear in Shangrao and the efficiency of their shoot tip virus-free techniques

YIN Minghua¹, Zhou Yuyao², YANG Xingpeng¹, XU Yuqin¹, LIU Zhengying¹, SHU Rongjian¹, WEI Zhimin¹, Xia Huayan¹, Hong Senrong^{1, *}

1. College of Life Sciences, Shangrao Normal University, Shangrao 334001, China;
2. College of Humanities, Beijing University of Chinese Medicine, Beijing 100029, China

Received:2016-05-05 Online:2017-01-15 Published:2017-02-23

摘要/Abstract

摘要： 对上饶早梨3个主栽品种的病毒种类进行分析,并对其不同茎尖脱毒技术的效率进行比较。结果表明,花厅六月雪和田墩六月雪没有检测到苹果茎痘病毒(ASPV),花厅黄皮消检测到ASPV,田墩六月雪、花厅六月雪和花厅黄皮消均检测到苹果茎沟病毒(ASGV)和苹果锈果类病毒(ASSVd),但均未检测到苹果褪绿叶斑病毒(ACLSV)和苹果花叶病毒(ApMV)。对于上饶早梨3个主栽品种来说,超低温疗法的脱毒效率显著高于常规茎尖培养,在3种超低温疗法脱毒技术中,包埋玻璃化法超低温疗法的脱毒效率最高。SSR荧光标记毛细管电泳检测表明,常规茎尖培养和3种超低温疗法脱毒技术均能保证上饶早梨3个主栽品种的遗传稳定性。该研究有助于上饶早梨种苗病毒病害的检测鉴定和实时监控检疫,同时也可为建立上饶早梨种质资源超低温库和生产无毒苗提供一定的技术支撑。

关键词: 上饶早梨, 主栽品种, 病毒种类, 茎尖, 超低温疗法, 脱毒

Abstract: Virus species in 3 main cultivars of early pear in Shangrao were analyzed and the efficiency of their shoot tip virus-free techniques was compared. The results showed that Apple stem pitting virus (ASPV) was not detected in Huating Liuyuexue and Tiandun Liuyuexue, but it was detected in Huating Huangpixiao. Apple stem grooving virus(ASGV) and Apple scar skin viroid(ASSVd) were both detected in Huating Liuyuexue, Huating Huangpixiao and Tiandun Liuyuexue, but Apple chlorotic leaf spot virus(ACLSV) and Apple mosaic virus (ApMV) were not detected in them. For 3 main cultivars of early pear in Shangrao, the virus-free efficiency of cryotherapy was significantly higher than that of the conventional shoot tip culture. Among three virus-free techniques by cryotherapy, the virus-free efficiency of cryotherapy by encapsulation-vitrification was the highest. Through SSR fluorescence labeled capillary electrophoresis analysis, the conventional shoot tip culture and three cryotherapy virus-free techniques all could ensure the genetic stability of 3 main cultivars of early pear in Shangrao. The results contributed to the viral disease detection, identification and their real-time monitoring and quarantine of early pear seedling in Shangrao, which also provided some technical support for building the cryopreservation germplasm resources of early pear in Shangrao and its production of virus-free plantlets.

Key words: early pear in Shangrao, main cultivars, virus species, shoot tip, cryotherapy, virus-free

中图分类号:

S661.2

尹明华, 周宇瑶, 杨星鹏, 徐玉琴, 刘郑英, 舒荣建, 魏志敏, 夏华炎, 洪森荣. 上饶早梨主栽品种病毒种类分析及其茎尖脱毒技术效率比较[J]. 浙江农业学报, 2017, 29(1): 89-100.

YIN Minghua, Zhou Yuyao, YANG Xingpeng, XU Yuqin, LIU Zhengying, SHU Rongjian, WEI Zhimin, Xia Huayan, Hong Senrong. Analysis of virus species in main cultivars of early pear in Shangrao and the efficiency of their shoot tip virus-free techniques[J]. , 2017, 29(1): 89-100.

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上饶早梨主栽品种病毒种类分析及其茎尖脱毒技术效率比较

Analysis of virus species in main cultivars of early pear in Shangrao and the efficiency of their shoot tip virus-free techniques

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