台湾柠檬上柑橘溃疡病菌的分离鉴定

doi:10.3969/j.issn.1004-1524.2017.01.14

浙江农业学报 ›› 2017, Vol. 29 ›› Issue (1): 101-105.DOI: 10.3969/j.issn.1004-1524.2017.01.14

台湾柠檬上柑橘溃疡病菌的分离鉴定

陈先锋, 张慧丽^*, 赵雷

宁波出入境检验检疫局,浙江宁波 315012

收稿日期:2016-05-23 出版日期:2017-01-15 发布日期:2017-02-23
通讯作者: 张慧丽,E-mail:zhanghl@nbciq.gov.cn
作者简介:陈先锋(1972—),男,浙江东阳人,博士,研究员,主要从事植物检疫。E-mail:chenxf@nbciq.gov.cn
基金资助:
宁波市科技创新团队(2015c110018)

Isolation and identification of Xanthomonas axonopodis pv. citr on lemon samples from Taiwan

CHEN Xianfeng, ZHANG Huili^*, ZHAO Lei

Ningbo Exit & Entry Inspection and Quarantine Bureau, Ningbo 315012, China

Received:2016-05-23 Online:2017-01-15 Published:2017-02-23

摘要/Abstract

摘要： 从送检台湾柠檬样品上发现疑似柑橘溃疡病病斑,对病原菌进行了分离纯化,并且通过形态学特征观察、致病性测定、特异性引物扩增和gyrB序列比对分析,对其进行了鉴定。结果显示,菌株6503在NA培养基上菌落呈黄色,圆形,全缘,有光泽,微隆起,黏稠;致病性测定结果显示,分离物6503能够导致柑橘叶片产生明显的溃疡病斑;柑橘溃疡病菌种特异性引物Xac01/Xac02从菌株6503的DNA中扩增得到581 bp的特异性条带;基于gyrB序列构建的系统发育树中6503和柑橘溃疡病菌处于同一分支,亲缘关系最近。根据试验结果,菌株6503被鉴定为柑橘溃疡病菌。

关键词: 台湾柠檬, 柑橘溃疡病, gyrB, 致病性, 鉴定

Abstract: A typical symptom of citrus bacterial canker was observed on inspection of lemon samples imported from Taiwan. A strain 6503 was isolated from this sample and was identified by morphological observation, pathogenicity determination, specific-PCR detection and gyrB sequence analysis. The colony morphology of strain 6503 on nutrient agar medium (NA) was yellow, circular, entire, glistening, convex, mucoid. The pathogenicity test demonstrated that strain 6503 was pathogenic to the leaf of citrus. Strain 6503 can be amplified and produced 581 bp target band with specific primer Xac01/Xac02. Strain 6503 and X. axonopodis pv. citri were within the same clade in phylogenetic tree based on gyrB sequences. Based on these results above, strain 6503 was identified as X. axonopodis pv. citri.

Key words: lemon from Taiwan, X. axonopodis pv. citr, gyrB, pathogenicity, identification

中图分类号:

S436.661

陈先锋, 张慧丽, 赵雷. 台湾柠檬上柑橘溃疡病菌的分离鉴定[J]. 浙江农业学报, 2017, 29(1): 101-105.

CHEN Xianfeng, ZHANG Huili, ZHAO Lei. Isolation and identification of Xanthomonas axonopodis pv. citr on lemon samples from Taiwan[J]. , 2017, 29(1): 101-105.

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台湾柠檬上柑橘溃疡病菌的分离鉴定

Isolation and identification of Xanthomonas axonopodis pv. citr on lemon samples from Taiwan

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