铜藻多糖在烟丝中的保润性能、热裂解及其生物安全性分析

doi:10.3969/j.issn.1004-1524.2017.05.21

浙江农业学报 ›› 2017, Vol. 29 ›› Issue (5): 831-839.DOI: 10.3969/j.issn.1004-1524.2017.05.21

铜藻多糖在烟丝中的保润性能、热裂解及其生物安全性分析

黄芳芳¹, 尹洁¹, 严志鹏², 肖卫强¹, 毕一鸣¹, 杨艳芹¹, 周国俊¹, 牛芳芳¹, 杨君^{1, *}

1.浙江中烟工业有限责任公司技术中心,浙江杭州 310024；
2.浙江工业大学海洋学院,浙江杭州 310014

收稿日期:2016-11-16 出版日期:2017-05-20 发布日期:2017-06-06
通讯作者: 杨君,E-mail: yangjun@zjtobacco
作者简介:黄芳芳(1980-),女,浙江平阳人,硕士,高级工程师,研究方向为天然植物提取和烟草化学。E-mail: tinahff@163.com
基金资助:
浙江中烟工业有限责任公司科技项目(ZJZY2014C001); 国家自然科学基金(31301560)

Moisture retentiveness, pyrolysis productions and biological safety of polysaccharides extracts from Sargassum horneri in cut tobacco

HUANG Fangfang¹, YIN Jie¹, YAN Zhipeng², XIAO Weiqiang¹, BI Yiming¹, YANG Yanqin¹, ZHOU Guojun¹, NIU Fangfang¹, YANG Jun^{1, *}

1. China Tobacco Zhejiang Industrial Co., Ltd., Hangzhou 310024, China;
2. Ocean College, Zhejiang University of Technology, Hangzhou 310014, China

Received:2016-11-16 Online:2017-05-20 Published:2017-06-06

摘要/Abstract

摘要： 采用不同的提取分离手段从铜藻(Sargassum horneri)中分别得到铜藻多糖水提物(A)、醇提物(B)、径向流色谱脱蛋白提取物(C)、化学法脱蛋白提取物(D),将其添加于烟丝中,开展物理保润性能和生物安全性测定,同时利用热裂解-气相色谱/质谱联用技术(Py-GC/MS)对4种铜藻多糖提取物进行分析。结果显示:铜藻多糖在高湿或低湿条件下均能有效减缓烟丝水分的变化;铜藻多糖提取物A于6 h内保润性能最佳,提取物D次之;4种铜藻多糖提取物热裂解产物主要为杂环类、酮类和糠醛类;生物安全性评价表明,4种提取物安全无毒;添加了铜藻多糖的卷烟品质总体有所提升,一定程度上改善了吸食的口感,表明铜藻多糖提取物是一种天然安全的保润剂。

关键词: 铜藻多糖, 保润性能, 气相-质谱分析仪, 热裂解, 生物安全性

Abstract: Four kinds of polysaccharide including water extract (A), ethanol extract (B), radial flow-chromatography extract (C), and chemical deproteinization (D) were obtained from Sargassum horneri and added to the cut tobacco. The physical moisturizing property and biological safety were detected. In addition, the pyrolysis products of the four kinds of polysaccharides were analyzed by pyrolysis-gas chromatography/mass spectrometry (Py-GC/MS). The results showed that the polysaccharides from Sargassum horneri could effectively reduce the change of moisture content in tobacco under high humidity or low humidity condition. The moisturizing performance of polysaccharide extract A was the best within 6 h, followed by extract D. The pyrolysis products of the polysaccharide extracts were mainly heterocyclic, ketone and furfural. The tobacco smoke increased with the addition of the extract, and the taste of the smoked tobacco was improved to some extent, which indicated that the polysaccharide extract was a potential natural security protection.

Key words: Sargassum horneri polysaccharide, moisture retentivity, Py-GC/MS, pyrolysis, biological safety

中图分类号:

S572

黄芳芳, 尹洁, 严志鹏, 肖卫强, 毕一鸣, 杨艳芹, 周国俊, 牛芳芳, 杨君. 铜藻多糖在烟丝中的保润性能、热裂解及其生物安全性分析[J]. 浙江农业学报, 2017, 29(5): 831-839.

HUANG Fangfang, YIN Jie, YAN Zhipeng, XIAO Weiqiang, BI Yiming, YANG Yanqin, ZHOU Guojun, NIU Fangfang, YANG Jun. Moisture retentiveness, pyrolysis productions and biological safety of polysaccharides extracts from Sargassum horneri in cut tobacco[J]. , 2017, 29(5): 831-839.

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铜藻多糖在烟丝中的保润性能、热裂解及其生物安全性分析

Moisture retentiveness, pyrolysis productions and biological safety of polysaccharides extracts from Sargassum horneri in cut tobacco

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