蝼蛄虫草的虫体与草体中核苷、虫草酸及氨基酸含量比较

doi:10.3969/j.issn.1004-1524.2017.05.20

浙江农业学报 ›› 2017, Vol. 29 ›› Issue (5): 824-830.DOI: 10.3969/j.issn.1004-1524.2017.05.20

蝼蛄虫草的虫体与草体中核苷、虫草酸及氨基酸含量比较

叶日英¹, 王雅玲^{1, *}, 侯宏伟¹, 张春辉², 孙力军¹, 徐德锋¹

1.广东海洋大学食品科技学院,广东湛江 524088；
2.明大检测认证技术有限公司,广西钦州 535000

收稿日期:2016-12-15 出版日期:2017-05-20 发布日期:2017-06-06
通讯作者: 王雅玲,E-mail: gdouwang@163.com
作者简介:叶日英(1965-),女,广东湛江人,高级实验师,从事食品微生物与食品质量安全研究工作。E-mail: zjyz1303@163.com
基金资助:
广东省科技厅公益研究与能力建设专项资金项目(2014B020204005粤科规财字[2015]72号)

Comparison and analysis of nucleoside, cordycepic acid and amino acid content in insect body and stroma of Cordyceps gryllotalpae

YE Riying¹, WANG Yaling^{1, *}, HOU Hongwei¹, ZHANG Chunhui², SUN Lijun¹, XU Defeng¹

1. College of Food Science and Technology, Guangdong Ocean University, Zhanjiang 524088, China. ;
2. Maindata Inspection and Certification Technology Co. Ltd., Qinzhou 535000, China

Received:2016-12-15 Online:2017-05-20 Published:2017-06-06

摘要/Abstract

摘要： 为了检测蝼蛄虫草虫体与草体中核苷、虫草酸及氨基酸含量,采用超声波提取水溶性成分,建立HPLC检测体系检测核苷,用分光光度计检测虫草酸,氨基酸自动分析仪检测氨基酸。结果表明:核苷检测回收率95.73%~99.00%,出峰时间精密度0.05%~0.14%,峰面积精密度0.12%~0.84%;虫体的尿苷、肌苷、鸟苷、腺苷含量分别为2.86、0.43、2.12、0.17 mg·g^-1,草体的尿苷、肌苷、鸟苷、腺苷含量分别为3.53、0.89、3.51、1.39 mg·g^-1;虫草酸检测回收率98.29%,虫体、草体中虫草酸含量分别是109.64、127.46 mg·g^-1,氨基酸分数分别是56.3和62.8,必需氨基酸指数分别是81.9和115.4。草体中核苷、虫草酸及蛋白质营养价值都高于虫体。

关键词: 蝼蛄虫草, 核苷, 虫草酸, 氨基酸

Abstract: In order to detect nucleoside, cordycepic acid and amino acid contents in insect body and stroma of Cordyceps gryllotalpae, ultrasound technology was used in extracting water-soluble constituents of C. gryllotalpae, and HPLC detection system was established and used in detecting nucleoside, cordycepic acid and amino acid were detected respectively by spectrophotometer and automatic amino acid analyzer. The result showed that recovery of nucleoside detection was 95.73%-99.00%, the accuracy of appearance time was 0.05%-0.14%, the accuracy of peak area was 0.12%-0.84%, contents of uridine, inosine, vernine and adenosine of insect body and stroma were respectively 2.86, 0.43, 2.12, 0.17 and 3.53, 0.89, 3.51, 1.39 mg·g^-1. Recovery of cordycepic acid detection was 98.29%, cordycepic acid content in insect body and stroma were 109.64 and 127.46 mg·g^-1, and amino acid score of them were 56.3 and 62.8,essential amino acid index of them were 81.9 and 115.4. The result indicated that contents of nucleoside, cordycepic acid and nutritive value of protein in stroma were all more tnan those in insect body.

Key words: Cordyceps gryllotalpae, nucleoside, cordycepic acid, amino acid

中图分类号:

S567.35

叶日英, 王雅玲, 侯宏伟, 张春辉, 孙力军, 徐德锋. 蝼蛄虫草的虫体与草体中核苷、虫草酸及氨基酸含量比较[J]. 浙江农业学报, 2017, 29(5): 824-830.

YE Riying, WANG Yaling, HOU Hongwei, ZHANG Chunhui, SUN Lijun, XU Defeng. Comparison and analysis of nucleoside, cordycepic acid and amino acid content in insect body and stroma of Cordyceps gryllotalpae[J]. , 2017, 29(5): 824-830.

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蝼蛄虫草的虫体与草体中核苷、虫草酸及氨基酸含量比较

Comparison and analysis of nucleoside, cordycepic acid and amino acid content in insect body and stroma of Cordyceps gryllotalpae

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