鸭IL-2基因启动子的克隆、多态性与表达的关联分析

doi:10.3969/j.issn.1004-1524.2016.12.04

浙江农业学报 ›› 2016, Vol. 28 ›› Issue (12): 1985-1991.DOI: 10.3969/j.issn.1004-1524.2016.12.04

鸭IL-2基因启动子的克隆、多态性与表达的关联分析

张涛, 刘贺贺^*, 罗俊, 李亮, 黄惠兰, 何桦

四川农业大学畜禽遗传资源发掘与创新利用四川省重点实验室,四川成都 611130

收稿日期:2016-06-03 出版日期:2016-12-15 发布日期:2017-01-05
通讯作者: 刘贺贺,E-mail: liuee1985@sicau.edu.cn
作者简介:张涛(1991—),男,四川西昌人,硕士研究生,从事动物遗传育种与繁殖研究。E-mail: 448939460@qq.com
基金资助:
四川省科技支撑项目(2015JY0110)

Cloning of duck IL-2 gene promoter and correlation analysis about polymorphism in the promoter region with its expression levels

ZHANG Tao, LIU He-he^*, LUO Jun, LI Liang, HUANG Hui-lan, HE Hua

Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu 611130, China

Received:2016-06-03 Online:2016-12-15 Published:2017-01-05

摘要/Abstract

摘要： 为研究鸭IL-2基因调控区单核苷酸多态性对其转录调控的影响,克隆获得了鸭IL-2基因启动子2 850 bp序列,与人、小鼠和原鸡的同源性分别为35.37%,37.52%和34.74%。其中,-1 400/-1 000存在集中的核心转录因子结合位点。对鸭IL-2基因启动子(-1 932/-742)进行单核苷酸多态检测和遗传多态性分析发现,该区域存在两个突变位点(C-1353A、C-1406T),且均处于Hardy-Weinberg极不平衡状态;等位基因A均为优势等位基因。单核苷酸多态与其表达水平的相关性分析发现,突变位点不同基因型与IL-2基因mRNA表达水平和IL-2蛋白水平均无显著相关关系,但基因型AA个体的mRNA表达量均高于其他基因型个体(P>0.05),表明鸭IL-2启动子等位基因A可能有促进IL-2基因转录的趋势。研究结果为IL-2基因转录调控机制的研究提供了理论基础。

关键词: 鸭, IL-2, 单核苷酸多态, 转录调控区, 转录水平

Abstract: To study the effect of single nucleotide polymorphism (SNP) of duck IL-2 gene regulatory region on transcriptional regulation, a 2 850 bp promoter sequence of duck IL-2 gene was obtained by cloning. The homology analysis showed that the homology of duck IL-2 promoter sequence with human, mouse and chicken was 35.37%, 37.52% and 34.74%, respectively. The core transcription factor binding sites was concentrated at the region (-1400/-1000). The detecting of SNP and genetic polymorphism of duck IL-2 gene promoter fragment (-1932/-742) found two polymorphism sites (C-1406T and C-1353A). Allele A was the predominant allele, and both polymorphism sites were not in Hardy-Weinberg. Correlation analysis showed that the mRNA expression levels of IL-2 gene and IL-2 protein levels had no significant relationship with different genotypes of the polymorphism sites. However, the expression levels of IL-2 mRNA in individuals with genotype AA were higher than those in other genotypes (P>0.05), suggesting that the allele A might promote the transcription of IL-2 gene. The results provided a theoretical basis for studies on the transcription regulation mechanism of IL-2 gene.

Key words: duck, IL-2, single nucleotide polymorphism, transcriptional regulation region, transcription level

中图分类号:

S834
Q754

张涛, 刘贺贺, 罗俊, 李亮, 黄惠兰, 何桦. 鸭IL-2基因启动子的克隆、多态性与表达的关联分析[J]. 浙江农业学报, 2016, 28(12): 1985-1991.

ZHANG Tao, LIU He-he, LUO Jun, LI Liang, HUANG Hui-lan, HE Hua. Cloning of duck IL-2 gene promoter and correlation analysis about polymorphism in the promoter region with its expression levels[J]. , 2016, 28(12): 1985-1991.

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鸭IL-2基因启动子的克隆、多态性与表达的关联分析

Cloning of duck IL-2 gene promoter and correlation analysis about polymorphism in the promoter region with its expression levels

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