褪黑素受体Mel 1b基因mRNA和蛋白在鸭不同组织中的表达与分布

doi:10.3969/j.issn.1004-1524.2018.05.06

浙江农业学报 ›› 2018, Vol. 30 ›› Issue (5): 711-716.DOI: 10.3969/j.issn.1004-1524.2018.05.06

褪黑素受体Mel 1b基因mRNA和蛋白在鸭不同组织中的表达与分布

刘文举¹, 王淑娟^1,*, 刘晓丽², 庞训胜¹, 王立克¹

1.安徽科技学院动物科学学院,安徽凤阳 233100;
2. 华中农业大学动物医学院,湖北武汉 430070

收稿日期:2017-09-25 出版日期:2018-05-20 发布日期:2018-05-23
通讯作者: 王淑娟,E-mail: wangshujuan2012@hotmail.com
作者简介:刘文举(1981—),男,河南驻马店人,硕士,从事动物生殖生理研究。E-mail: liuwenju2014@hotmail.com
基金资助:
国家自然科学基金(31301972); 安徽省自然科学基金(1308085QC66); 安徽省优秀青年人才支持计划(gxyq2018049); 安徽省教育厅自然科学基金(kj20136077)

Expression and distribution of Mel 1b mRNA and protein in various tissues of duck

LIU Wenju¹, WANG Shujuan^1,*, LIU Xiaoli², PANG Xunsheng¹, WANG Like¹

1. College of Animal Science, Anhui Science and Technology University, Fengyang 233100, China;
2. College of Animal Science, Huazhong Agricultural University, Wuhan 430070, China

Received:2017-09-25 Online:2018-05-20 Published:2018-05-23

摘要/Abstract

摘要： 褪黑素广泛的生理作用是通过与其膜受体(Mel 1a、Mel 1b、Mel 1c)结合介导的。褪黑素受体在机体内广泛分布,但因受体亚型、物种、组织不同而异。采用RT-PCR、real-time PCR和免疫组化技术探究了Mel 1b在鸭不同组织(包括鸭脾脏、心脏、肾脏、大脑、胸肌、卵巢、肺脏、肝脏、胰脏组织)中的表达分布及相对表达量。结果表明,鸭Mel 1b mRNA在脾脏、心脏、肾脏、大脑、胸肌、卵巢、肺脏、肝脏、胰脏中均有表达,且Mel 1b受体蛋白均存在于大脑、肺脏、肝脏、胸肌、肾脏、心脏、胰脏中(脾脏、卵巢未成功测试)。Real-time PCR结果表明,各组织中Mel 1b mRNA表达量存在差异,在卵巢中的表达量最高,其次为肺脏,在脾脏、心脏、肾脏和肝脏中的表达量也明显高于大脑中的表达,但在胸肌和胰脏中的表达量要稍低于大脑中的表达量。Mel 1b在鸭各组织中普遍表达分布,进一步说明了其介导褪黑素广泛的生理功能,特别是对生殖功能的调节。

关键词: 褪黑素受体, Mel 1b, 鸭, real-time PCR

Abstract: A broad physiological function of melatonin is mediated by binding to melatonin receptors (Mel 1a, Mel 1b and Mel 1c). Melatonin receptors are widely distributed in the body, but vary according to subtypes, species and tissues. The study investigated the expression, distribution of Mel 1b in different tissues of ducks (including heart, spleen, brain, kidney, liver, lung, pancreas and breast muscle) using RT-PCR, real time PCR and immunohistochemistry. The results showed that Mel 1b mRNA was extensively expressed in heart, spleen, brain, kidney, liver, lung, pancreas and breast muscle of ducks, and Mel 1b protein was distributed in brain, lung, liver, skeletal muscle, kidney, heart and pancreas. The expression levels of Mel 1b mRNA were different among various tissues. The highest expression of Mel 1b mRNA was found in ovary, followed by the lung. Compared with the expression level of Mel 1b in brain, the expression levels in spleen, heart kidney and liver were higher, but those in breast muscle and pancreas were lower. Thus, Mel 1b was widely distributed in duck tissues, which further confirmed its involvement in the broad physiological functions of melatonin, especially the regulation of reproduction.

Key words: melatonin receptor, Mel 1b, duck, real-time PCR

中图分类号:

S834

刘文举, 王淑娟, 刘晓丽, 庞训胜, 王立克. 褪黑素受体Mel 1b基因mRNA和蛋白在鸭不同组织中的表达与分布[J]. 浙江农业学报, 2018, 30(5): 711-716.

LIU Wenju, WANG Shujuan, LIU Xiaoli, PANG Xunsheng, WANG Like. Expression and distribution of Mel 1b mRNA and protein in various tissues of duck[J]. , 2018, 30(5): 711-716.

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褪黑素受体Mel 1b基因mRNA和蛋白在鸭不同组织中的表达与分布

Expression and distribution of Mel 1b mRNA and protein in various tissues of duck

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