鹅褪黑素受体Mel1c基因的组织表达特征

doi:10.3969/j.issn.1004-1524.2018.09.02

浙江农业学报 ›› 2018, Vol. 30 ›› Issue (9): 1454-1459.DOI: 10.3969/j.issn.1004-1524.2018.09.02

鹅褪黑素受体Mel1c基因的组织表达特征

王淑娟, 刘文举^*, 闻爱友, 庞训胜

安徽科技学院动物科学学院,安徽凤阳 233100

收稿日期:2018-05-07 出版日期:2018-09-25 发布日期:2018-10-15
作者简介:王淑娟(1980—),女,山东菏泽人,博士,讲师,主要从事动物生殖生理研究。E-mail: wangshujuan2012@hotmail.com

Expression of Mel1c mRNA in various tissues of goose

WANG Shujuan, LIU Wenju^*, WEN Aiyou, PANG Xunsheng

College of Animal Science, Anhui Science and Technology University, Fengyang 233100, China

Received:2018-05-07 Online:2018-09-25 Published:2018-10-15
Contact: 刘文举,E-mail: liuwenju2014@hotmail.com
Supported by:
安徽省优秀青年人才支持计划(gxyq2018049); 国家自然科学基金(31301972); 安徽省自然科学基金(1308085QC66)

摘要/Abstract

摘要： 采用qRT-PCR等技术探究了Mel1c在鹅不同器官组织(心脏、肝脏、肾脏、肺脏、胰脏、脾脏、卵巢、大脑、胸肌及F1、F2、F3、F4、F5卵泡)中的表达分布。结果表明,Mel1c mRNA在鹅的心脏、肝脏、肾脏、肺脏、胰脏、脾脏、卵巢、大脑、胸肌及各级卵泡中均有表达;其表达量在各组织中存在差异,且以胰脏、脾脏、肾脏、卵巢中表达量较高,肺脏次之,心脏、胸肌、大脑水平相当,肝脏中最少。而在卵泡组织中,F4级卵泡中最高,其次为F5级卵泡,F1级卵泡中表达量最少。Mel1c在鹅各组织中普遍表达分布,进一步说明了褪黑素在机体中有着广泛的生物学作用,特别是对卵泡发育的影响。

关键词: Mel1c, 褪黑素受体, 鹅, 表达分布

Abstract: This research investigated the expression of Mel1c in different tissues of goose (including heart, liver, kidney, lung, pancreas, spleen, ovary, brain, breast muscle and F1, F2, F3, F4, F5 stage follicles) using qRT-PCR. The results showed that Mel1c mRNA was expressed in the above mentioned tissues of goose, but the expression levels of Mel1c mRNA were different among various tissues. The expression levels of Mel1c mRNA in pancreas, spleen, kidney and ovary were higher than that in other tissues, followed by lung, the expressions in heart, breast muscle and brain were at a similar level, and the least was in liver. The expression levels of Mel1c mRNA were also different among various stages of follicles. The highest expression of Mel1c mRNA was found in F4 follicles, followed by F5 follicles, and the least was in F1 follicles. Thus, Mel1c was widely expressed in goose tissues, which further confirmed its involvement in the broad physiological functions of melatonin, especially the development of follicles in ovary.

Key words: Mel1c, melatonin receptor, goose, expression

中图分类号:

S852.2

王淑娟, 刘文举, 闻爱友, 庞训胜. 鹅褪黑素受体Mel1c基因的组织表达特征[J]. 浙江农业学报, 2018, 30(9): 1454-1459.

WANG Shujuan, LIU Wenju, WEN Aiyou, PANG Xunsheng. Expression of Mel1c mRNA in various tissues of goose[J]. , 2018, 30(9): 1454-1459.

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鹅褪黑素受体Mel1c基因的组织表达特征

Expression of Mel1c mRNA in various tissues of goose

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