高山马铃薯种质资源遗传多样性的同工酶分析

doi:10.3969/j.issn.1004-1524.2018.09.01

浙江农业学报 ›› 2018, Vol. 30 ›› Issue (9): 1445-1453.DOI: 10.3969/j.issn.1004-1524.2018.09.01

高山马铃薯种质资源遗传多样性的同工酶分析

洪森荣, 张铭心, 叶思雨, 宁本松

上饶师范学院生命科学学院,江西上饶 334001

收稿日期:2017-11-27 出版日期:2018-09-25 发布日期:2018-10-15
作者简介:洪森荣(1974—),男,江西永新人,硕士,教授,主要从事植物生物技术方面的研究。E-mail: hongsenrong@163.com

Genetic diversity analysis of alpine potato germplasm resources by isozyme

HONG Senrong, ZHANG Mingxin, YE Siyu, NING Bensong

College of Life Sciences, Shangrao Normal University, Shangrao 334001, China

Received:2017-11-27 Online:2018-09-25 Published:2018-10-15
Supported by:
上饶师范学院2017年大学生创新创业训练计划(2017-CX-49)

摘要/Abstract

摘要： 对中国云南德宏和曲靖、湖北恩施、江西怀玉山高山马铃薯种质资源的过氧化物酶(POD)、酯酶(EST)和多酚氧化酶(PPO)同工酶进行了分析研究,以期揭示其遗传多样性。结果表明,云南德宏高山马铃薯、云南曲靖高山马铃薯和湖北恩施高山马铃薯均属于同一类,遗传分化程度较低,而怀玉山高山马铃薯单独成一类;用块茎和叶片做同工酶检测,会造成结果的差异,因而应保证取材的一致性。该实验结果可为进一步开展高山马铃薯品种遗传改良、杂交亲本选配以及高山马铃薯种质资源的利用提供依据。

关键词: 高山马铃薯, 种质资源, 遗传多样性, 同工酶分析

Abstract: The genetic diversity of alpine potato germplasm resources in Yunnan Dehong, Yunnan Qujing, Hubei Enshi and Jiangxi Huaiyushan was analyzed by peroxidase (POD), esterase (EST) and polyphenol oxidase (PPO) isozyme in this paper. The results showed that Yunnan Dehong alpine potato, Yunnan Qujing alpine potato and Hubei Enshi alpine potato belonged to the same type, and the degree of their genetic differentiation was low, while the alpine potato in Jiangxi Huaiyushan was a separate group. Isozyme analysis of tubers and leaves would result in the difference of results, and isozyme analysis should ensure the consistency of sampling. The results of this study can provide the basis for further genetic improvement of potato varieties, selection of hybrid parents and utilization of alpine potato germplasm resources.

Key words: alpine potato, germplasm resources, genetic diversity, isozyme analysis

中图分类号:

S532

洪森荣, 张铭心, 叶思雨, 宁本松. 高山马铃薯种质资源遗传多样性的同工酶分析[J]. 浙江农业学报, 2018, 30(9): 1445-1453.

HONG Senrong, ZHANG Mingxin, YE Siyu, NING Bensong. Genetic diversity analysis of alpine potato germplasm resources by isozyme[J]. , 2018, 30(9): 1445-1453.

参考文献

[1] 马永强,朱海霞,郭青云.马铃薯晚疫病病害动态规律及药剂防治技术研究[J].江苏农业科学,2017,45(20):123-125.
MA Y Q, ZHU H X, GUO Q Y. Study on potato late blight disease dynamics and chemical control techniques[J]. Jiangsu Agricultural Sciences , 2017, 45(20): 123-125. (in Chinese)
[2] 姚志,谢云.主粮化背景下高山马铃薯产业化困境研究:以恩施市为例[J].长江大学学报(自然科学版),2016,13(3):54-56.
YAO Z, XIE Y. Study on potato industrialization dilemma in high mountain area under the background of the staple food: taking Enshi city as an example[J]. Journal of Yangtze University ( Natural Science Edition ), 2016, 13(3): 54-56. (in Chinese)
[3] 陈立强,师尚礼,马春晖.野生及栽培苜蓿种质资源遗传多样性的同工酶分析[J].草业科学,2014,31(6):1070-1079.
CHEN L Q, SHI S L, MA C H. Genetic diversity analysis of wild and cultivated alfalfa germplasm resources by isozyme markers[J]. Pratacultural Science , 2014, 31(6): 1070-1079. (in Chinese with English abstract)
[4] 隋益虎,李敏,胡能兵,等.32份辣椒种质遗传多样性的同工酶分析[J].西北植物学报,2014,34(5):908-914.
SUI Y H, LI M, HU N B, et al. Genetic diversity among 32 germplasms of the genus capsicum by isozyme analysis[J]. Acta Botanica Boreali-Occidentalia Sinica , 2014, 34(5): 908-914. (in Chinese with English abstract)
[5] 刘贺贺,蒋红霞,富贵,等.青藏高原蕨麻种质资源遗传多样性POD同工酶分析[J].西北农业学报,2016,25(3):413-422.
LIU H H, JIANG H X, FU G, et al. Genetic diversity of Potentilla anserine in Qinghai-Tibetan plateau of China based on peroxidase isozyme[J]. Acta Agriculturae Boreali-occidentalis Sinica , 2016, 25(3): 413-422. (in Chinese with English abstract)
[6] 洪森荣,周宇瑶,黄慧.上饶早梨离体保存库再生苗遗传稳定性的同工酶分析[J].基因组学与应用生物学,2018,35(12):3552-3561.
HONG S R, ZHOU Y Y, HUANG H. Isoenzyme analysis of genetic stability of the plantlets regenerated from conservation bank in vitro of early pear in Shangrao[J]. Genomics and Applied Biology , 2018, 35(12): 3552-3561. (in Chinese with English abstract)
[7] 尹明华,张铭心,李远芳,等.红芽芋低温疗法脱毒苗遗传稳定性同工酶检测[J].分子植物育种,2018,16(8):2571-2576.
YIN M H, ZHANG M X, LI Y F, et al. Isoenzyme detection of genetic stability of red bud taro virus-free plantlets by cryotherapy[J]. Molecular Plant Breeding , 2018, 16(8):2571-2576. (in Chinese with English abstract)
[8] 张春晓,李悦,沈熙环.林木同工酶遗传多样性研究进展[J].北京林业大学学报,1998,20(3):58-66.
ZHANG C X, LI Y, SHEN X H. A review on forest trees genetic diversity at the isozyme level[J]. Journal of Beijing Forestry University , 1998, 20(3):58-66. (in Chinese with English abstract)
[9] 丁玲,陈发棣,滕年军,等.菊花品种间过氧化物酶、酯酶同工酶的遗传多样性分析[J].中国农业科学,2008,41(4):1142-1150.
DING L, CHEN F D, TENG N J, et al. Analysis of genetic diversity of cultivars in Dendranthema×grandiflorum based on POD and SOD isozyme[J]. Scientia Agricultura Sinica , 2008, 41(4): 1142-1150. (in Chinese with English abstract)
[10] 丁玲,陈发棣,房伟民.菊属8个种27份材料遗传多样性的同工酶分析[J].西北植物学报,2007,27(2):249-256.
DING L, CHEN F D, FANG W M. Genetic diversity among 27 materials in 8 species of Dendranthema by isozyme analysis[J]. Acta Botanica Boreali-Occidentalia Sinica , 2007, 27(2):249-256. (in Chinese with English abstract)
[11] 王述民,谭富娟,胡家蓬.小豆种质资源同工酶遗传多样性分析与评价[J].中国农业科学,2002,35(11):1311-1318.
WANG S M, TAN F J, HU J P. Genetic diversity assessment of Adzuki bean germplasm resources based on isozyme analysis[J]. Scientia Agricultura Sinica , 2002, 35(11):1311-1318. (in Chinese with English abstract)
[12] 胡树贵,李艳.44个百合品种同工酶遗传多样性研究[J].西部林业科学,2009,38(4):27-35.
HU S G, LI Y. Genetic diversity of 44 lily varieties from isozyme study[J]. Journal of West China Forestry Science , 2009, 38(4): 27-35. (in Chinese with English abstract)
[13] 陈万秋,叶新太,李思光,等.猕猴桃酯酶同工酶和过氧化物酶同工酶分析及遗传多样性研究[J].南昌大学学报(理科版),2001,25(3):269-272.
CHEN W Q, YE X T, LI S G, et al. Analysis of EST and POX isozyme and genetic diversity of Actinidia chinensis planch [J]. Journal of Nanchang University ( Natural Science ), 2001, 25(3): 269-272. (in Chinese with English abstract)
[14] 李景欣,云锦凤,鲁洪艳,等.野生冰草种质资源同工酶遗传多样性分析与评价[J].中国草地,2005,27(6):34-38.
LI J X, YUN J F, LU H Y, et al. Genetic diversity assessment of Agropyron cristatum germplasm resources based on isozyme analysis[J]. Grassland of China , 2005, 27(6): 34-38. (in Chinese with English abstract)
[15] 沈镝,朱德蔚,李锡香,等.用5种同工酶分析云南芋种质资源的遗传多样性[J].植物遗传资源学报,2004,5(3):239-246.
SHEN D, ZHU D W, LI X X, et al. Analysis of genetic diversity of germplasm resources in taro using five isozymes[J]. Journal of Plant Genetic Resources , 2004, 5(3):239-246.(in Chinese with English abstract)
[16] 段艳凤,刘杰,卞春松,等.中国 88 个马铃薯审定品种 SSR 指纹图谱构建与遗传多样性分析[J].作物学报,2009,35(8):1451-1457.
DUAN Y F, LIU J, BIAN C S, et al. Construction of fingerprinting and analysis of genetic diversity with SSR markers for eighty-eight approved potato cultivars ( Solanum tuberosum L.) in China[J]. Acta Agronomica Sinica , 2009, 35(8):1451-1457.(in Chinese with English abstract)
[17] 田大翠,朱宏波,郭建夫,等.马铃薯遗传多样性的 ISSR分析[J].中国马铃薯,2010,24(1):1-5.
TIAN D C, ZHU H B, GUO J F, et al. Genetic diversity analysis based on potato ISSR[J]. Chinese Potato Journal , 2010, 24(1): 1-5.(in Chinese with English abstract)
[18] 何凤发,杨志平,张正圣,等.马铃薯遗传资源多样性的SRAP分析[J].农业生物技术学报,2007,15(6):1001-1005.
HE F F, YANG Z P, ZHANG Z S, et al. Genetic diversity analysis of potato germplasm by SRAP makers[J]. Journal of Agricultural Biotechnology , 2007, 15(6):1001-1005.(in Chinese with English abstract)
[19] 邸宏,陈伊里,金黎平.RAPD和AFLP标记分析中国马铃薯主要品种的遗传多样性[J].作物学报,2006,32(6):899-904.
DI H, CHEN Y L, JIN L P. Genetic diversity analysis of Chinese main potato cultivars by RAPD and AFLP makers[J]. Acta Agronomica Sinica , 2006, 32(6):899-904.(in Chinese with English abstract)
[20] 王清,黄惠英,陈亚兰,等.转基因纯合四倍体马铃薯多酚氧化酶活性及同工酶谱的变异[J].作物学报,2005,31(9):1162-1166.
WANG Q, HUANG H Y, CHEN Y L, et al. Variation of polyphenol oxidase activity and isozyme in transgenic homozygous tetraploid potato ( Solanum tuberosum L.)[J]. Acta Agronomica Sinica , 2005, 31(9): 1162-1166.(in Chinese with English abstract)
[21] 司怀军,戴朝曦.马铃薯种间体细胞杂种植株的细胞学观察和过氧化物同工酶谱分析[J].中国马铃薯,1998,12(4):195-198.
SI H J, DAI C X. Cytological observation and peroxidase isoenzyme analysis of inter-specific somatic hybrid plants in potato[J]. Chinese Potato Journal , 1998, 12(4): 195-198.(in Chinese with English abstract)
[22] 周浩宏,高锡忠.陕南马铃薯脱毒种薯高山繁育初报[J].陕西农业科学,2012 (6):92,202.
ZHOU H H, GAO X Z. Preliminary report on high yielding breeding of virus-free potato seeds in southern Shaanxi[J]. Shaanxi Journal of Agricultural Sciences , 2012 (6): 92, 202.(in Chinese)

高山马铃薯种质资源遗传多样性的同工酶分析

Genetic diversity analysis of alpine potato germplasm resources by isozyme

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