大豆异黄酮对雄性大鼠脾脏IL-2、IL-4、TNF-α、INF-γ蛋白表达的影响

doi:10.3969/j.issn.1004-1524.2017.09.06

浙江农业学报 ›› 2017, Vol. 29 ›› Issue (9): 1458-1464.DOI: 10.3969/j.issn.1004-1524.2017.09.06

大豆异黄酮对雄性大鼠脾脏IL-2、IL-4、TNF-α、INF-γ蛋白表达的影响

李立科¹, 罗启慧^{1, 2}, 黄超¹, 陈晓林¹, 陈苹¹, 李一帆¹, 刘文涛^{1, 2}, 陈正礼^{1, 2, *}

1.四川农业大学动物医学院,动物疾病模型实验室,四川成都 611130;
2.四川农业大学预防兽医研究所,四川成都 611130

收稿日期:2017-02-23 出版日期:2017-09-20 发布日期:2017-09-27
通讯作者: 陈正礼,E-mail:chzhli75@163.com
作者简介:李立科(1990—),男,四川成都人,硕士研究生,主要从事实验动物疾病模型研究。E-mail:394580507@qq.com
基金资助:
国家科技支撑计划课题(2014BAI03B01); 国家重大科学仪器设备开发专项(2013YQ49085906)

Effects of soy isoflavones on expression of IL-2, IL-4, TNF-α and INF-γ in male rats' spleen

LI Like¹, LUO Qihui^{1, 2}, HUANG Chao¹, CHEN Xiaolin¹, CHEN Ping¹, LI Yifan¹, LIU Wentao^{1, 2}, CHEN Zhengli^{1, 2, *}

1. Laboratory of Animal Disease Model,College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China;
2. Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China

Received:2017-02-23 Online:2017-09-20 Published:2017-09-27

摘要/Abstract

摘要： 试验旨在探讨不同剂量大豆异黄酮对雄性大鼠脾脏免疫功能的影响及其机理。将40只6周龄雄性SD大鼠随机分为4组,每组10只。对照组和低、中、高剂量组分别灌胃添加不同水平的大豆异黄酮(0、50、250、500 mg·kg^-1),大鼠每周称量体质量,连续干预4周后处死大鼠并记录脾脏质量;利用免疫组织化学链霉亲和素-生物素复合物(SABC)染色法研究大鼠脾脏中白介素-2(IL-2)、白介素-4(IL-4)、肿瘤坏死因子α(TNF-α)及干扰素γ(INF-γ)蛋白的表达水平。结果显示,与对照组相比,各组间脾脏指数差异不显著;中、高剂量组脾小体体积显著减小,红髓区淋巴细胞数量显著减少;IL-2在低剂量组表达显著降低,但在中、高剂量组表达增加且在高剂量组达到显著水平;IL-4、TNF-α和INF-γ在大豆异黄酮干预后各剂量组表达水平均显著降低。综上,高剂量的大豆异黄酮能够提高IL-2蛋白的表达水平,各剂量组总体抑制了IL-4、TNF-α和INF-γ蛋白的表达水平,这表明大豆异黄酮对雄性健康大鼠存在潜在的免疫抑制,且呈现一定的剂量差异。

关键词: 大豆异黄酮, 脾脏, 免疫功能, 大鼠

Abstract: In the present study, effects of different dose of soy isoflavones (SIF) on splenic immune function in male rats were studied and mechanism was explained. Forty rats aged 6 weeks were randomly divided four groups fed with SIF 0 (control group),50 (low dose group),250 (medium dose group), 500 (high dose group) mg·kg^-1 body weight. Body weight were recorded every week, and at the end of experiment the rats' spleens were separated and weighed. The expression levels of IL-2, IL-4, TNF-α and INF-γ in rats spleen were detected by immunohistochemistry SABC method. The results showed that compared with control group, there were no significantly differences in spleen index among the groups. Splenic corpuscles in middle and high group were smaller than those in other groups with the lymphocytes number significantly decreased in red pulp. The expression of IL-2 in low dose group was significantly decreased, but rapidly increased in middle and high dose group with a dose-dependent. However, the expression levels of IL-4, TNF-α and INF-γ were all significantly reduced after treated by isoflavones. All above,even though high dose isoflavones can increase IL-2 level,the expressions of IL-4, TNF-α and INF-γ were inhibited with the dose of isoflavones increasing, which indicated SIF had potential immunosuppression to male rats and appeared dosage difference.

Key words: soy isoflavone, spleen, immune function, rats

中图分类号:

李立科, 罗启慧, 黄超, 陈晓林, 陈苹, 李一帆, 刘文涛, 陈正礼. 大豆异黄酮对雄性大鼠脾脏IL-2、IL-4、TNF-α、INF-γ蛋白表达的影响[J]. 浙江农业学报, 2017, 29(9): 1458-1464.

LI Like, LUO Qihui, HUANG Chao, CHEN Xiaolin, CHEN Ping, LI Yifan, LIU Wentao, CHEN Zhengli. Effects of soy isoflavones on expression of IL-2, IL-4, TNF-α and INF-γ in male rats' spleen[J]. , 2017, 29(9): 1458-1464.

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大豆异黄酮对雄性大鼠脾脏IL-2、IL-4、TNF-α、INF-γ蛋白表达的影响

Effects of soy isoflavones on expression of IL-2, IL-4, TNF-α and INF-γ in male rats' spleen

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