浙江省三个斑鳜野生群体线粒体DNA细胞色素b基因序列的遗传变异

doi:10.3969/j.issn.1004-1524.2017.10.06

浙江农业学报 ›› 2017, Vol. 29 ›› Issue (10): 1637-1641.DOI: 10.3969/j.issn.1004-1524.2017.10.06

浙江省三个斑鳜野生群体线粒体DNA细胞色素b基因序列的遗传变异

许晓军, 孟庆辉, 陈小明

浙江省水产技术推广总站,浙江杭州 310023

收稿日期:2017-03-15 出版日期:2017-10-20 发布日期:2017-12-05
作者简介:许晓军(1979-),男,河南安阳人,博士研究生,高级工程师,从事水生生物遗传育种方向研究。E-mail: 24587980@qq.com
基金资助:
2013年度浙江省农业标准化研究项目“浙江省斑鳜种质特性研究”

Genetic variation of mitochondrial DNA Cyt b among three Siniperca scherzeri populations in Zhejiang Province

XU Xiaojun, MENG Qinghui, CHEN Xiaoming

Zhejiang Fisheries Technical Extension Center,Hangzhou 310023,China

Received:2017-03-15 Online:2017-10-20 Published:2017-12-05

摘要/Abstract

摘要： 对钱塘江上游江山、乌溪江段和瓯江上游云和段3个斑鳜(Siniperca scherzeri Steindachner)群体线粒体DNA细胞色素b(Cyt b)基因片段进行了扩增和测序,比较并分析了其遗传多样性和群体遗传分化。结果显示,在斑鳜Cytb基因965 bp的部分序列中,A+T含量(52.11%)略高于G+C含量(47.89%),77个个体中发现了16个多态性位点,8种单倍型。江山群体、乌溪江群体和云和群体的单倍型多样性分别为0.570±0.078、0.668±0.067、0.667±0.032,核苷酸多样性分别为0.001 8±0.000 3、0.001 9±0.000 2、0.004 3±0.000 6。江山群体与乌溪江群体间的Fst为-0.005 16(P>0.05),表明同为钱塘江上游的两群体没有明显分化;但云和群体与江山群体、乌溪江群体间Fst分别为0.191 23(P<0.01)和0.178 10(P<0.01),表明瓯江上游与钱塘江上游斑鳜群体存在显著的分化。

关键词: 斑鳜, 细胞色素b基因, 遗传变异

Abstract: The mtDNA Cyt b gene of Siniperca scherzeri Steindachner from the upper reaches of Qiantang River (Jiangshan population and Wuxijiang population) and the upper reaches of Oujiang River (Yunhe population) were amplified and sequenced to assess the genetic diversity and genetic structure. The results showed that the average content of A+T (52.11%) was higher than that of G+C (47.89%) in the aligned 965 bp partial sequences. A total of 16 polymorphic sites were detected and 8 haplotypes were recovered out of 77 individuals. The haplotype diversity of Jiangshan, Wuxijiang and Yunhe populations were 0.570±0.078, 0.668±0.067 and 0.667±0.032,respectively, while the nucleotide diversity of the three populations were 0.001 8±0.000 3、0.001 9±0.000 2、0.004 3±0.000 6,respectively. The Fst value between Jiangshan population and Wuxijiang population was low (Fst=-0.005 16, P>0.05), which indicated that the two upper reaches of Qiantang River populations did not have significant genetic differentiation. While statistically significant genetic differentiations were observed between Yunhe population and Jiangshan population (Fst=0.191 23, P<0.01), and between Yunhe population and Wuxijiang population (Fst=0.178 10, P<0.01), which indicated significant genetic differentiations between the upper reaches of Qiantang River and the upper reaches of Oujiang River.

Key words: Siniperca scherzeri Steindachner, cytochrome b gene, genetic variation

中图分类号:

S937

许晓军, 孟庆辉, 陈小明. 浙江省三个斑鳜野生群体线粒体DNA细胞色素b基因序列的遗传变异[J]. 浙江农业学报, 2017, 29(10): 1637-1641.

XU Xiaojun, MENG Qinghui, CHEN Xiaoming. Genetic variation of mitochondrial DNA Cyt b among three Siniperca scherzeri populations in Zhejiang Province[J]. , 2017, 29(10): 1637-1641.

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浙江省三个斑鳜野生群体线粒体DNA细胞色素b基因序列的遗传变异

Genetic variation of mitochondrial DNA Cyt b among three Siniperca scherzeri populations in Zhejiang Province

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