17个枸杞品种的SCoT遗传多样性

doi:10.3969/j.issn.1004-1524.2018.10.08

浙江农业学报 ›› 2018, Vol. 30 ›› Issue (10): 1665-1670.DOI: 10.3969/j.issn.1004-1524.2018.10.08

17个枸杞品种的SCoT遗传多样性

马利奋¹, 尹跃², 赵建华², 王亚军², 戴国礼², 安巍^{2, *}

1.宁夏枸杞产业发展中心,宁夏银川 750001;
2.宁夏农林科学院国家枸杞工程技术研究中心,宁夏银川 750002

收稿日期:2017-11-21 出版日期:2018-10-25 发布日期:2018-11-02
通讯作者: 安巍,E-mail:angouqi@163.com
作者简介:马利奋(1985—),女,宁夏吴忠人,硕士,助理工程师,主要从事枸杞栽培与育种研究。E-mail:lifenma_828@163.com
基金资助:
宁夏农业特色优势产业新品种选育专项(2013NYYZ0101)

Analysis on genetic diversity of 17 goji(wolfberry)cultivars by SCoT markers

MA Lifen¹, YIN Yue², ZHAO Jianhua², DAI Guoli², AN Wei^{2, *}

1. Wolfberry Industry Development Centre of Ningxia, Yinchuan 750001, China;
2.National Wolfberry Engineering Research Center, Ningxia Academy of Agriculture and Forestry Sciences, Yinchuan 750002, China

Received:2017-11-21 Online:2018-10-25 Published:2018-11-02

摘要/Abstract

摘要： 采用目标起始密码子多态性(SCoT)分子标记对17个枸杞品种进行遗传多样性分析。从80条引物中筛选出19条重复性好、条带清晰的引物进行PCR扩增,共扩增到96个条带,其中多态性条带71条,多态性比率为73.96%。多态信息含量(PIC)变化范围为0.055(SCoT-29)~0.239(SCoT-66),平均为0.132。聚类分析和主坐标分析结果表明,供试的17个枸杞品种遗传相似系数为0.43~1.00,在遗传相似系数为0.87处可将所有品种分为3个类群。综上,利用SCoT分子标记可揭示枸杞品种资源的遗传多样性,为枸杞品种资源保护和利用提供科学依据。

关键词: 枸杞, 品种, SCoT标记, 遗传多样性

Abstract: Genetic diversity of 17 goji (wolfberry) cultivars was analyzed by start codon targeted polymorphism (SCoT) molecular marker. Nineteen SCoT primers generating clear and reproducible bands were obtained through screening a total of eighty primers. A total of 96 bands were amplified, of which 71 (73.96%) were polymorphic. The value of polymorphism information content (PIC) varied from 0.055 (SCoT-29) to 0.239 (SCoT-66), with average of 0.132. Cluster analysis and principal coordinated analysis indicated that the genetic similarity (GS) varied from 0.43 to 1.00. All cultivars could be divided into three groups at the genetic similarity of 0.87. So SCoT markers could be effectively used to evaluate the genetic diversity of goji (wolfberry) cultivars germplasms. These results would provide references for protection and rational utilization of wolfberry cultivars.

Key words: goji (wolfberry), cultivar, SCoT marker, genetic diversity

中图分类号:

S567.19

马利奋, 尹跃, 赵建华, 王亚军, 戴国礼, 安巍. 17个枸杞品种的SCoT遗传多样性[J]. 浙江农业学报, 2018, 30(10): 1665-1670.

MA Lifen, YIN Yue, ZHAO Jianhua, DAI Guoli, AN Wei. Analysis on genetic diversity of 17 goji(wolfberry)cultivars by SCoT markers[J]. , 2018, 30(10): 1665-1670.

参考文献

[1] FUKUDA T, YOKOYAMA J, OHASHI H.Phylogeny and biogeography of the genus Lycium(Solanaceae): inferences from chloroplast DNA sequences[J]. Molecular Phylogenetics and Evolution, 2001, 19(2): 246-258.
[2] 利河伯, 姜丽. 枸杞出口趋势分析[J]. 中国现代中药, 2014, 16(3): 244-246.
LI H B, JIANG L.Analysis of wolfberry export trend[J]. Modern Chinese Medicine, 2014, 16(3): 244-246.(in Chinese)
[3] 曹有龙. 中国枸杞种质资源[M]. 北京: 中国林业出版社, 2015.
[4] CHENG K, CHANG H, H H. RAPD analysis of Lycium bararum medicine in Taiwan market[J]. Botanical Bulletin of Academia Sinica, 2000, 41(1): 11-14.
[5] KWON S J, LEE G A, LEE S Y, et al.Isolation and characterization of 21 microsatellite loci in Lycium chinense and cross-amplification in Lycium barbarum[J]. Conservation Genetics, 2009, 10(5): 1557-1560.
[6] CHEN H K, ZHONG Y.Microsatellite markers for Lycium ruthenicum(Solananeae)[J]. Molecular Biology Reports, 2014, 41(9): 5545-5548.
[7] 安巍, 王亚军, 尹跃, 等. 枸杞种质资源的SRAP分析[J]. 浙江农业学报, 2013, 25(6): 1234-1237.
AN W, WANG Y J, YIN Y, et al.SRAP analysis of wolfberry germplasms[J].Acta Agriculturae Zhejingensis, 2013, 25(6): 1234-1237. (in Chinese with English abstract)
[8] 阿力同·其米克, 王青锋, 杨春锋, 等. 新疆产药用植物黑果枸杞遗传多样性的ISSR分析[J]. 植物科学学报, 2013, 31(5): 517-524.
ALITONG Q M K, WANG Q F, YANG C F, et al. ISSR analysis on genetic diversity of medically important Lycium ruthenicum Murr. in Xingjiang[J]. Plant Science Journal, 2013, 31(5): 517-524.(in Chinese with English abstract)
[9] COLLARD B C, MACKILL D J.Start codon targeted (SCoT) polymorphism: a simple, novel DNA marker technique for generating gene-targeted markers in plants[J]. Plant Molecular Biology Reporter, 2009, 27(1): 86-93.
[10] PRATIK S, MAYA K, SOURAV J, et al.Start codon targeted (SCoT) polymorphism reveals genetic diversity in wild and domesticated populations of ramie (Boehmeria nivea L. Gaudich.), a premium textile fiber producing species[J]. Meta Gene, 2015, 3(C): 62-70.
[11] QUE Y, PAN Y B, LU Y, et al.Genetic analysis of diversity within a Chinese local sugarcane germplasm based on start codon targeted polymorphism[J]. BioMed Research International, 2014 (5): 468375.
[12] JIANG L F, QI X, ZHANG X Q, et al.Analysis of diversity and relationships among orchardgrass (Dactylis glomerata L.) accessions using start codon-targeted markers[J]. Genetics and Molecular Research, 2014, 13(2): 4406-4418.
[13] HUANG L, HUANG X, YAN H, et al.Constructing DNA fingerprinting of Hemarthria cultivars using EST-SSR and SCoT markers[J]. Genetic Resources and Crop Evolution, 2014, 61(6): 1047-1055.
[14] 杨祥燕, 蔡元保, 黄秋伟, 等. 番木瓜主栽品种SCoT指纹图谱构建及遗传变异分析[J]. 西北植物学报, 2013, 33(9): 1756-1761.
YANG X Y, CAI Y B, HUANG Q W, et al.SCoT fingerprints and genetic variations of the papaya (Carica papaya L.) major cultivars[J]. Acta Botanica Boreali-Occidentalia Sinica, 2013, 33(9): 1756-1761. (in Chinese with English abstract)
[15] 查美琴, 赵玉玲, 李疆, 等. 新疆枸杞种质资源遗传多样性分析及DNA指纹图谱构建[J]. 植物遗传资源学报, 2016, 17(6): 1065-1071.
ZHA M Q, ZHAO Y L, LI J, et al.Analysis of genetic diversity and construction of DNA fingerprint of Xingjiang wolfberry germplasm resources[J]. Journal of Plant Genetic Resources, 2016, 17(6): 1065-1071. (in Chinese with English abstract)
[16] ABUZAYED M, EL-DABBA N, FRARY A, et al.GDdom: an online tool for calculation of dominant marker gene diversity[J]. Biochemical Genetics, 2017, 55(2): 155-157.
[17] ROHLF F J.NTSYS-pc: numerical taxonomy and multivariate analysis system[M]. New York: Applied Biostatistics Inc., 1992.
[18] 尹跃, 安巍, 赵建华, 等. 枸杞品种SSR荧光指纹图谱构建及遗传关系分析[J]. 西北林学院学报, 2017, 32(1): 137-141.
YIN Y, AN W, ZHAO J H, et al.Fingerprinting and genetic diversity analysis of wolfberry cultivars using fluorescence-labeled SSR markers[J]. Journal of Northwest Forestry University, 2017, 32(1): 137-141. (in Chinese with English abstract)

编辑推荐

Metrics

阅读次数

全文

1409

HTML			PDF

最新录用	在线预览	正式出版	最新录用	在线预览	正式出版
0	0	0	0	0	1409

来源	本网站	其他网站

次数	963	446
比例	68%	32%

摘要

761

最新录用	在线预览	正式出版

0	0	761

来源	本网站	其他网站

次数	759	2
比例	100%	0%

17个枸杞品种的SCoT遗传多样性

Analysis on genetic diversity of 17 goji(wolfberry)cultivars by SCoT markers

RichHTML

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

[1]	孙达, 龚恕, 崔宏春, 郭敏明, 郑旭霞. 不同品种茶树春秋季鲜叶超微绿茶粉适制性研究[J]. 浙江农业学报, 2021, 33(3): 437-446.
[2]	谢子玉, 王可尔, 赵雯靓, 文祖会, 程林润, 徐丽珊. 不同肉色甘薯的营养成分与生物活性[J]. 浙江农业学报, 2021, 33(2): 183-192.
[3]	陈丹, 汤翠凤, 董超, 甘树仙, 李俊, 阿新祥, 张斐斐, 杨雅云, 牛赛赛, 戴陆园. 云南软米地方品种籽粒淀粉品质特性研究[J]. 浙江农业学报, 2021, 33(2): 203-214.
[4]	洪霞, 赵永彬, 屈为栋, 陈银龙, 邱莉萍, 王娇阳. 基于表型性状与简单重复序列标记的浙江省芋种质资源遗传多样性比较[J]. 浙江农业学报, 2020, 32(9): 1544-1554.
[5]	万志前, 陈晨. 植物新品种名称保护的再思考[J]. 浙江农业学报, 2020, 32(6): 1103-1111.
[6]	严福林, 王波, 温迪, 徐文芬, 孙庆文, 魏升华. 苗药小花清风藤叶绿体基因psbA-trnH序列特征及遗传多样性分析[J]. 浙江农业学报, 2020, 32(5): 810-815.
[7]	张小彦, 何静, 侯彩霞, 张树衡. 枸杞根腐病菌拮抗菌株的筛选与鉴定[J]. 浙江农业学报, 2020, 32(5): 858-865.
[8]	郭勤卫, 张婷, 刘慧琴, 章心惠, 李朝森, 项小敏, 赵东风, 万红建. 应用ISSR分子标记评价我国丝瓜种质资源遗传多样性[J]. 浙江农业学报, 2020, 32(4): 616-623.
[9]	刘予煊, 程焕, 叶兴乾, 刘慧燕, 方海田. 不同菌株发酵枸杞汁中生物活性物质与香气组成物质含量变化[J]. 浙江农业学报, 2020, 32(3): 499-509.
[10]	胡倩文, 徐延浩, 王容, 张文英, 华为, 吕超. 大麦4个穗部性状的关联分析[J]. 浙江农业学报, 2020, 32(11): 1941-1953.
[11]	万志前, 张媛. 实质性派生品种制度的缘起、困境与因应[J]. 浙江农业学报, 2020, 32(11): 2067-2076.
[12]	谢文钢, 李晓松, 李伟, 唐茜. 四川地方中小叶茶树资源的表型遗传多样性[J]. 浙江农业学报, 2019, 31(9): 1405-1415.
[13]	尹潇潇, 李燕方, 古江, 廖艳, 谢跃, 杨光友, 古小彬. 基于线粒体ATP6基因全序列分析中国绵羊痒螨(兔亚种)的遗传多样性[J]. 浙江农业学报, 2019, 31(8): 1231-1238.
[14]	唐首杰, 毕详, 张飞明, 张友良. 连续三代减数分裂雌核发育团头鲂的遗传多样性分析和RAPD鉴别方法的建立[J]. 浙江农业学报, 2019, 31(8): 1257-1271.
[15]	岳高红, 潘彬荣, 刘永安, 梅喜雪, 许立奎, 张宗宸, 周志辉. 利用SSR分析浙南地区甜玉米自交系的遗传多样性[J]. 浙江农业学报, 2019, 31(7): 1029-1036.