植物杆菌属(Plantibacter)菌株WZW03的分子标记与定殖促生能力

doi:10.3969/j.issn.1004-1524.2018.10.15

浙江农业学报 ›› 2018, Vol. 30 ›› Issue (10): 1715-1721.DOI: 10.3969/j.issn.1004-1524.2018.10.15

植物杆菌属(Plantibacter)菌株WZW03的分子标记与定殖促生能力

王志刚^{1, 2}, 刘泽平¹, 胡云龙¹, 刘虹¹, 朱晓慧¹

1.齐齐哈尔大学生命科学与农林学院,黑龙江齐齐哈尔 161006;
2.农业农村部华南都市农业重点实验室,广东广州 510640

收稿日期:2018-01-17 出版日期:2018-10-25 发布日期:2018-11-02
作者简介:王志刚(1980—),男,内蒙古赤峰人,教授,博士,主要从事土壤微生物方面的研究。E-mail:wzg1980830@sina.com
基金资助:
黑龙江省普通本科高等学校青年创新人才培养计划(UNPYSCT-2015092)

Molecular marker and colonization of Plantibacter sp. WZW03

WANG Zhigang^{1, 2}, LIU Zeping¹, HU Yunlong¹, LIU Hong¹, ZHU Xiaohui¹

1. School of Life Science and Agriculture and Forestry, Qiqihar University, Qiqihar 161006, China;
2. Key Laboratory of Urban Agriculture in South China, Ministry of Agriculture and Rural Affairs, Guangzhou 510640, China

Received:2018-01-17 Online:2018-10-25 Published:2018-11-02

摘要/Abstract

摘要： 将荧光质粒POT2-RFP转入植物根际促生菌Plantibacter sp. WZW03进行荧光与氨苄青霉素(Amp)抗性标记,研究该菌株在西瓜根际的定殖促生能力。通过生物转化法成功获得遗传稳定的转化子Plantibacter sp. WZW03-P,Plantibacter sp. WZW03-P的生长特性与WZW03无显著差异。在菌浓度相同时,WZW03-P在西瓜幼根表面吸附50 min时吸附量最大,达到(50.87±0.06)×10⁵ cfu·g^-1;当吸附时间相同时,吸附量随着菌株浓度的增大而增加,吸附饱和浓度为(1.27±0.34)×10⁷ cfu·g^-1。在灭菌土和正常土中,WZW03-P数量随投放时间增加而下降,在20 d时活菌数稳定于10⁶ cfu·g^-1水平。WZW03-P对西瓜植株和根系生长具有显著促生效应,且菌株在西瓜根际定殖量较高,具有显著的根际定殖竞争优势。综上,Plantibacter sp. WZW03-P能够较好地吸附且定殖于西瓜根际,并具有促进西瓜生长的功能。

关键词: 分子标记, 根际促生菌, 西瓜

Abstract: In this study, the fluorescent plasmid POT2-RFP was transferred into Plantibacter sp. WZW03 as markers and the colonization of strain WZW03 was studied in watermelon rhizosphere. Transgenics with genetically stable transformants were successfully obtained in Plantibacter sp. WZW03-P, and growth characteristic of Plantibacter sp. WZW03-P was not significantly different from that of WZW03. Under the same bacterial concentration, WZW03-P had the largest adsorption capacity of (50.87±0.06)×10⁵ cfu·g^-1 when the watermelon root surface was adsorbed for 50 min. Under the same adsorption time, the adsorption capacity increased with the increase of the strain concentration and the saturation concentration of adsorption was (1.27±0.34)×10⁷ cfu·g^-1. The number of WZW03-P in sterilized soil and normal soil decreased with the prolonged feeding time, and the number of viable bacteria was stable at 10⁶ cfu·g^-1 at 20 d. The promoting effects of WZW03-P were found on the growth of watermelon plants and roots, and the colonization rate of WZW03-P in the watermelon rhizosphere was high, which showed that WZW03-P was provided with a significant competitive advantage in rhizosphere. In conclusion, Plantibacter sp. WZW03-P could colonize well in the watermelon rhizosphere, and promote the growth of watermelon.

Key words: molecular marker, plant growth-promoting rhizobacteria, watermelon

中图分类号:

S154.39

王志刚, 刘泽平, 胡云龙, 刘虹, 朱晓慧. 植物杆菌属(Plantibacter)菌株WZW03的分子标记与定殖促生能力[J]. 浙江农业学报, 2018, 30(10): 1715-1721.

WANG Zhigang, LIU Zeping, HU Yunlong, LIU Hong, ZHU Xiaohui. Molecular marker and colonization of Plantibacter sp. WZW03[J]. , 2018, 30(10): 1715-1721.

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植物杆菌属(Plantibacter)菌株WZW03的分子标记与定殖促生能力

Molecular marker and colonization of Plantibacter sp. WZW03

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