西兰花中3种杀菌剂的残留动态与风险评估

doi:10.3969/j.issn.1004-1524.2021.07.15

浙江农业学报 ›› 2021, Vol. 33 ›› Issue (7): 1292-1299.DOI: 10.3969/j.issn.1004-1524.2021.07.15

西兰花中3种杀菌剂的残留动态与风险评估

孙彩霞¹(), 欧阳志周²^,³, 刘玉红¹, 于国光¹

1.浙江省农业科学院农产品质量安全与营养研究所/省部共建农产品质量安全危害因子与风险防控国家重点实验室,浙江杭州 310021
2.天津大学环境科学与工程学院,天津 300072
3.齐鲁工业大学(山东省科学院) 环境科学与工程学院,山东济南 250000

收稿日期:2020-07-26 出版日期:2021-07-25 发布日期:2021-08-06
作者简介:孙彩霞(1981—),女,山东泰安人,博士研究生,副研究员,从事农产品质量安全研究。E-mail: suncaixia0571@126.com
基金资助:
国家重点研发计划(2018YFF0213505);国家重点研发计划(2018YFF0213406)

Residue dynamic and risk assessment of three fungicides in broccoli

SUN Caixia¹(), OUYANG Zhizhou²^,³, LIU Yuhong¹, YU Guoguang¹

1. State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products(SKLQSAP), Institute of Agro-Product Safety and Nutrition, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China
2. School of Environment Science and Engineering, Tianjin University, Tianjin 300072, China
3. Environmental Science and Engineering School, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250000, China

Received:2020-07-26 Online:2021-07-25 Published:2021-08-06

摘要/Abstract

摘要：

农药残留是影响我国蔬菜质量安全的重要因素,文章以西兰花为研究对象,开展了苯醚甲环唑、戊唑醇、腈菌唑等3种三唑类农药在西兰花中的残留动态试验。建立了GC-MS测定西兰花中3种三唑类农药残留的方法,并开展了在西兰花中残留的膳食暴露风险评估。结果表明,根据现有的膳食摄入数据计算,西兰花生产中使用苯醚甲环唑和腈菌唑应控制使用量,并建议设置28 d以上的安全间隔期;建议对西兰花生产使用戊唑醇的风险进行进一步评估。

关键词: 西兰花, 农药残留, 三唑类杀菌剂, 风险评估, 膳食摄入, 安全间隔期

Abstract:

Pesticide residue is the key impact factor that affect vegetable quality and safety. In this article, broccoli was studied and the residue dynamic trial of three triazole pesticides including difenoconazole, tebuconazole and myclobutanil was carried out. The GC-MS method was established to detect triazole pesticides residue and dietary intake riskassessment of the three pesticides was carried out. The results showed that, according to the dietary intake data, the usage of difenoconazole and myclobutanil should be controlled and post-harvest interval should be longer than 28 days, application of tebuconazole in broccoli should be assessed further.

Key words: broccoli, pesticide residue, triazole fungicide, risk assessment, dietary intake, preharvest-interval

中图分类号:

S481.8

孙彩霞, 欧阳志周, 刘玉红, 于国光. 西兰花中3种杀菌剂的残留动态与风险评估[J]. 浙江农业学报, 2021, 33(7): 1292-1299.

SUN Caixia, OUYANG Zhizhou, LIU Yuhong, YU Guoguang. Residue dynamic and risk assessment of three fungicides in broccoli[J]. Acta Agriculturae Zhejiangensis, 2021, 33(7): 1292-1299.

图/表 5

参考文献 15

[1]	顾宏辉, 王建升, 虞慧芳, 等. 浙江省西兰花产业未来发展的思考[J]. 浙江农业科学, 2021, 62(5):851-853.
	GU H H, WANG J S, YU H F, et al. Review of broccoli indurstry development in Zhejiang[J]. Journal of Zhejiang Aricultural Sciences, 2021, 62(5):851-853. (in Chinese)
[2]	孙彩霞, 张棋, 徐明飞, 等. 农药及产地环境对西兰花质量安全的影响与评价[J]. 浙江农业科学, 2015, 56(11):1708-1711.
	SUN C X, ZHANG Q, XU M F, et al. Effect and evaluation of pesticide and producing environment on broccoli quality and safety[J]. Journal of Zhejiang Agricultural Sciences, 2015, 56(11):1708-1711.(in Chinese)
[3]	徐明飞, 孙彩霞, 章虎, 等. 溴虫腈在露地和温室栽培西兰花花球及叶片的消解动态[J]. 浙江农业学报, 2015, 27(8):1435-1439.
	XU M F, SUN C X, ZHANG H, et al. Dissipation dynamics of chlorfenapyr in broccoli flowers and leaves under greenhouse and open field conditions[J]. Acta Agriculturae Zhejiangensis, 2015, 27(8):1435-1439.(in Chinese with English abstract)
[4]	周松, 张玉涛, 张秋云, 等. 三唑酮和苯醚甲环唑在草莓及土壤中残留消解动态[J]. 江苏农业科学, 2017, 45(15):164-166.
	ZHOU S, ZHANG Y T, ZHANG Q Y, et al. Degradation dynamics of triadimefon and difenoconazole residues in strawberry and soil[J]. Jiangsu Agricultural Sciences, 2017, 45(15):164-166.(in Chinese)
[5]	李菊颖, 严伟强, 何健, 等. 戊唑醇在环境中的降解迁移和生物富集性研究[J]. 生态毒理学报, 2017, 12(4):310-318.
	LI J Y, YAN W Q, HE J, et al. Degradation, migration and bioaccumulation of tebuconazole in the environment[J]. Asian Journal of Ecotoxicology, 2017, 12(4):310-318.(in Chinese with English abstract)
[6]	LIU Y P, SUN H B, LIU F M, et al. Dissipation and residue of myclobutanil in lychee[J]. Bulletin of Environmental Contamination and Toxicology, 2012, 88(6):902-905. DOI URL
[7]	WANG Z W, CANG T, QI P P, et al. Dissipation of four fungicides on greenhouse strawberries and an assessment of their risks[J]. Food Control, 2015, 55:215-220. DOI URL
[8]	MALHAT F, BADAWY H M A, BARAKAT D A, et al. Residues, dissipation and safety evaluation of chromafenozide in strawberry under open field conditions[J]. Food Chemistry, 2014, 152:18-22. DOI URL
[9]	安晶晶, 刘新刚, 董丰收, 等. 苯醚甲环唑在番茄和土壤中的残留动态研究[J]. 环境科学研究, 2009, 22(7):868-872.
	AN J J, LIU X G, DONG F S, et al. Dynamic analysis of difenoconazole residue in tomatoes and soil[J]. Research of Environmental Sciences, 2009, 22(7):868-872.(in Chinese with English abstract)
[10]	初春, 王志华, 秦冬梅, 等. 苯醚甲环唑在芹菜及其土壤中的残留测定和消解动态研究[J]. 中国科学: 化学, 2011, 41(1):129-135.
	CHU C, WANG Z H, QIN D M, et al. Study on determination and dynamics of difenoconazole residues in celery and soil[J]. Scientia Sinica (Chimica), 2011, 41(1):129-135.(in Chinese with English abstract)
[11]	孙明娜, 段劲生, 王梅, 等. 戊唑醇在葡萄和土壤中的残留和消解动态[J]. 农药学学报, 2013, 15(1):73-78.
	SUN M N, DUAN J S, WANG M, et al. Study on residues and dissipation of tebuconazole in grapes and soil[J]. Chinese Journal of Pesticide Science, 2013, 15(1):73-78.(in Chinese with English abstract)
[12]	陈莉, 戴荣彩, 陈家梅, 等. 戊唑醇在苹果和土壤中的残留动态[J]. 农药, 2007, 46(3):194-196.
	CHEN L, DAI R C, CHEN J M, et al. Residue dynamics of tebuconazole in apple and soil[J]. Agrochemicals, 2007, 46(3):194-196.(in Chinese with English abstract)
[13]	张志恒, 李红叶, 吴珉, 等. 百菌清、腈菌唑和吡唑醚菌酯在草莓中的残留及其风险评估[J]. 农药学学报, 2009, 11(4):449-455.
	ZHANG Z H, LI H Y, WU M, et al. Residue and risk assessment of chlorothalonil, myclobutanil and pyraclostrobin in greenhouse strawberry[J]. Chinese Journal of Pesticide Science, 2009, 11(4):449-455.(in Chinese with English abstract)
[14]	楼正云, 汤富彬, 罗逢健, 等. 腈菌唑在黄瓜和土壤中的残留动态[J]. 农药, 2008, 47(3):205-207.
	LOU Z Y, TANG F B, LUO F J, et al. Residue dynamics of myclobutanil in cucumber and soil[J]. Agrochemicals, 2008, 47(3):205-207.(in Chinese with English abstract)
[15]	张志恒, 袁玉伟, 郑蔚然, 等. 三唑磷残留的膳食摄入与风险评估[J]. 农药学学报, 2011, 13(5):485-495.
	ZHANG Z H, YUAN Y W, ZHENG W R, et al. Dietary intake and its risk assessment of triazophos residue[J]. Chinese Journal of Pesticide Science, 2011, 13(5):485-495.(in Chinese with English abstract)

农药 Pesticide	施用量Application amount		有效成分用量Active ingredient dosage
农药 Pesticide	1.0倍用药 1.0 time the pesticide	1.5倍用药 1.5 times the pesticide	1.0倍用药 1.0 time the pesticide	1.5倍用药 1.5 times the pesticide
苯醚甲环唑(10%水分散剂)	40.00	60.00	4.00	6.00
Difenoconazole (10% water dispersant)
戊唑醇(30%乳油) Tebuconazole (30% EC)	15.00	22.50	4.50	6.75
腈菌唑(25%乳油) Myclobutanil (25% EC)	40.00	60.00	10.00	15.00

农药 Pesticide	施用量Application amount		有效成分用量Active ingredient dosage
农药 Pesticide	1.0倍用药 1.0 time the pesticide	1.5倍用药 1.5 times the pesticide	1.0倍用药 1.0 time the pesticide	1.5倍用药 1.5 times the pesticide
苯醚甲环唑(10%水分散剂)	40.00	60.00	4.00	6.00
Difenoconazole (10% water dispersant)
戊唑醇(30%乳油) Tebuconazole (30% EC)	15.00	22.50	4.50	6.75
腈菌唑(25%乳油) Myclobutanil (25% EC)	40.00	60.00	10.00	15.00

农药 Pesticide	线性范围 Linear range/ (μg·L^-1)	线性方程 Linear equation	相关系数 Correlation coefficient	检出限 Detection limit/ (μg·L^-1)	定量限 Limit of quantitation/ (μg·L^-1)	RSD/% (n=7)
苯醚甲环唑Difenoconazole	5~100	y=427.52x-29.9	0.994 0	1.04	3.46	13.2
戊唑醇Tebuconazole	1~100	y=309.12x+27.5	0.995 4	0.24	0.79	11.4
腈菌唑Myclobutanil	1~100	y=643.24x-45.1	0.997 6	0.14	0.46	10.3

农药 Pesticide	线性范围 Linear range/ (μg·L^-1)	线性方程 Linear equation	相关系数 Correlation coefficient	检出限 Detection limit/ (μg·L^-1)	定量限 Limit of quantitation/ (μg·L^-1)	RSD/% (n=7)
苯醚甲环唑Difenoconazole	5~100	y=427.52x-29.9	0.994 0	1.04	3.46	13.2
戊唑醇Tebuconazole	1~100	y=309.12x+27.5	0.995 4	0.24	0.79	11.4
腈菌唑Myclobutanil	1~100	y=643.24x-45.1	0.997 6	0.14	0.46	10.3

农药 Pesticide	加标浓度 Spiked concentration/(μg·L^-1)	西兰花样品Broccoli sample(n=3)
农药 Pesticide	加标浓度 Spiked concentration/(μg·L^-1)	回收率Recovery rate/%	相对标准偏差RSD/%
苯醚甲环唑Difenoconazole	20	88.4	12.9
	50	117.8	2.8
	100	115.1	15.6
戊唑醇Tebuconazole	20	82.8	8.7
	50	109.8	14.9
	100	116.4	10.3
腈菌唑Myclobutanil	20	103.4	13.1
	50	107.6	10.3
	100	111.8	16.1

西兰花中3种杀菌剂的残留动态与风险评估

Residue dynamic and risk assessment of three fungicides in broccoli

RichHTML

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

图/表 5

参考文献 15

相关文章 15

编辑推荐

Metrics

本文评价

剂量 Dose	用药次数 Number of medications	栽培模式 Cultivation mode		苯醚甲环唑 Difenoconazole	戊唑醇 Tebuconazole	腈菌唑 Myclobutanil
1.0倍剂量 1.0 time the dose	1次用药 1 dose	露地 Open field	降解方程 Degradation equation	y=0.855+0.154x	y=0.872+0.172x	y=0.877+0.194x
			r	0.968	0.914	0.964
			T_1/2/d	4.50	4.03	3.29
		大棚 Greenhouse	降解方程 Degradation equation	y=0.810+0.150x	y=0.805+0.166x	y=0.839+0.16x
			r	0.973	0.951	0.934
			T_1/2/d	4.62	4.17	4.33
	2次用药 2 dose	露地 Open field	降解方程 Degradation equation	y=0.875+0.127x	y=0.836+0.094x	y=0.738+0.112x
			r	0.962	0.984	0.906
			T_1/2/d	5.45	7.37	6.18
		大棚 Greenhouse	降解方程 Degradation equation	y=0.766+0.122x	y=0.852+0.067x	y=0.821+0.101x
			r	0.947	0.978	0.969
			T_1/2/d	5.68	10.34	6.86
1.5倍剂量 1.5 times the dose	1次用药 1 dose	露地 Open field	降解方程 Degradation equation	y=0.522+0.136x	y=0.476+0.226x	y=0.539+0.166x
			r	0.934	0.925	0.942
			T_1/2/d	5.09	3.06	4.17
		大棚 Greenhouse	降解方程 Degradation equation	y=0.529+0.125x	y=0.519+0.196x	y=0.533+0.133x
			r	0.958	0.913	0.961
			T_1/2/d	4.58	3.53	5.21
	2次用药 2 dose	露地 Open field	降解方程 Degradation equation	y=0.571+0.11x	y=0.632+0.063x	y=0.558+0.078x
			r	0.901	0.924	0.949
			T_1/2/d	6.30	11	8.88
		大棚 Greenhouse	降解方程 Degradation equation	y=0.556+0.105x	y=0.566+0.059x	y=0.616+0.069x
			r	0.922	0.947	0.933
			T_1/2/d	6.60	11.74	10.04

剂量 Dose	安全间隔期 PHI/d	栽培模式 Cultivation mode	苯醚甲环唑Difenoconazole			戊唑醇Tebuconazole			腈菌唑Myclobutanil
剂量 Dose	安全间隔期 PHI/d	栽培模式 Cultivation mode	CRL/ (mg·kg^-1)	NEDI	RQ	CRL/ (mg·kg^-1)	NEDI	RQ	CRL/ (mg·kg^-1)	NEDI	RQ
1.0倍剂量	14	露地Open field	0.104	0.047 3	4.73	0.106	0.0482	1.61	0.142	0.064 6	2.15
1.0 times		大棚Greenhouse	0.114	0.051 8	5.18	0.132	0.0601	2.01	0.124	0.056 4	1.88
the dose	21	露地Open field	0.046	0.020 9	2.09	0.076	0.0345	1.15	0.085	0.038 6	1.28
		大棚Greenhouse	0.054	0.024 5	2.45	0.108	0.0491	1.638	0.069	0.031 4	1.04
	28	露地Open field	0.010	0.004 6	0.455	0.0280	0.0127	0.425	0.012	0.005 5	0.182
		大棚Greenhouse	0.011	0.005 0	0.500	0.071	0.0323	1.076	0.022	0.0100	0.333
1.5倍剂量	14	露地Open field	0.142	0.064 6	6.46	0.202	0.0919	3.06	0.186	0.0846	2.82
1.5 times		大棚Greenhouse	0.165	0.075 1	7.51	0.226	0.102 0	3.42	0.196	0.0891	2.97
the dose	21	露地Open field	0.106	0.048 2	4.82	0.176	0.080 0	2.67	0.136	0.0618	2.06
		大棚Greenhouse	0.102	0.046 4	4.64	0.208	0.0946	3.15	0.126	0.0573	1.91
	28 d	露地Open field	0.018	0.004 9	0.491	0.088	0.0400	1.33	0.062	0.0282	0.940
		大棚Greenhouse	0.022	0.0100	1.01	0.101	0.0459	1.53	0.086	0.0391	1.304
ADI(mg·kg^-1 bw)			0.01			0.03			0.03

[1]	王娣, 狄珊珊, 王新全, 张昌朋, 王祥云, 王萌, 章程辉. 豇豆不同生长时期施用毒死蜱的膳食风险[J]. 浙江农业学报, 2021, 33(6): 1104-1109.
[2]	田培, 赵慧宇, 刘之炜, 王娇, 狄珊珊, 徐浩, 汪志威, 王新全, 齐沛沛. 杨梅中灭蝇胺及其代谢物检测方法与风险评估[J]. 浙江农业学报, 2021, 33(3): 534-540.
[3]	张辉, 车旭升, 吕剑, 缑兆辉, 秦启杰, 罗建, 张国斌. 灌水量与氮素形态对西兰花生产和氮代谢的影响[J]. 浙江农业学报, 2021, 33(2): 308-315.
[4]	王娣, 狄珊珊, 王新全, 张昌朋, 王祥云, 王萌. 丁硫克百威在豇豆不同时期施用的降解代谢研究[J]. 浙江农业学报, 2020, 32(11): 2050-2058.
[5]	章丽君, 章虎, 徐明飞, 林春绵, 吴慧珍, 徐杰, 钱鸣蓉. 葡萄酿制过程中5种杀菌剂的残留变化[J]. 浙江农业学报, 2019, 31(1): 149-154.
[6]	方琦, 张俊, 周锦云. 加工过程对柑橘罐头多菌灵残留的影响[J]. 浙江农业学报, 2018, 30(9): 1599-1603.
[7]	虞游毅, 杨璐, 廖享, 程平, 武胜利, 李宏. 高效氯氟氰菊酯和氯氰菊酯在苹果中的残留降解动态及其去除方法[J]. 浙江农业学报, 2018, 30(8): 1376-1381.
[8]	葛婧, 蒋金花, 蔡磊明. 3种三唑类杀菌剂对斑马鱼的毒性研究[J]. 浙江农业学报, 2018, 30(5): 744-755.
[9]	李真, 孙彩霞, 戴芬, 郑蔚然, 于国光, 姚佳蓉, 王强. 食品安全国家标准GB 2763—2016对葡萄的新要求[J]. 浙江农业学报, 2018, 30(5): 840-847.
[10]	刘熔熔, 吴利红, 王增武. 浙江省油菜花期降水量风险评估[J]. 浙江农业学报, 2018, 30(10): 1624-1629.
[11]	刘新迎, 秦浩然, 袁玉伟, 姜风涛, 于国光, 娄德龙, 王士强, 张艳, 尹旭升. 山东省蜂蜜中铅含量调查与风险评估[J]. 浙江农业学报, 2017, 29(9): 1570-1574.
[12]	杨子峰, 陈伟强, 王伟, 李婉怡, 何贤彪, 杨祥田. 轮作模式对西兰花耕作土壤微生物和酶活性的影响[J]. 浙江农业学报, 2017, 29(5): 818-823.
[13]	章豪, 张宜文, 凌淑萍, 张亮, 陈若霞, 吴银良, 朱勇. 抑霉唑对葡萄的保鲜效果及其风险评估[J]. 浙江农业学报, 2017, 29(5): 840-844.
[14]	刘君, 朱良其, 黑银秀, 刘灿, 谢菲, 何道根. 西兰花苗期生长模型的拟合和分析[J]. 浙江农业学报, 2016, 28(12): 2040-2046.
[15]	徐丽红1，吴应淼2，蔡铮1，郑蔚然1，叶长文2，吴银华2. 不同栽培方式下灰树花有害重金属、农药残留及硒含量比较[J]. 浙江农业学报, 2016, 28(1): 79-.