浙江农业学报 ›› 2023, Vol. 35 ›› Issue (1): 112-120.DOI: 10.3969/j.issn.1004-1524.2023.01.12

• 植物保护 • 上一篇    下一篇

珍稀植物浙江七子花炭疽病病原菌的分离和鉴定

朱晏1(), 周梦亚1, 朱怡诺1, 何海叶1, 鲍洪华2, 张慧娟1, 蒋明1,*()   

  1. 1.台州学院 生命科学学院,浙江 台州 318000
    2.台州市生态环境保护局路桥分局,浙江 台州 318050
  • 收稿日期:2022-04-06 出版日期:2023-01-25 发布日期:2023-02-21
  • 通讯作者: *蒋明,E-mail:jiangming1973@139.com
  • 作者简介:朱晏(1999—),女,浙江长兴人,学士,研究方向为分子生物学。E-mail:1119190648@qq.com
  • 基金资助:
    国家级大学生创新创业训练计划(202110350016)

Isolation and identification of pathogen causing anthracnose disease on a rare plant species Heptacodium miconioides subsp. jasminoides

ZHU Yan1(), ZHOU Mengya1, ZHU Yinuo1, HE Haiye1, BAO Honghua2, ZHANG Huijuan1, JIANG Ming1,*()   

  1. 1. College of Life Sciences, Taizhou University, Taizhou 318000, Zhejiang, China
    2. Taizhou Municipal Ecology and Environment Bureau Luqiao Branch, Taizhou 318050, Zhejiang, China
  • Received:2022-04-06 Online:2023-01-25 Published:2023-02-21

摘要:

炭疽病是浙江七子花(Heptacodium miconioides subsp. jasminoides)的重要病害之一,该病的发生严重影响植株生长,并威胁该珍稀物种的生存。为明确引起浙江七子花炭疽病病原菌的种类,以病叶为材料,采用组织分离法获得病原真菌,在确定致病性的基础上,利用形态学和多基因联合法对炭疽病病原菌进行鉴定。结果表明,从叶片中共分离到3个菌株,经柯赫氏法则验证,证实仅1个菌株(QZH)能引起炭疽病;病原菌的形态特征与果生刺盘孢菌(Colletotrichum fructicola)一致。多基因联合分析结果显示,菌株QZH与果生刺盘孢菌在系统发育树上聚为一支,并具有较高的支持率。综合形态学特征与多基因鉴定鉴定结果,确定引起浙江七子花炭疽病的病原菌为果生刺盘孢菌。浙江七子花炭疽病病原菌的分离与鉴定为后续开展该病害的有效防治和致病机理研究提供了基础。

关键词: 浙江七子花, 炭疽病, 果生刺盘孢菌

Abstract:

Anthracnose is one of the most important diseases of Heptacodium miconioides subsp. jasminoides, which seriously affects plant growth and threatens the survival of this rare species. To clarify the causative pathogen, infected leaves of H. miconioides subsp. jasminoides were collected, and the pathogen was obtained by the tissue isolation method. After determining the pathogenicity of the isolated strains, both morphological and multi-gene approaches were applied to identify the causative pathogen. Three strains were isolated, and only one isolate namely QZH could cause anthracnose disease according to Koch’s Rule. The morphological characteristics were identical with those of Colletotrichum fructicola. The multi-gene analysis results indicated that QZH and C. fructicola shared the same clade, with high support rates. Based on the morphological characteristics and multi-gene identification results, it was confirmed that the pathogen causing anthracnose disease of H. miconioidessubsp. jasminoides was C. fructicola. These data provided a start point for both disease prevention and pathogenic mechanism studies in the future.

Key words: Heptacodium miconioides subsp. jasminoides, anthraconse disease, Colletotrichum fructicola

中图分类号: