浙江农业学报 ›› 2025, Vol. 37 ›› Issue (4): 790-799.DOI: 10.3969/j.issn.1004-1524.20240792

• 动物科学 • 上一篇    下一篇

浙江省地方品种鸡禽白血病的流行病学调查与净化策略评估

倪征1(), 卢磊2, 朱寅初1, 陈柳1, 李鑫基3, 叶伟成1, 云涛1, 华炯钢1, 付媛1, 张存1,*()   

  1. 1.浙江省农业科学院 畜牧兽医研究所,浙江 杭州 310021
    2.浙江振鸣禽业有限公司,浙江 宁波 315632
    3.河南科技大学 动物科技学院,河南 洛阳 471023
  • 收稿日期:2024-09-09 出版日期:2025-04-25 发布日期:2025-05-09
  • 作者简介:倪征(1979—),女,浙江杭州人,硕士,副研究员,主要从事动物病毒学研究。E-mail:nizheng79@163.com
  • 通讯作者: *张存,E-mail:zhangcun@aliyun.com
  • 基金资助:
    浙江省“尖兵”“领雁”研发攻关计划(2023C02023);2024年度宁海县涉农资金整合项目

Epidemiological investigation and evaluation of purification strategies for avian leukosis in local chicken breeds in Zhejiang Province of China

NI Zheng1(), LU Lei2, ZHU Yinchu1, CHEN Liu1, LI Xinji3, YE Weicheng1, YUN Tao1, HUA Jionggang1, FU Yuan1, ZHANG Cun1,*()   

  1. 1. Institute of Animal Husbandry and Veterinary Medicine, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China
    2. Zhejiang Zhenming Poultry Industry Co., Ltd., Ningbo 315632, Zhejiang, China
    3. College of Veterinary Medicine, Henan University of Science and Technology, Luoyang 471023, Henan, China
  • Received:2024-09-09 Online:2025-04-25 Published:2025-05-09

摘要:

为了解浙江省部分地方品种鸡禽白血病病毒(ALV)的感染情况,并评估禽白血病(AL)净化效果,本研究于2021—2024年采集浙江省6种地方品种鸡的雏鸡胎粪、母鸡蛋清与公鸡血浆样本合计163 453 份,通过检测ALV p27抗原的阳性率分析ALV的感染情况,并进行连续3个世代的AL净化,评估净化效果。同时,对阳性样品进行病毒的分离鉴定和组织病理学检查,通过扩增分离株的囊膜糖蛋白gp85基因,对分离的ALV进行了遗传进化分析。研究结果显示,被调查的6种地方品种鸡均存在 ALV感染,p27抗原的群体平均阳性率为10.02%,其中,白耳黄鸡的阳性率最高,为43.87%。经过3个世代的净化,各样本的阳性率均明显下降,但不同品种的下降幅度差异明显,江山乌骨鸡的净化效果最优,3个世代后蛋清和血浆的阳性率均为0。从阳性样本中分离到2株ALV(ZJU2401和ZJU2402),经进化树分析均属于J亚群。研究结果表明,本净化方案切实可行,研究结果可为浙江省地方品种鸡群AL的净化和防控工作提供参考依据和数据支持。

关键词: 禽白血病, 净化效果评估, gp85基因, 遗传进化

Abstract:

To assess the prevalence of avian leukosis virus (ALV) in local poultry breeds in Zhejiang Province of China and evaluate the effectiveness of a purification program, a comprehensive study was conducted. From 2021 to 2024, a total of 163 453 samples, including meconium, egg white, and plasma, were collected from 6 indigenous poultry breeds in Zhejiang. The presence of ALV p27 antigen was determined using an ELISA kit, and a three-generation purification process was applied to evaluate its efficacy. Positive samples were subjected to isolation, identification, and histopathological examination. The genetic evolution of the isolated ALV strains was analyzed by amplifying the gp85 gene. It was revealed that the ALV infection was prevalent across all 6 local breeds, with an average p27 antigen positive rate of 10.02%. Notably, the white-eared yellow chicken breed exhibited the highest positive rate of 43.87%. Following the three-generation purification process, there was a significant reduction in the positive rate across all samples. However, variations were observed among breeds. The Jiangshan black bone chicken demonstrated the most effective purification, as no detectable p27 antigen in egg white and plasma samples after three generations. Two distinct strains of ALV (ZJU2401 and ZJU2402) were isolated from the positive samples and were classified as subgroup J based on phylogenetic analysis. The study verified that the purification strategy was effective, and these findings could provide a foundation for the management of avian leukosis in local poultry breeds in Zhejiang Province, and offer valuable data support for further purification and preventive measures.

Key words: avian leukosis, evaluation of purification effect, gp85 gene, genetic evolution

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