浙江农业学报 ›› 2025, Vol. 37 ›› Issue (4): 790-799.DOI: 10.3969/j.issn.1004-1524.20240792
倪征1(), 卢磊2, 朱寅初1, 陈柳1, 李鑫基3, 叶伟成1, 云涛1, 华炯钢1, 付媛1, 张存1,*(
)
收稿日期:
2024-09-09
出版日期:
2025-04-25
发布日期:
2025-05-09
作者简介:
倪征(1979—),女,浙江杭州人,硕士,副研究员,主要从事动物病毒学研究。E-mail:nizheng79@163.com
通讯作者:
*张存,E-mail:zhangcun@aliyun.com
基金资助:
NI Zheng1(), LU Lei2, ZHU Yinchu1, CHEN Liu1, LI Xinji3, YE Weicheng1, YUN Tao1, HUA Jionggang1, FU Yuan1, ZHANG Cun1,*(
)
Received:
2024-09-09
Online:
2025-04-25
Published:
2025-05-09
摘要:
为了解浙江省部分地方品种鸡禽白血病病毒(ALV)的感染情况,并评估禽白血病(AL)净化效果,本研究于2021—2024年采集浙江省6种地方品种鸡的雏鸡胎粪、母鸡蛋清与公鸡血浆样本合计163 453 份,通过检测ALV p27抗原的阳性率分析ALV的感染情况,并进行连续3个世代的AL净化,评估净化效果。同时,对阳性样品进行病毒的分离鉴定和组织病理学检查,通过扩增分离株的囊膜糖蛋白gp85基因,对分离的ALV进行了遗传进化分析。研究结果显示,被调查的6种地方品种鸡均存在 ALV感染,p27抗原的群体平均阳性率为10.02%,其中,白耳黄鸡的阳性率最高,为43.87%。经过3个世代的净化,各样本的阳性率均明显下降,但不同品种的下降幅度差异明显,江山乌骨鸡的净化效果最优,3个世代后蛋清和血浆的阳性率均为0。从阳性样本中分离到2株ALV(ZJU2401和ZJU2402),经进化树分析均属于J亚群。研究结果表明,本净化方案切实可行,研究结果可为浙江省地方品种鸡群AL的净化和防控工作提供参考依据和数据支持。
中图分类号:
倪征, 卢磊, 朱寅初, 陈柳, 李鑫基, 叶伟成, 云涛, 华炯钢, 付媛, 张存. 浙江省地方品种鸡禽白血病的流行病学调查与净化策略评估[J]. 浙江农业学报, 2025, 37(4): 790-799.
NI Zheng, LU Lei, ZHU Yinchu, CHEN Liu, LI Xinji, YE Weicheng, YUN Tao, HUA Jionggang, FU Yuan, ZHANG Cun. Epidemiological investigation and evaluation of purification strategies for avian leukosis in local chicken breeds in Zhejiang Province of China[J]. Acta Agriculturae Zhejiangensis, 2025, 37(4): 790-799.
毒株Strain | 亚群Subgroup | 登录号GenBank accession No. | 年份Year | 地域Area |
---|---|---|---|---|
NX0101 | J | DQ115805 | 2016 | 中国China |
NGA_VX23 | J | MK104146 | 2018 | 尼日利亚Nigeria |
J-SDAU12-737 | J | KM873195 | 2015 | 中国China |
GDQJ-4 | J | KU170199 | 2016 | 中国China |
HPRS103 | J | Z46390 | 2006 | 英国UK |
GD1804J6 | J | MZ856317 | 2021 | 中国China |
JS17DX01 | K | MT783282 | 2021 | 中国China |
SD20LH01 | K | OP035379 | 2023 | 中国China |
CH/JXND2101 | K | OR670961 | 2024 | 中国China |
CH/JXTH2202 | K | OR670967 | 2024 | 中国China |
JS13LY19 | K | MG770235 | 2018 | 中国China |
JS11C1 | K | KF746200 | 2014 | 中国China |
JS13-DX5 | K | KF999961 | 2014 | 中国China |
MQNCSU | A | DQ365814 | 2016 | 英国UK |
RAV-1 | A | MF926337 | 2019 | 美国USA |
SDAU09E1 | A | HM452341 | 2016 | 中国China |
SDAU09E2 | A | HM452342 | 2016 | 中国China |
DPRE32 | A | KM434201 | 2014 | 印度India |
SDAU09E3 | B | JF826241 | 2012 | 中国China |
SDAU09C2 | B | HM446005 | 2016 | 中国China |
GX14FF03 | B | KU923579 | 2016 | 中国China |
HLJA0202 | B | MW804649 | 2022 | 中国China |
ALVE-B10 | E | KC610516 | 2013 | 加拿大Canada |
ev-21 | E | KY235336| | 2016 | 中国China |
AF229 | E | MF817821 | 2016 | 美国USA |
RAV0 | E | MF817822 | 2017 | 美国USA |
ev-I | E | AY013303 | 2001 | 美国USA |
SDO501 | E | EF467236 | 2007 | 中国China |
表1 ALV各亚群的参考毒株信息
Table 1 Information of reference strains for different subgroups of ALV
毒株Strain | 亚群Subgroup | 登录号GenBank accession No. | 年份Year | 地域Area |
---|---|---|---|---|
NX0101 | J | DQ115805 | 2016 | 中国China |
NGA_VX23 | J | MK104146 | 2018 | 尼日利亚Nigeria |
J-SDAU12-737 | J | KM873195 | 2015 | 中国China |
GDQJ-4 | J | KU170199 | 2016 | 中国China |
HPRS103 | J | Z46390 | 2006 | 英国UK |
GD1804J6 | J | MZ856317 | 2021 | 中国China |
JS17DX01 | K | MT783282 | 2021 | 中国China |
SD20LH01 | K | OP035379 | 2023 | 中国China |
CH/JXND2101 | K | OR670961 | 2024 | 中国China |
CH/JXTH2202 | K | OR670967 | 2024 | 中国China |
JS13LY19 | K | MG770235 | 2018 | 中国China |
JS11C1 | K | KF746200 | 2014 | 中国China |
JS13-DX5 | K | KF999961 | 2014 | 中国China |
MQNCSU | A | DQ365814 | 2016 | 英国UK |
RAV-1 | A | MF926337 | 2019 | 美国USA |
SDAU09E1 | A | HM452341 | 2016 | 中国China |
SDAU09E2 | A | HM452342 | 2016 | 中国China |
DPRE32 | A | KM434201 | 2014 | 印度India |
SDAU09E3 | B | JF826241 | 2012 | 中国China |
SDAU09C2 | B | HM446005 | 2016 | 中国China |
GX14FF03 | B | KU923579 | 2016 | 中国China |
HLJA0202 | B | MW804649 | 2022 | 中国China |
ALVE-B10 | E | KC610516 | 2013 | 加拿大Canada |
ev-21 | E | KY235336| | 2016 | 中国China |
AF229 | E | MF817821 | 2016 | 美国USA |
RAV0 | E | MF817822 | 2017 | 美国USA |
ev-I | E | AY013303 | 2001 | 美国USA |
SDO501 | E | EF467236 | 2007 | 中国China |
品种 Variety | 日龄 Age/d | 阳性率Positive rate/% | ||
---|---|---|---|---|
公鸡Rooster | 母鸡Hen | 平均Averagel | ||
仙居鸡Xianju chicken | 240 | 10.75(20/186) | 2.71 (33/1 209) | 3.80 (53/1 395) |
萧山鸡Xiaoshan chicken | 220 | 6.91 (15/217) | 6.65 (104/1 564) | 6.68 (119/1 781) |
江山乌骨鸡Jiangshan silky chicken | 210 | 2.50 (2/80) | 3.85 (7/182) | 3.44 (9/262) |
矮脚黄鸡Aijiao yellow chicken | 220 | 20.95 (22/105) | 11.60 (110/948) | 12.54 (132/1 053) |
麻黄土鸡Mahuang indigenous chicken | 225 | 6.33 (5/79) | 10.42 (45/432) | 9.78 (50/511) |
白耳黄鸡Baier yellow chicken | 240 | 47.13 (41/87) | 42.99 (138/321) | 43.87 (179/408) |
合计Total | _ | 13.93 (105/754) | 9.39 (437/4 656) | 10.02 (542/5 410) |
表2 浙江省不同地方品种鸡的ALV阳性率
Table 2 ALV positive rates of local chicken breeds from different regions of Zhejiang Province
品种 Variety | 日龄 Age/d | 阳性率Positive rate/% | ||
---|---|---|---|---|
公鸡Rooster | 母鸡Hen | 平均Averagel | ||
仙居鸡Xianju chicken | 240 | 10.75(20/186) | 2.71 (33/1 209) | 3.80 (53/1 395) |
萧山鸡Xiaoshan chicken | 220 | 6.91 (15/217) | 6.65 (104/1 564) | 6.68 (119/1 781) |
江山乌骨鸡Jiangshan silky chicken | 210 | 2.50 (2/80) | 3.85 (7/182) | 3.44 (9/262) |
矮脚黄鸡Aijiao yellow chicken | 220 | 20.95 (22/105) | 11.60 (110/948) | 12.54 (132/1 053) |
麻黄土鸡Mahuang indigenous chicken | 225 | 6.33 (5/79) | 10.42 (45/432) | 9.78 (50/511) |
白耳黄鸡Baier yellow chicken | 240 | 47.13 (41/87) | 42.99 (138/321) | 43.87 (179/408) |
合计Total | _ | 13.93 (105/754) | 9.39 (437/4 656) | 10.02 (542/5 410) |
品种 Variety | 样品类型 Sample type | 阳性率Positive rate/% | ||
---|---|---|---|---|
1世代Generation 1 | 2世代Generation 2 | 3世代Generation 3 | ||
仙居鸡 | 胎粪Meconium | 3.36(175/5 215) | 2.38(118/4 968) | 0.52(26/5 026) |
Xianju chicken | 蛋清Egg white | 1.29(24/1 858) | 0.32(6/1 882) | 0.58(7/1 212) |
血浆Plasma | 3.96(72/1 818) | 1.08(16/1 480) | 0.57(9/1 584) | |
萧山鸡 | 胎粪Meconium | 5.39(336/6 237) | 1.58(95/6 031) | 1.67(100/5 977) |
Xiaoshan chicken | 蛋清Egg white | 2.20(60/2 727) | 0.68(12/1 772) | 0.07(1/1 460) |
血浆Plasma | 3.13(60/1 920) | 4.68(80/1 710) | 1.44(26/1 807) | |
江山乌骨鸡 | 胎粪Meconium | 3.17(175/5 527) | 2.18(116/5 324) | 0.20(11/5 621) |
Jiangshan silky chicken | 蛋清Egg white | 3.54(64/1 808) | 1.72(32/1 864) | 0(0/2 020) |
血浆Plasma | 1.61(26/1 612) | 0(0/1 550) | 0(0/1 920) | |
矮脚黄鸡 | 胎粪Meconium | 15.42(931/6 036) | 6.97(430/6 167) | 2.56(156/6 085) |
Aijiao yellow chicken | 蛋清Egg white | 5.36(102/1 902) | 2.19(44/2 012) | 1.87(24/1 283) |
血浆Plasma | 17.78(312/1 755) | 7.36(132/1 793) | 4.55(80/1 760) | |
麻黄土鸡 | 胎粪Meconium | 7.68(449/5 843) | 5.98(333/5 573) | 0.33(18/5 396) |
Mahuang indigenous chicken | 蛋清Egg white | 8.44(128/1 516) | 4.70(85/1 810) | 0.31(6/1 938) |
血浆Plasma | 5.00(63/1 260) | 4.35(60/1 380) | 1.28(20/1 560) | |
白耳黄鸡 | 胎粪Meconium | 18.80(1059/5 634) | 10.13(597/5 894) | 1.21(69/5 703) |
Baier yellow chicken | 蛋清Egg white | 14.47(245/1 701) | 8.85(132/1 548) | 1.85(33/1 782) |
血浆Plasma | 3.80(54/1 422) | 2.27(30/1 320) | 0(0/1 450) |
表3 6个地方品种鸡群3个世代禽白血病净化结果
Table 3 Purification results of three generations of avian leukemia in six local chicken breeds
品种 Variety | 样品类型 Sample type | 阳性率Positive rate/% | ||
---|---|---|---|---|
1世代Generation 1 | 2世代Generation 2 | 3世代Generation 3 | ||
仙居鸡 | 胎粪Meconium | 3.36(175/5 215) | 2.38(118/4 968) | 0.52(26/5 026) |
Xianju chicken | 蛋清Egg white | 1.29(24/1 858) | 0.32(6/1 882) | 0.58(7/1 212) |
血浆Plasma | 3.96(72/1 818) | 1.08(16/1 480) | 0.57(9/1 584) | |
萧山鸡 | 胎粪Meconium | 5.39(336/6 237) | 1.58(95/6 031) | 1.67(100/5 977) |
Xiaoshan chicken | 蛋清Egg white | 2.20(60/2 727) | 0.68(12/1 772) | 0.07(1/1 460) |
血浆Plasma | 3.13(60/1 920) | 4.68(80/1 710) | 1.44(26/1 807) | |
江山乌骨鸡 | 胎粪Meconium | 3.17(175/5 527) | 2.18(116/5 324) | 0.20(11/5 621) |
Jiangshan silky chicken | 蛋清Egg white | 3.54(64/1 808) | 1.72(32/1 864) | 0(0/2 020) |
血浆Plasma | 1.61(26/1 612) | 0(0/1 550) | 0(0/1 920) | |
矮脚黄鸡 | 胎粪Meconium | 15.42(931/6 036) | 6.97(430/6 167) | 2.56(156/6 085) |
Aijiao yellow chicken | 蛋清Egg white | 5.36(102/1 902) | 2.19(44/2 012) | 1.87(24/1 283) |
血浆Plasma | 17.78(312/1 755) | 7.36(132/1 793) | 4.55(80/1 760) | |
麻黄土鸡 | 胎粪Meconium | 7.68(449/5 843) | 5.98(333/5 573) | 0.33(18/5 396) |
Mahuang indigenous chicken | 蛋清Egg white | 8.44(128/1 516) | 4.70(85/1 810) | 0.31(6/1 938) |
血浆Plasma | 5.00(63/1 260) | 4.35(60/1 380) | 1.28(20/1 560) | |
白耳黄鸡 | 胎粪Meconium | 18.80(1059/5 634) | 10.13(597/5 894) | 1.21(69/5 703) |
Baier yellow chicken | 蛋清Egg white | 14.47(245/1 701) | 8.85(132/1 548) | 1.85(33/1 782) |
血浆Plasma | 3.80(54/1 422) | 2.27(30/1 320) | 0(0/1 450) |
图1 不同地方品种鸡的净化结果 1,仙居鸡;2,萧山鸡;3,江山乌骨鸡;4,矮脚黄鸡;5,麻黄土鸡;6,白耳黄鸡。A,公鸡ALV P27抗原阳性率;B,母鸡ALV P27抗原阳性率;C,雏鸡ALV P27抗原阳性率。
Fig.1 Purification results of different local chicken breeds 1, Xianju chicken; 2, Xiaoshan chicken; 3, Jiangshan silky chicken; 4, Aijiao yellow chicken; 5, Mahuang indigenous chicken; 6, Baier yellow chicken. A, ALV P27 antigen positive rates in roosters; B, ALV P27 antigen Positive rates in hens; C, ALV P27 antigen Positive rates in chicks.
图4 ALV阳性感染鸡不同组织的病理变化(HE染色) A,脾脏;B,肺脏;C,肝脏;D,盲肠扁桃体。
Fig.4 Pathological changes of different tissues with infected pigs (HE staining) A, Spleen; B, Lung; C, Liver; D, Cecal tonsils.
图5 ALV分离毒株gp85基因的PCR鉴定结果 M,DL2 000 DNA Marker;1,ZJU2401;2, ZJU2402;3, 阳性对照;4,阴性对照。
Fig.5 PCR identification of gp85 gene in ALV isolated strains M, DL2 000 DNA Marker; 1, ZJU2401; 2, ZJU2402; 3, Positive control; 4, Negative control.
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