浙江省地方品种鸡禽白血病的流行病学调查与净化策略评估

doi:10.3969/j.issn.1004-1524.20240792

浙江农业学报 ›› 2025, Vol. 37 ›› Issue (4): 790-799.DOI: 10.3969/j.issn.1004-1524.20240792

浙江省地方品种鸡禽白血病的流行病学调查与净化策略评估

倪征¹(), 卢磊², 朱寅初¹, 陈柳¹, 李鑫基³, 叶伟成¹, 云涛¹, 华炯钢¹, 付媛¹, 张存¹^,^*()

1.浙江省农业科学院畜牧兽医研究所,浙江杭州 310021
2.浙江振鸣禽业有限公司,浙江宁波 315632
3.河南科技大学动物科技学院,河南洛阳 471023

收稿日期:2024-09-09 出版日期:2025-04-25 发布日期:2025-05-09
作者简介:倪征(1979—),女,浙江杭州人,硕士,副研究员,主要从事动物病毒学研究。E-mail:nizheng79@163.com
通讯作者: *张存,E-mail:zhangcun@aliyun.com
基金资助:
浙江省“尖兵”“领雁”研发攻关计划(2023C02023);2024年度宁海县涉农资金整合项目

Epidemiological investigation and evaluation of purification strategies for avian leukosis in local chicken breeds in Zhejiang Province of China

NI Zheng¹(), LU Lei², ZHU Yinchu¹, CHEN Liu¹, LI Xinji³, YE Weicheng¹, YUN Tao¹, HUA Jionggang¹, FU Yuan¹, ZHANG Cun¹^,^*()

1. Institute of Animal Husbandry and Veterinary Medicine, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China
2. Zhejiang Zhenming Poultry Industry Co., Ltd., Ningbo 315632, Zhejiang, China
3. College of Veterinary Medicine, Henan University of Science and Technology, Luoyang 471023, Henan, China

Received:2024-09-09 Online:2025-04-25 Published:2025-05-09

摘要/Abstract

摘要：

为了解浙江省部分地方品种鸡禽白血病病毒(ALV)的感染情况,并评估禽白血病(AL)净化效果,本研究于2021—2024年采集浙江省6种地方品种鸡的雏鸡胎粪、母鸡蛋清与公鸡血浆样本合计163 453 份,通过检测ALV p27抗原的阳性率分析ALV的感染情况,并进行连续3个世代的AL净化,评估净化效果。同时,对阳性样品进行病毒的分离鉴定和组织病理学检查,通过扩增分离株的囊膜糖蛋白gp85基因,对分离的ALV进行了遗传进化分析。研究结果显示,被调查的6种地方品种鸡均存在 ALV感染,p27抗原的群体平均阳性率为10.02%,其中,白耳黄鸡的阳性率最高,为43.87%。经过3个世代的净化,各样本的阳性率均明显下降,但不同品种的下降幅度差异明显,江山乌骨鸡的净化效果最优,3个世代后蛋清和血浆的阳性率均为0。从阳性样本中分离到2株ALV(ZJU2401和ZJU2402),经进化树分析均属于J亚群。研究结果表明,本净化方案切实可行,研究结果可为浙江省地方品种鸡群AL的净化和防控工作提供参考依据和数据支持。

关键词: 禽白血病, 净化效果评估, gp85基因, 遗传进化

Abstract:

To assess the prevalence of avian leukosis virus (ALV) in local poultry breeds in Zhejiang Province of China and evaluate the effectiveness of a purification program, a comprehensive study was conducted. From 2021 to 2024, a total of 163 453 samples, including meconium, egg white, and plasma, were collected from 6 indigenous poultry breeds in Zhejiang. The presence of ALV p27 antigen was determined using an ELISA kit, and a three-generation purification process was applied to evaluate its efficacy. Positive samples were subjected to isolation, identification, and histopathological examination. The genetic evolution of the isolated ALV strains was analyzed by amplifying the gp85 gene. It was revealed that the ALV infection was prevalent across all 6 local breeds, with an average p27 antigen positive rate of 10.02%. Notably, the white-eared yellow chicken breed exhibited the highest positive rate of 43.87%. Following the three-generation purification process, there was a significant reduction in the positive rate across all samples. However, variations were observed among breeds. The Jiangshan black bone chicken demonstrated the most effective purification, as no detectable p27 antigen in egg white and plasma samples after three generations. Two distinct strains of ALV (ZJU2401 and ZJU2402) were isolated from the positive samples and were classified as subgroup J based on phylogenetic analysis. The study verified that the purification strategy was effective, and these findings could provide a foundation for the management of avian leukosis in local poultry breeds in Zhejiang Province, and offer valuable data support for further purification and preventive measures.

Key words: avian leukosis, evaluation of purification effect, gp85 gene, genetic evolution

中图分类号:

S852.659.1

倪征, 卢磊, 朱寅初, 陈柳, 李鑫基, 叶伟成, 云涛, 华炯钢, 付媛, 张存. 浙江省地方品种鸡禽白血病的流行病学调查与净化策略评估[J]. 浙江农业学报, 2025, 37(4): 790-799.

NI Zheng, LU Lei, ZHU Yinchu, CHEN Liu, LI Xinji, YE Weicheng, YUN Tao, HUA Jionggang, FU Yuan, ZHANG Cun. Epidemiological investigation and evaluation of purification strategies for avian leukosis in local chicken breeds in Zhejiang Province of China[J]. Acta Agriculturae Zhejiangensis, 2025, 37(4): 790-799.

图/表 9

参考文献 26

[1]	刘金华, 甘孟侯. 中国禽病学[M]. 2版. 北京: 中国农业出版社, 2016: 77-81.
[2]	CHEN J, LI J Q, DONG X Y, et al. The key amino acid sites 199-205, 269, 319, 321 and 324 of ALV-K env contribute to the weaker replication capacity of ALV-K than ALV-A[J]. Retrovirology, 2022, 19(1): 19.
[3]	俞燕. 地方品种鸡群禽白血病流行病学调查及检测净化技术研究[D]. 扬州: 扬州大学, 2019.
	YU Y. Epidemiological investigation of aian leukosis in chinese local chicken breeds and research on detection and eradication technology of exogenous ALV[D]. Yangzhou: Yangzhou University, 2019.
[4]	WANG P K, LIN L L, SHI M Y, et al. Vertical transmission of ALV from ALV-J positive parents caused severe immunosuppression and significantly reduced Marek’s disease vaccine efficacy in three-yellow chickens[J]. Veterinary Microbiology, 2020, 244: 108683.
[5]	LI H J, WANG P K, LIN L L, et al. The emergence of the infection of subgroup J avian leucosis virus escalated the tumour incidence in commercial Yellow chickens in Southern China in recent years[J]. Transboundary and Emerging Diseases, 2019, 66(1): 312-316.
[6]	王鑫, 赵鹏, 崔治中. 我国地方品种鸡分离到的一个禽白血病病毒新亚群的鉴定[J]. 病毒学报, 2012, 28(6): 609-614.
	WANG X, ZHAO P, CUI Z Z. Identification of a new subgroup of avian leukosis virus isolated from Chinese indigenous chicken breeds[J]. Chinese Journal of Virology, 2012, 28(6): 609-614. (in Chinese with English abstract)
[7]	SWAYNE D E, GLISSON J R, MCDOUGALD L R, et al. Diseases of poultry.[M]. 13th ed. Hoboken: Wiley-Blackwell, 2013: 553-592.
[8]	CURRISTIN S M, BIRD K J, TUBBS R J, et al. VBP and RelA regulate avian leukosis virus long terminal repeat-enhanced transcription in B cells[J]. Journal of Virology, 1997, 71(8): 5972-5981.
[9]	VENUGOPAL K, HOWES K, BARRON G S, et al. Recombinant env-gp85 of HPRS-103 (subgroup J) avian leukosis virus: antigenic characteristics and usefulness as a diagnostic reagent[J]. Avian Diseases, 1997, 41(2): 283-288.
[10]	JUSTICE J IV, BEEMON K L. Avian retroviral replication[J]. Current Opinion in Virology, 2013, 3(6): 664-669.
[11]	刘青, 成子强. 禽白血病病毒跨膜蛋白在膜融合及免疫抑制中的作用[J]. 动物医学进展, 2008, 29(10): 82-85.
	LIU Q, CHENG Z Q. The Role of avian leukosis virus thansmembrane protein in membrane fusion and immunosuppression[J]. Progress in Veterinary Medicine, 2008, 29(10): 82-85. (in Chinese with English abstract)
[12]	PAYNE L N, BROWN S R, BUMSTEAD N, et al. A novel subgroup of exogenous avian leukosis virus in chickens[J]. The Journal of General Virology, 1991, 72 (4): 801-807.
[13]	FENG M, ZHANG X Q. Immunity to avian leukosis virus: where are we now and what should we do?[J]. Frontiers in Immunology, 2016, 7: 624.
[14]	SU Q, LI Y, CUI Z Z, et al. The emerging novel avian leukosis virus with mutations in the pol gene shows competitive replication advantages both in vivo and in vitro[J]. Emerging Microbes & Infections, 2018, 7(1): 117.
[15]	ZHAO Z J, RAO M Z, LIAO M, et al. Phylogenetic analysis and pathogenicity assessment of the emerging recombinant subgroup K of avian leukosis virus in South China[J]. Viruses, 2018, 10(4): 194.
[16]	FADLY A M. Isolation and identification of avian leukosis viruses: a review[J]. Avian Pathology, 2000, 29(6): 529-535.
[17]	PAYNE L N, HOWES K, GILLESPIE A M, et al. Host range of Rous sarcoma virus pseudotype RSV(HPRS-103) in 12 avian species: support for a new avian retrovirus envelope subgroup, designated J[J]. The Journal of General Virology, 1992, 73 (11): 2995-2997.
[18]	崔治中. 一种新的鸡白血病病毒[J]. 动物科学与动物医学, 1999, 16 (2): 2-3.
	CUI Z Z. A novel chicken leukemia virus[J]. Animal Science and Animal medicine, 1999, 16 (2): 2-3.
[19]	SU Q, LI Y, LI W H, et al. Molecular characteristics of avian leukosis viruses isolated from indigenous chicken breeds in China[J]. Poultry Science, 2018, 97(8): 2917-2925.
[20]	MENG F F, LI Q C, ZHANG Y W, et al. Characterization of subgroup J avian Leukosis virus isolated from Chinese indigenous chickens[J]. Virology Journal, 2018, 15(1): 33.
[21]	LIANG X Y, GU Y F, CHEN X Y, et al. Identification and characterization of a novel natural recombinant avian leucosis virus from Chinese indigenous chicken flock[J]. Virus Genes, 2019, 55(5): 726-733.
[22]	成子强, 刘思当, 孟祥凯, 等. 商品蛋鸡成髓细胞瘤、血管瘤型J亚群白血病病理学初报[J]. 畜牧兽医学报, 2008, 39(7): 935-940.
	CHENG Z Q, LIU S D, MENG X K, et al. Preliminary pathology report of myeloblastosis and hemangioma associated with ALV-J in commercial layer chicken[J]. Chinese Journal of Animal and Veterinary Sciences, 2008, 39(7): 935-940. (in Chinese with English abstract)
[23]	王培坤, 泰丽莉, 毕玉彧, 等. 广西麻鸡感染A亚群与J亚群禽白血病病毒的诊断[J]. 中国家禽, 2015, 37(5): 56-58.
	WANG P K, QIN L L, BI Y Y, et al. Diagnosis of avian leukemia virus infected with subgroup A and subgroup J in Guangxi hemp chickens[J]. China Poultry, 2015, 37(5):56-58.
[24]	杨荣坤, 许青荣, 崔卫涛, 等. 地方鸡禽白血病病毒感染调查及主要流行亚群分析[J]. 华中农业大学学报, 2020, 39(3): 89-93.
	YANG R K, XU Q R, CUI W T, et al. Investigation of avian leukosis virus infection and analysis of main epidemic subgroups in indigenous chicken[J]. Journal of Huazhong Agricultural University, 2020, 39(3): 89-93. (in Chinese with English abstract)
[25]	张倩. 我国祖代种鸡场禽白血病、禽网状内皮组织增殖症和鸡白痢的监测与分析[D]. 北京: 中国农业大学, 2017.
	ZHANG Q. Surveillance of avian leucosis, reticuloendotheliosis, pullorum disease in crandparent breeding farms of China[D]. Beijing: China Agricultural University, 2017. (in Chinese with English abstract)
[26]	赵胜杰, 冉晓龙, 盛敏. 河南省规模鸡场禽白血病感染情况调查[J]. 中国畜禽种业, 2023, 19(1): 95-96.
	ZHAO S J, RAN X L, SHENG M. Investigation on the infection status of avian autohemosis in Henan province[J]. The Chinese Livestock and Poultry Breeding, 2023, 19(1): 95-96.

毒株Strain	亚群Subgroup	登录号GenBank accession No.	年份Year	地域Area
NX0101	J	DQ115805	2016	中国China
NGA_VX23	J	MK104146	2018	尼日利亚Nigeria
J-SDAU12-737	J	KM873195	2015	中国China
GDQJ-4	J	KU170199	2016	中国China
HPRS103	J	Z46390	2006	英国UK
GD1804J6	J	MZ856317	2021	中国China
JS17DX01	K	MT783282	2021	中国China
SD20LH01	K	OP035379	2023	中国China
CH/JXND2101	K	OR670961	2024	中国China
CH/JXTH2202	K	OR670967	2024	中国China
JS13LY19	K	MG770235	2018	中国China
JS11C1	K	KF746200	2014	中国China
JS13-DX5	K	KF999961	2014	中国China
MQNCSU	A	DQ365814	2016	英国UK
RAV-1	A	MF926337	2019	美国USA
SDAU09E1	A	HM452341	2016	中国China
SDAU09E2	A	HM452342	2016	中国China
DPRE32	A	KM434201	2014	印度India
SDAU09E3	B	JF826241	2012	中国China
SDAU09C2	B	HM446005	2016	中国China
GX14FF03	B	KU923579	2016	中国China
HLJA0202	B	MW804649	2022	中国China
ALVE-B10	E	KC610516	2013	加拿大Canada
ev-21	E	KY235336\|	2016	中国China
AF229	E	MF817821	2016	美国USA
RAV0	E	MF817822	2017	美国USA
ev-I	E	AY013303	2001	美国USA
SDO501	E	EF467236	2007	中国China

毒株Strain	亚群Subgroup	登录号GenBank accession No.	年份Year	地域Area
NX0101	J	DQ115805	2016	中国China
NGA_VX23	J	MK104146	2018	尼日利亚Nigeria
J-SDAU12-737	J	KM873195	2015	中国China
GDQJ-4	J	KU170199	2016	中国China
HPRS103	J	Z46390	2006	英国UK
GD1804J6	J	MZ856317	2021	中国China
JS17DX01	K	MT783282	2021	中国China
SD20LH01	K	OP035379	2023	中国China
CH/JXND2101	K	OR670961	2024	中国China
CH/JXTH2202	K	OR670967	2024	中国China
JS13LY19	K	MG770235	2018	中国China
JS11C1	K	KF746200	2014	中国China
JS13-DX5	K	KF999961	2014	中国China
MQNCSU	A	DQ365814	2016	英国UK
RAV-1	A	MF926337	2019	美国USA
SDAU09E1	A	HM452341	2016	中国China
SDAU09E2	A	HM452342	2016	中国China
DPRE32	A	KM434201	2014	印度India
SDAU09E3	B	JF826241	2012	中国China
SDAU09C2	B	HM446005	2016	中国China
GX14FF03	B	KU923579	2016	中国China
HLJA0202	B	MW804649	2022	中国China
ALVE-B10	E	KC610516	2013	加拿大Canada
ev-21	E	KY235336\|	2016	中国China
AF229	E	MF817821	2016	美国USA
RAV0	E	MF817822	2017	美国USA
ev-I	E	AY013303	2001	美国USA
SDO501	E	EF467236	2007	中国China

品种 Variety	日龄 Age/d	阳性率Positive rate/%
品种 Variety	日龄 Age/d	公鸡Rooster	母鸡Hen	平均Averagel
仙居鸡Xianju chicken	240	10.75(20/186)	2.71 (33/1 209)	3.80 (53/1 395)
萧山鸡Xiaoshan chicken	220	6.91 (15/217)	6.65 (104/1 564)	6.68 (119/1 781)
江山乌骨鸡Jiangshan silky chicken	210	2.50 (2/80)	3.85 (7/182)	3.44 (9/262)
矮脚黄鸡Aijiao yellow chicken	220	20.95 (22/105)	11.60 (110/948)	12.54 (132/1 053)
麻黄土鸡Mahuang indigenous chicken	225	6.33 (5/79)	10.42 (45/432)	9.78 (50/511)
白耳黄鸡Baier yellow chicken	240	47.13 (41/87)	42.99 (138/321)	43.87 (179/408)
合计Total	_	13.93 (105/754)	9.39 (437/4 656)	10.02 (542/5 410)

品种 Variety	日龄 Age/d	阳性率Positive rate/%
品种 Variety	日龄 Age/d	公鸡Rooster	母鸡Hen	平均Averagel
仙居鸡Xianju chicken	240	10.75(20/186)	2.71 (33/1 209)	3.80 (53/1 395)
萧山鸡Xiaoshan chicken	220	6.91 (15/217)	6.65 (104/1 564)	6.68 (119/1 781)
江山乌骨鸡Jiangshan silky chicken	210	2.50 (2/80)	3.85 (7/182)	3.44 (9/262)
矮脚黄鸡Aijiao yellow chicken	220	20.95 (22/105)	11.60 (110/948)	12.54 (132/1 053)
麻黄土鸡Mahuang indigenous chicken	225	6.33 (5/79)	10.42 (45/432)	9.78 (50/511)
白耳黄鸡Baier yellow chicken	240	47.13 (41/87)	42.99 (138/321)	43.87 (179/408)
合计Total	_	13.93 (105/754)	9.39 (437/4 656)	10.02 (542/5 410)

品种 Variety	样品类型 Sample type	阳性率Positive rate/%
品种 Variety	样品类型 Sample type	1世代Generation 1	2世代Generation 2	3世代Generation 3
仙居鸡	胎粪Meconium	3.36(175/5 215)	2.38(118/4 968)	0.52(26/5 026)
Xianju chicken	蛋清Egg white	1.29(24/1 858)	0.32(6/1 882)	0.58(7/1 212)
	血浆Plasma	3.96(72/1 818)	1.08(16/1 480)	0.57(9/1 584)
萧山鸡	胎粪Meconium	5.39(336/6 237)	1.58(95/6 031)	1.67(100/5 977)
Xiaoshan chicken	蛋清Egg white	2.20(60/2 727)	0.68(12/1 772)	0.07(1/1 460)
	血浆Plasma	3.13(60/1 920)	4.68(80/1 710)	1.44(26/1 807)
江山乌骨鸡	胎粪Meconium	3.17(175/5 527)	2.18(116/5 324)	0.20(11/5 621)
Jiangshan silky chicken	蛋清Egg white	3.54(64/1 808)	1.72(32/1 864)	0(0/2 020)
	血浆Plasma	1.61(26/1 612)	0(0/1 550)	0(0/1 920)
矮脚黄鸡	胎粪Meconium	15.42(931/6 036)	6.97(430/6 167)	2.56(156/6 085)
Aijiao yellow chicken	蛋清Egg white	5.36(102/1 902)	2.19(44/2 012)	1.87(24/1 283)
	血浆Plasma	17.78(312/1 755)	7.36(132/1 793)	4.55(80/1 760)
麻黄土鸡	胎粪Meconium	7.68(449/5 843)	5.98(333/5 573)	0.33(18/5 396)
Mahuang indigenous chicken	蛋清Egg white	8.44(128/1 516)	4.70(85/1 810)	0.31(6/1 938)
	血浆Plasma	5.00(63/1 260)	4.35(60/1 380)	1.28(20/1 560)
白耳黄鸡	胎粪Meconium	18.80(1059/5 634)	10.13(597/5 894)	1.21(69/5 703)
Baier yellow chicken	蛋清Egg white	14.47(245/1 701)	8.85(132/1 548)	1.85(33/1 782)
	血浆Plasma	3.80(54/1 422)	2.27(30/1 320)	0(0/1 450)

浙江省地方品种鸡禽白血病的流行病学调查与净化策略评估

Epidemiological investigation and evaluation of purification strategies for avian leukosis in local chicken breeds in Zhejiang Province of China

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[7]	李雨濛, 郭博, 徐逸飞, 陈瑛琪, 蔡瑶, 李小璟, 龚双燕, 徐志文, 朱玲. 四川省腹泻病猪的猪萨佩罗病毒检测及1B基因序列比较分析[J]. 浙江农业学报, 2016, 28(12): 1992-1999.
[8]	秦卓明1，3，徐怀英1，马秀丽1，，黄兵1，李玉峰1，李福伟1，于可响1，崔治中2，. 从芦花鸡分离的J亚群ALV病毒及其gp85基因演化 [J]. 浙江农业学报, 2014, 26(5): 1180-.