关键词: 邻苯二甲酸二甲酯（DMP）, 黑土, PAEs污染, 降解菌, 正交优化

Abstract:  Dimethyl phthalate （DMP）， a toxic organic compound， is ubiquitous environmental pollutant， and it has been listed as a priority pollutants by China State Environmental Protection Administration （SEPA）. In this study， one strain was isolated and identified from the black soils， which was covered by the film for long time. The characteristics of morphology and 16S rDNA sequence analysis revealed that this strain was classified as Bacillus sp. and named as QD\|9\|10. The further study indicated that this strain could utilize DMP as carbon source for growth. To optimize the degradation conditions， an orthogonal experiment was designed. The results showed that the optimized conditions were：pH 80， temperature 350℃， shaking speed 1240 r·min-1， and DMP 1004 mg·L-1. The studies on the characteristics of their growth indicated that DMP could be degraded completely in 32 h while the concenration of DMP was 100 mg·L-1 in the medium. In the meantime， substrate utilization tests showed that QD\|9\|10 strain could utilize other phthalic acid ester （PAE） pollutants， such as DBP， DEP and DEHP. Therefore， it was expected that the strain QD\|9\|10 could be used to clear PAEs pollution in future.

Key words: dimethyl phthalate, black soil, PAEs pollution, degradation bacteria, orthogonal optimize test