关键词: 猪α\, 干扰素, 双抗体夹心ELISA, 定量检测

Abstract: In order to accurately measure the concentration of porcine α\|interferon (PoIFN\|α) in swine serum and PoIFN\|α product. Double antibody sandwich ELISA (DAS\|ELISA) method was developed by using purified rabbit anti PoIFN\|α IgG as capture antibody, and purified mouse anti PoIFN\|α IgG as detection antibody. The result indicated that the best package concentration of rabbit anti porcine PoIFN\|α IgG was 125 μg·mL-1, and mouse anti PoIFN\|α IgG was 625 μg·mL-1. The detection range of the DAS\|ELISA was between 0078 1-250 μg·mL-1 by standard curve established using the known concentration of standard protein of PoIFN\|α. Compared with the lowry method and freeze\|drying weighing method, there was no significant difference between the three methods. And this established DAS\|ELISA method was simple and could detect concentration of PoIFN\|α in serum.

Key words: porcine α\, interferon, double antibody sandwich ELISA, quantitative determination