关键词: 链霉菌702, 诱导子, 浓度, 筛选

Abstract: Promotion effects on synthesis of antifungalmycin 702 by Streptomyces 702 were conducted by adding abiotic and biotic elicitors. The results showed that promotion effect was obvious by adding threonine and sorbitol into Streptomyces 702 cultures at 32 and 64 h， in which the inhibition zone diameter of adding threonine was 3025% higher than the control. Biological elicitors， Staphylococcus aureus had obvious promotion effect on antifungalmycin 702 synthesis at both 64 and 96 h， especially at 64 h， and Bacillus subtilis had promotion effect at only 64 h. While， Aspergillus niger and Penicillium orange had no effect. Escherichia coli elicitor had promotion effect when adding at 0， 32， 64 and 96 h， and the best effect was achieved at 32 h whose inhibition zone diameter was 3967% higher than the control. The optimum adding concentrations of threonine and E. coli were 50 and 3 μg·mL-1， respectively， and the inhibition zone diameters were 25.78% and 51.17% higher than the control.

Key words: antifungalmycin 702, elicitors, concentration, screen