碳源对多粘类芽孢杆菌生长和多粘菌素E合成的影响

浙江农业学报

碳源对多粘类芽孢杆菌生长和多粘菌素E合成的影响

（1.浙江工业大学生物工程学院，浙江杭州 310014； 2.浙江钱江生物化学股份有限公司，浙江海宁 314400）

出版日期:2016-08-25 发布日期:2016-08-04

Effect of carbon sources on growth of Paenibacillus polymyxa and polymyxin E synthesis

（1. College of Biotechnology and Bioengineering， Zhejiang University of Technology， Hangzhou 310014， China; 2. Zhejiang Qianjiang Biochemical Co.， Ltd， Haining 314400， China）

Online:2016-08-25 Published:2016-08-04

摘要/Abstract

摘要： 为探究提高多粘菌素E产量的方法，在发酵培养基中分别添加葡萄糖、蔗糖、麦芽糖、乳糖和可溶性淀粉，应用平板菌落计数和HPLC法研究了其对多粘类芽孢杆菌生长和多粘菌素E产量的影响。结果表明，5种碳源对多粘类芽孢杆菌的生长和多粘菌素E产量的影响不同，可溶性淀粉是菌体生长的最适碳源，且多粘菌素E产量依次为可溶性淀粉>乳糖>蔗糖>麦芽糖>葡萄糖。可溶性淀粉有助于延长多粘菌素E的产素期和提高其合成速率，与葡萄糖作碳源相比，多粘菌素E的产量和合成速率分别提高了111%和60%。因此，可溶性淀粉是多粘菌素E合成的最适碳源。

关键词: 多粘类芽孢杆菌, 多粘菌素E, 碳源, 可溶性淀粉

Abstract: In order to explore new methods to improve the yield of polymyxin E， five different carbon sources including glucose， sucrose， maltose， lactose and soluble starch were added in the fermentation medium， respectively. The influence on Paenibacillus polymyxa growth and polymyxin E production of different carbon sources were investigated through plate counting method and HPLC method. The results indicated that the effects of different carbon sources on the growth of Paenibacillus polymyxa and the polymyxin E production varied significantly. The effect of these carbon sources on polymyxin E production was: soluble starch>lactose>sucrose>maltose>glucose. Soluble starch promoted both cell growth and polymyxin E yield by extending the polymyxin E biosynthetic period and promoting the synthetic rate. Compared with glucose as carbon source, the yield and the synthetic rate of polymyxin E were increased by 111% and 60%， respectively. Therefore， soluble starch was the optimal candidate for carbon sources among the five carbohydrates in polymyxin E synthese.

Key words: Paenibacillus polymyxa, polymyxin E, carbon source, soluble starch

孙仲奇1，裘娟萍1，陆建卫2，赵春田1，*. 碳源对多粘类芽孢杆菌生长和多粘菌素E合成的影响[J]. 浙江农业学报.

SUN Zhongqi1， QIU Juanping1， LU Jianwei2， ZHAO Chuntian1，*. Effect of carbon sources on growth of Paenibacillus polymyxa and polymyxin E synthesis[J]. .

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