浙江农业学报 ›› 2017, Vol. 29 ›› Issue (11): 1862-1867.DOI: 10.3969/j.issn.1004-1524.2017.11.12

• 植物保护 • 上一篇    下一篇

杯鞘石斛链格孢病菌生物学特性

梁林波1, 王仕玉2, 杨建华1, *, 张雨思1   

  1. 1.云南省林业科学院 漾濞核桃研究院,云南 漾濞 672500;
    2.云南农业大学 园林园艺学院,云南 昆明 650201
  • 收稿日期:2017-05-12 出版日期:2017-11-20 发布日期:2017-12-05
  • 通讯作者: 杨建华,E-mail:414078502@qq.com
  • 作者简介:梁林波(1982—),女,云南宾川人,学士,研究实习员,主要从事林下种植及林产品加工研究工作。E-mail:1297624480@qq.com

Biological characteristics of Alternaria tenuis Nees on Dendrobium gratiosissimum Rchb. f.

LIANG Linbo1, WANG Shiyu2, YANG Jianhua1, *, ZHANG Yusi1   

  1. 1. Yangbi Hickory Research Institute, Yunnan Academy of Forestry, Yangbi 672500, China;
    2. Ficulty of Landscape and Horticulture, Yunnan Agriculture University, Kunming 650201, China
  • Received:2017-05-12 Online:2017-11-20 Published:2017-12-05

摘要: 对杯鞘石斛链格孢病菌的生物学特性进行了研究,结果表明,温度、pH值、光照、碳源和氮源对其生长和产孢有一定的影响。链格孢病菌最适宜生长的温度是25 ℃,在40 ℃几乎不能生长;最适宜的pH值为5~6;25 ℃ 12 h/12 h (L/D)光暗交替培养产孢量最多;能较好地利用蔗糖、葡萄糖、乳糖和淀粉碳源,但对不同的碳源利用有差异;供试氮源中,在含硝酸铵的培养基中菌落生长直径最大,而含甘氨酸的培养基中菌落生长较差。

关键词: 杯鞘石斛, 链格孢菌, pH, 碳源, 氮源, 菌落直径

Abstract: Biological characteristics of Alternaria tenuis Nees on Dendrobium gratiosissimum Rchb. f. were studied. The results showed that temperature, pH, light, carbon source and nitrogen source affected the growth and sporulation of the fungus. The optimal growth temperature was 25 ℃, while Alternaria tenuis Nees almost could not grow at 40 ℃. The optimal pH was 5.0-6.0. The optimal light conditon for sporulation was 12 h lighting and 12 h dark circle in a day at 25 ℃; Sucrose, glucose, lactose and starch could be used as carbon sources at different level in PSA medium. So did the nitrogen sources. Colony diameter was the biggest when NH4NO3 was used as nitrogen source, while smallest when glycine was used as nitrogen source.

Key words: Dendrobium gratiosissimum Rchb. f., Alternaria tenuis Nees, pH, carbon source, nitrogen source, colony diameter

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