关键词: 草莓, FaMYB5, 转录因子, 基因克隆, 表达模式

Abstract: The fulllength cDNA sequence of FaMYB5 gene was cloned from strawberry（Fragaria×ananassa cv. Toyonaka）through homology cloning method. Then， bioinformatics and expression pattern of FaMYB5 were analyzed by some softwares and realtime fluorescent quantitative PCR technique， respectively. Sequence analysis showed that the cDNA of FaMYB5 was 822 bp， encoding 273 amino acids， while the estimated molecular weight and isoelectric point of the putative protein were 29159 ku and 5709， and shared high identity of 957% with FvMYB5 from woodland strawberry. The expression analysis showed that the expression level of FaMYB5 was gradually increasing during fruit development. The lowest expression was detected at small green (SG) stage, and a reduced peak appeared at white stage, when the expression level was merely higher than SG stage. Thereafter, the expression increased gradually from initially red(IR) stage to the full red(FR) stage when the highest level was detected. In short, fulllength cDNA of FaMYB5 was cloned from strawberry, and the expression levels were different during fruit development.

Key words: strawberry, FaMYB5, transcription factor, gene cloning, expression pattern