怀玉山高山马铃薯茎尖再生苗6种病毒的DAS-ELISA检测与分析

doi:10.3969/j.issn.1004-1524.2017.10.15

浙江农业学报 ›› 2017, Vol. 29 ›› Issue (10): 1699-1705.DOI: 10.3969/j.issn.1004-1524.2017.10.15

怀玉山高山马铃薯茎尖再生苗6种病毒的DAS-ELISA检测与分析

尹明华, 刘燕, 郁雪婷, 李远芳, 周榕, 范亚红, 罗玉婕, 万小霞

上饶师范学院生命科学学院,江西上饶 334001

收稿日期:2017-04-01 出版日期:2017-10-20 发布日期:2017-12-05
作者简介:尹明华(1973-),女,江西永新人,硕士,副教授,主要从事植物生物技术方面的研究。E-mail: yinminghua04@163.com
基金资助:
上饶师范学院2017年大学生创新创业训练计划项目(2017-CX-72)

DAS-ELISA detection and analysis of six kinds of viruses in plantlets regenerated from Huaiyushan high mountain potato shoot-tips

YIN Minghua, LIU Yan, YU Xueting, LI Yuanfang, ZHOU Rong, FAN Yahong, LUO Yujie, WAN Xiaoxia

College of Life Sciences, Shangrao Normal University, Shangrao 334001, China

Received:2017-04-01 Online:2017-10-20 Published:2017-12-05

摘要/Abstract

摘要： 对怀玉山高山马铃薯茎尖再生苗6种病毒(马铃薯卷叶病毒、马铃薯Y病毒、马铃薯X病毒、马铃薯S病毒、马铃薯M病毒和马铃薯A病毒)进行了DAS-ELISA检测与分析,并比较不同茎尖脱毒方法的效率,旨在确定怀玉山高山马铃薯的病毒种类,并筛选出怀玉山高山马铃薯脱毒苗。结果表明,怀玉山高山马铃薯感染的病毒包括马铃薯卷叶病毒、马铃薯Y病毒、马铃薯X病毒、马铃薯S病毒、马铃薯M病毒和马铃薯A病毒6种。“单纯的茎尖培养”以及“茎尖培养→热处理→茎尖培养”只能脱除部分病毒,而“茎尖培养→热处理→茎尖培养→热处理→茎尖培养”可脱除全部6种病毒。本试验成功获得了脱毒效果较好的编号为5-1-2和6-4-1以及脱毒效果最好的编号为5-3-2和6-2-2的怀玉山高山马铃薯茎尖再生脱毒苗。本试验结果为怀玉山高山马铃薯脱毒苗的工业化生产及其主粮化背景下高山马铃薯的产业化发展提供了技术支撑和理论依据。

关键词: 怀玉山高山马铃薯, 茎尖再生苗, 病毒, 双抗体夹心酶联免疫吸附测定法

Abstract: In order to determine the virus species and screen out its virus-free plantlets, 6 viruses (potato leafroll virus, potato virus Y, potato virus X, potato virus S, potato virus M and potato virus A) in plantlets regenerated from shoot-tips of Huaiyushan high mountain potato were detected and analyzed by DAS-ELISA, and the efficiency of different shoot tip detoxification methods were compared. The results showed that there were 6 viruses including potato leafroll virus, potato virus Y, potato virus X, potato virus S, potato virus M and potato virus A in Huaiyushan high mountain potato. Two methods such as “simple shoot tip culture” and “shoot tip culture→thermotherapy→shoot tip culture” can only remove parts of the viruses, while “shoot tip culture→thermotherapy→shoot tip culture→thermotherapy→shoot tip culture” could remove all 6 viruses. In this study, we successfully obtained the virus-free plantlets of Huaiyushan high mountain potato, of which No. 5-1-2 and No. 6-4-1 had better virus-free effect and No. 5-3-2 and No. 6-2-2 had the best virus-free effect. The results will help to provide technical support and theoretical basis for virus-free plantlet industrial production of Huaiyushan high mountain potato and its industrialization development under the background of the fourth major staple food.

Key words: Huaiyushan high mountain potato, plantlets regenerated from shoot-tips, virus, DAS-ELISA

中图分类号:

S532

尹明华, 刘燕, 郁雪婷, 李远芳, 周榕, 范亚红, 罗玉婕, 万小霞. 怀玉山高山马铃薯茎尖再生苗6种病毒的DAS-ELISA检测与分析[J]. 浙江农业学报, 2017, 29(10): 1699-1705.

YIN Minghua, LIU Yan, YU Xueting, LI Yuanfang, ZHOU Rong, FAN Yahong, LUO Yujie, WAN Xiaoxia. DAS-ELISA detection and analysis of six kinds of viruses in plantlets regenerated from Huaiyushan high mountain potato shoot-tips[J]. , 2017, 29(10): 1699-1705.

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怀玉山高山马铃薯茎尖再生苗6种病毒的DAS-ELISA检测与分析

DAS-ELISA detection and analysis of six kinds of viruses in plantlets regenerated from Huaiyushan high mountain potato shoot-tips

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