氨氮降解微生物的筛选和初步应用

doi:10.3969/j.issn.1004-1524.2020.09.17

浙江农业学报 ›› 2020, Vol. 32 ›› Issue (9): 1683-1691.DOI: 10.3969/j.issn.1004-1524.2020.09.17

氨氮降解微生物的筛选和初步应用

孙筱君¹, 沈琦², 吴逸飞², 姚晓红², 李园成², 孙宏², 王新², 汤江武², 葛向阳^1,*

1.华中农业大学生命科学与技术学院,湖北武汉 430070;
2.浙江省农业科学院植物保护与微生物研究所,浙江杭州 310021

收稿日期:2020-03-24 出版日期:2020-09-25 发布日期:2020-10-10
通讯作者: *葛向阳,E-mail:gxy@mail.hzau.edu.cn
作者简介:孙筱君(1994—),女,湖北荆州人,硕士研究生,主要从事养殖粪污微生物处理研究。E-mail:531911993@qq.com
基金资助:
国家自然科学基金青年科学基金(41907213);浙江省重点研发计划(2018C02044-4)

Screening and utilization of ammonia-nitrogen-degrading microorganism

SUN Xiaojun¹, SHEN Qi², WU Yifei², YAO Xiaohong², LI Yuancheng², SUN Hong², WANG Xin², TANG Jiangwu², GE Xiangyang^1,*

1. College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, China;
2. Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China

Received:2020-03-24 Online:2020-09-25 Published:2020-10-10

摘要/Abstract

摘要： 为了创制高效的粪污氨氮降解菌剂,特从猪粪中分离高效的氨氮降解微生物。通过感官法初筛,并结合氨氮降解率为指标进行复筛,最终获得高效氨氮降解菌株——Z2、AOZ和Y。经形态学观察、生理生化鉴定、16S rRNA基因序列分析,确定菌株Z2为弯曲芽孢杆菌(Bacillus flexus),菌株AOZ为恶臭假单胞菌(Pseudomonas putida),Y为干酪乳杆菌(Lactobacillus casei)。试验结果显示,菌株Z2和Y组成的复配菌剂对氨氮的降解效果最优,氨氮降解率高达53.19%,表明该复合菌剂可以应用于粪污除氨。

关键词: 猪粪, 除氨微生物, 筛选, 复配

Abstract: To obtain the ammonia-lowering agent for livestock manure, the bacterial strains with good ammonia-nitrogen-degrading capability were isolated from swine manure. Three strains with high ammonia-nitrogen degradation efficiency were identified with the olfactory threshold method combined with the comparisons of their ammonia-nitrogen degradation efficiencies, and were encoded as Z2, AOZ and Y, respectively. Based on the results of morphological observation, physiological and biochemical tests, and 16S rRNA sequence homology analysis, the strains Z2, AOZ and Y were identified as Bacillus flexus, Pseudomonas putida, and Lactobacillus casei, respectively. The experimental results showed that the ammonia-nitrogen degradation efficiency was higher when strains Z2 and Y were mixed, and the highest ammonia-nitrogen degradation efficiency reached 53.19%, suggesting that the microorganism complex could be practically applied to remove ammonium from swine manure.

Key words: pig manure, ammonia-nitrogen-degrading microorganism, screening, compounding

中图分类号:

S182
X172

孙筱君, 沈琦, 吴逸飞, 姚晓红, 李园成, 孙宏, 王新, 汤江武, 葛向阳. 氨氮降解微生物的筛选和初步应用[J]. 浙江农业学报, 2020, 32(9): 1683-1691.

SUN Xiaojun, SHEN Qi, WU Yifei, YAO Xiaohong, LI Yuancheng, SUN Hong, WANG Xin, TANG Jiangwu, GE Xiangyang. Screening and utilization of ammonia-nitrogen-degrading microorganism[J]. , 2020, 32(9): 1683-1691.

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氨氮降解微生物的筛选和初步应用

Screening and utilization of ammonia-nitrogen-degrading microorganism

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