金华猪肌肉和血清氨基酸谱的发育性变化及其与肌肉生长的相关性

doi:10.3969/j.issn.1004-1524.2022.04.04

浙江农业学报 ›› 2022, Vol. 34 ›› Issue (4): 687-694.DOI: 10.3969/j.issn.1004-1524.2022.04.04

金华猪肌肉和血清氨基酸谱的发育性变化及其与肌肉生长的相关性

李景上¹^,²(), 章啸君³, 陈胜昌¹, 蒋锦华⁴, 项云³, 屠平光³, 楼芳芳³, 杨华², 肖英平²^,^*()

1.贵州大学动物科学学院,贵州贵阳 550025
2.浙江农业科学院农产品质量安全与营养研究所,省部共建农产品质量安全危害因子与风险防控国家重点实验室,浙江杭州 310021
3.金华市农业科学研究院畜牧兽医研究所,浙江金华 321017
4.金华市金东区动物防疫检疫站,浙江金华 321015

收稿日期:2021-04-12 出版日期:2022-04-25 发布日期:2022-04-28
通讯作者: 肖英平
作者简介:*肖英平,E-mail: ypxiaozju@126.com
李景上(1995—),男,贵州六盘水人,硕士研究生,研究方向为动物营养与饲料科学。E-mail: jingshangligzu@126.com
基金资助:
国家自然科学基金(31972999)

Developmental changes of amino acid profiles in muscle and serum and their correlation with muscle growth of Jinhua pigs

LI Jingshang¹^,²(), ZHANG Xiaojun³, CHEN Shengchang¹, JIANG Jinhua⁴, XIANG Yun³, TU Pingguang³, LOU Fangfang³, YANG Hua², XIAO Yingping²^,^*()

1. College of Animal Science, Guizhou University, Guiyang 550025, China
2. State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Institute of Agro-Product Safety and Nutrition, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China
3. Institute of Animal Husbandry and Veterinary Medicine, Jinhua Academy of Agricultural Sciences, Jinhua 321017, Zhejiang, China
4. Animal Quarantine Station of Jindong District, Jinhua City, Jinhua 321015, Zhejiang, China

Received:2021-04-12 Online:2022-04-25 Published:2022-04-28
Contact: XIAO Yingping

摘要/Abstract

摘要：

选择同批次的60头金华猪仔猪进行饲养,在45、90、150、270日龄分别选择4头金华猪进行屠宰,测定眼肌面积,并采集背最长肌和血清样品,分别用于水解氨基酸和游离氨基酸及其衍生物的测定。试验结果显示:随着日龄增加,金华猪眼肌面积显著(P<0.05)增大。肌肉水解氨基酸中:必需氨基酸含量和总氨基酸含量在45日龄至270日龄间先显著(P<0.05)升高,再显著(P<0.05)下降,之后基本保持稳定;非必需氨基酸含量先显著(P<0.05)升高,再显著(P<0.05)下降,后又显著(P<0.05)升高。血清游离氨基酸中:必需氨基酸含量在45日龄至270日龄间先保持稳定,最后显著(P<0.05)升高;非必需氨基酸含量先显著(P<0.05)降低,再显著(P<0.05)升高,后保持稳定;总氨基酸含量先显著(P<0.05)下降,之后显著(P<0.05)升高。肌肉和血清中的大部分氨基酸含量均与眼肌面积呈正相关,其中,精氨酸、缬氨酸、异亮氨酸和亮氨酸含量与眼肌面积呈较强的正相关,但甘氨酸含量与眼肌面积呈负相关。综上,金华猪肌肉和血清中的氨基酸谱呈现发育性动态变化,精氨酸、缬氨酸、异亮氨酸和亮氨酸含量变化与肌肉生长存在较强相关性。

关键词: 金华猪, 氨基酸谱, 眼肌面积, 肌肉生长, 发育性变化

Abstract:

In the present study, a total of 60 Jinhua piglets from the same batch were fed with commercial diets under the standard management. Four Jinhua pigs were randomly selected and slaughtered at 45, 90, 150 and 270 days of age, respectively, to determine the ribeye areas, and longissimus dorsi muscle and serum samples were collected to determine the content of amino acids and their derivatives. The results showed that the ribeye areas of Jinhua pigs significantly (P<0.05) increased with age. In the longissimus dorsi: essential amino acids content and total amino acids content significantly (P<0.05) increased first, then significantly (P<0.05) decreased and remained stable after; while nonessential amino acids content increased significantly (P<0.05) first, decreased significantly (P<0.05) later and then increased significantly (P<0.05). In the serum: essential amino acids content remained stable first, and then increased significantly (P<0.05); nonessential amino acids content decreased significantly (P<0.05) first, increased significantly (P<0.05) later and then remained stable; total amino acid content decreased significantly (P<0.05) first, then increased significantly (P<0.05). Most of the amino acids contents in muscle and serum were positively correlated with ribeye areas, where the contents of arginine, valine, isoleucine and leucine contents gave a strong positive correlation with ribeye areas, but glycine content in the muscle and serum was negatively associated with ribeye areas. In summary, the amino acid profiles in the muscle and serum of Jinhua pigs presented developmental changes, and arginine, valine, isoleucine and leucine contents were positively correlated with muscle growth.

Key words: Jinhua pigs, amino acid profile, ribeye areas, muscle growth, developmental changes

中图分类号:

S852.21

李景上, 章啸君, 陈胜昌, 蒋锦华, 项云, 屠平光, 楼芳芳, 杨华, 肖英平. 金华猪肌肉和血清氨基酸谱的发育性变化及其与肌肉生长的相关性[J]. 浙江农业学报, 2022, 34(4): 687-694.

LI Jingshang, ZHANG Xiaojun, CHEN Shengchang, JIANG Jinhua, XIANG Yun, TU Pingguang, LOU Fangfang, YANG Hua, XIAO Yingping. Developmental changes of amino acid profiles in muscle and serum and their correlation with muscle growth of Jinhua pigs[J]. Acta Agriculturae Zhejiangensis, 2022, 34(4): 687-694.

图/表 7

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项目Item	D45	D90	D150	D270
组氨酸Histidine^*	0.91±0.06 ab	1.14±0.02 a	0.62±0.08 c	0.88±0.12 b
苏氨酸Threonine^*	1.09±0.03	1.14±0.01	1.07±0.03	1.10±0.02
精氨酸Arginine^*	1.36±0.06 bc	1.53±0.12 ab	1.16±0.05 c	1.70±0.13 a
缬氨酸Valine^*	1.13±0.06 c	1.40±0.03 ab	1.29±0.05 b	1.50±0.05 a
异亮氨酸Isoleucine^*	1.05±0.06 b	1.28±0.02 a	1.16±0.06 ab	1.17±0.02 ab
亮氨酸Leucine^*	1.81±0.09 b	2.17±0.04 a	2.01±0.12 ab	2.21±0.05 a
赖氨酸Lysine^*	2.06±0.10 b	2.35±0.05 a b	2.16±0.17 b	2.58±0.08 a
甲硫氨酸Methionine^*	1.59±0.12 b	2.87±0.12 a	3.14±0.03 a	0.56±0.02 c
苯丙氨酸Phenylalanine^*	0.97±0.04 c	1.15±0.02 ab	1.00±0.05 b	1.23±0.08 a
天冬氨酸Aspartic acid	2.10±0.10 b	2.53±0.05 a	2.21±0.11 b	2.52±0.07 a
谷氨酸Glutamic acid	3.62±0.18 b	4.34±0.09 a	3.84±0.22 ab	4.23±0.12 a
丝氨酸Serine	0.90±0.04 b	1.07±0.02 a	0.94±0.05 b	1.06±0.02 a
甘氨酸Glycine	0.97±0.05 b	1.16±0.02 a	1.11±0.09 ab	0.99±0.02 ab
丙氨酸Alanine	1.34±0.05 c	1.54±0.03 a	1.40±0.07 bc	1.49±0.03 ab
酪氨酸Tyrosine	0.55±0.04 b	0.73±0.03 a	0.57±0.02 b	0.68±0.02 a
脯氨酸Proline	0.89±0.04	0.97±0.02	0.83±0.05	1.17±0.25
半胱氨酸Cysteine	0.05±0.01	0.05±0.01	0.04±0.01	0.05±0.01
必需氨基酸Essential amino acids	11.95±0.46 c	15.02±0.33 a	13.60±0.46 b	12.92±0.09 bc
非必需氨基酸Nonessential amino acids	10.41±0.50 b	12.37±0.26 a	10.94±0.54 b	12.17±0.06 a
总氨基酸Total amino acids	22.36±0.93 c	27.39±0.59 a	24.53±0.97 bc	25.08±0.10 b

项目Item	D45	D90	D150	D270
组氨酸Histidine^*	0.91±0.06 ab	1.14±0.02 a	0.62±0.08 c	0.88±0.12 b
苏氨酸Threonine^*	1.09±0.03	1.14±0.01	1.07±0.03	1.10±0.02
精氨酸Arginine^*	1.36±0.06 bc	1.53±0.12 ab	1.16±0.05 c	1.70±0.13 a
缬氨酸Valine^*	1.13±0.06 c	1.40±0.03 ab	1.29±0.05 b	1.50±0.05 a
异亮氨酸Isoleucine^*	1.05±0.06 b	1.28±0.02 a	1.16±0.06 ab	1.17±0.02 ab
亮氨酸Leucine^*	1.81±0.09 b	2.17±0.04 a	2.01±0.12 ab	2.21±0.05 a
赖氨酸Lysine^*	2.06±0.10 b	2.35±0.05 a b	2.16±0.17 b	2.58±0.08 a
甲硫氨酸Methionine^*	1.59±0.12 b	2.87±0.12 a	3.14±0.03 a	0.56±0.02 c
苯丙氨酸Phenylalanine^*	0.97±0.04 c	1.15±0.02 ab	1.00±0.05 b	1.23±0.08 a
天冬氨酸Aspartic acid	2.10±0.10 b	2.53±0.05 a	2.21±0.11 b	2.52±0.07 a
谷氨酸Glutamic acid	3.62±0.18 b	4.34±0.09 a	3.84±0.22 ab	4.23±0.12 a
丝氨酸Serine	0.90±0.04 b	1.07±0.02 a	0.94±0.05 b	1.06±0.02 a
甘氨酸Glycine	0.97±0.05 b	1.16±0.02 a	1.11±0.09 ab	0.99±0.02 ab
丙氨酸Alanine	1.34±0.05 c	1.54±0.03 a	1.40±0.07 bc	1.49±0.03 ab
酪氨酸Tyrosine	0.55±0.04 b	0.73±0.03 a	0.57±0.02 b	0.68±0.02 a
脯氨酸Proline	0.89±0.04	0.97±0.02	0.83±0.05	1.17±0.25
半胱氨酸Cysteine	0.05±0.01	0.05±0.01	0.04±0.01	0.05±0.01
必需氨基酸Essential amino acids	11.95±0.46 c	15.02±0.33 a	13.60±0.46 b	12.92±0.09 bc
非必需氨基酸Nonessential amino acids	10.41±0.50 b	12.37±0.26 a	10.94±0.54 b	12.17±0.06 a
总氨基酸Total amino acids	22.36±0.93 c	27.39±0.59 a	24.53±0.97 bc	25.08±0.10 b

项目Item	D45	D90	D150	D270
组氨酸Histidine^*	14.32±1.04 ab	14.02±1.19 b	16.18±1.03 ab	17.48±0.65 a
苏氨酸Threonine^*	18.35±1.77 b	19.57±0.86 b	22.00±2.11 ab	26.00±1.27 a
精氨酸Arginine^*	24.64±2.37 b	30.48±0.82 ab	32.95±3.41 a	37.93±2.65 a
缬氨酸Valine^*	29.58±3.16 bc	25.69±1.21 c	35.96±1.98 b	47.63±2.41 a
异亮氨酸Isoleucine^*	15.66±0.57 b	14.76±1.57 b	18.61±2.02 ab	22.40±1.34 a
亮氨酸Leucine^*	28.02±1.57 bc	26.07±1.35 c	30.99±0.67 b	37.29±1.91 a
赖氨酸Lysine^*	33.26±2.86 ab	25.57±2.65 b	27.03±3.66 b	35.92±0.94 a
甲硫氨酸Methionine^*	7.13±0.28 b	7.94±0.58 ab	8.15±0.39 ab	8.70±0.29 a
苯丙氨酸Phenylalanine^*	19.12±4.03	15.42±1.24	18.52±1.28	20.86±0.98
天冬氨酸Aspartic acid	8.65±1.05 ab	6.08±0.26 b	5.79±0.35 b	11.45±1.82 a
谷氨酸Glutamic acid	86.61±4.26 b	59.91±5.35 c	70.44±2.09 bc	112.08±8.82 a
丝氨酸Serine	40.84±4.08 a	22.41±2.41 b	21.14±1.55 b	21.31±0.82 b
甘氨酸Glycine	68.25±1.94 a	42.50±3.53 b	65.37±5.46 a	62.01±3.09 a
丙氨酸Alanine	51.79±3.91	52.03±4.62	58.84±3.81	61.86±5.85
酪氨酸Tyrosine	14.68±0.75 b	16.21±0.97 b	18.47±0.24 a	18.23±0.33 a
脯氨酸Proline	34.20±3.01 ab	36.65±1.36 a	39.44±3.87 a	26.86±1.66 b
半胱氨酸Cysteine	0.02±0.02	0.07±0.06	0.35±0.13	0.71±0.41
必需氨基酸Essential amino acids	190.07±11.02 b	179.52±3.09 b	210.60±14.19 b	254.20±8.13 a
非必需氨基酸	305.03±5.20 a	235.85±14.09 b	279.84±4.67 a	314.50±18.06 a
Nonessential amino acids
总氨基酸Total amino acids	495.10±11.79 b	415.36±16.17 c	490.44±9.56 b	568.70±26.11 a

项目Item	D45	D90	D150	D270
组氨酸Histidine^*	14.32±1.04 ab	14.02±1.19 b	16.18±1.03 ab	17.48±0.65 a
苏氨酸Threonine^*	18.35±1.77 b	19.57±0.86 b	22.00±2.11 ab	26.00±1.27 a
精氨酸Arginine^*	24.64±2.37 b	30.48±0.82 ab	32.95±3.41 a	37.93±2.65 a
缬氨酸Valine^*	29.58±3.16 bc	25.69±1.21 c	35.96±1.98 b	47.63±2.41 a
异亮氨酸Isoleucine^*	15.66±0.57 b	14.76±1.57 b	18.61±2.02 ab	22.40±1.34 a
亮氨酸Leucine^*	28.02±1.57 bc	26.07±1.35 c	30.99±0.67 b	37.29±1.91 a
赖氨酸Lysine^*	33.26±2.86 ab	25.57±2.65 b	27.03±3.66 b	35.92±0.94 a
甲硫氨酸Methionine^*	7.13±0.28 b	7.94±0.58 ab	8.15±0.39 ab	8.70±0.29 a
苯丙氨酸Phenylalanine^*	19.12±4.03	15.42±1.24	18.52±1.28	20.86±0.98
天冬氨酸Aspartic acid	8.65±1.05 ab	6.08±0.26 b	5.79±0.35 b	11.45±1.82 a
谷氨酸Glutamic acid	86.61±4.26 b	59.91±5.35 c	70.44±2.09 bc	112.08±8.82 a
丝氨酸Serine	40.84±4.08 a	22.41±2.41 b	21.14±1.55 b	21.31±0.82 b
甘氨酸Glycine	68.25±1.94 a	42.50±3.53 b	65.37±5.46 a	62.01±3.09 a
丙氨酸Alanine	51.79±3.91	52.03±4.62	58.84±3.81	61.86±5.85
酪氨酸Tyrosine	14.68±0.75 b	16.21±0.97 b	18.47±0.24 a	18.23±0.33 a
脯氨酸Proline	34.20±3.01 ab	36.65±1.36 a	39.44±3.87 a	26.86±1.66 b
半胱氨酸Cysteine	0.02±0.02	0.07±0.06	0.35±0.13	0.71±0.41
必需氨基酸Essential amino acids	190.07±11.02 b	179.52±3.09 b	210.60±14.19 b	254.20±8.13 a
非必需氨基酸	305.03±5.20 a	235.85±14.09 b	279.84±4.67 a	314.50±18.06 a
Nonessential amino acids
总氨基酸Total amino acids	495.10±11.79 b	415.36±16.17 c	490.44±9.56 b	568.70±26.11 a

项目Item	D45	D90	D150	D270
γ-氨基正丁酸γ-aminobutyric acid	0.09±0.01 a	0.08±0.01 a	0.10±0.01 a	0.01±0.01 b
牛磺酸Taurine	31.76±2.04 b	25.81±2.34 b	29.75±2.00 b	42.92±3.40 a
α-氨基己二酸α-aminoadipic acid	12.54±2.59 a	9.12±1.04 ab	7.17±0.53 b	9.46±1.49 ab
瓜氨酸Citrulline	9.12±0.65 b	9.82±0.63 b	13.80±1.21 a	11.40±1.46 ab
鸟氨酸Ornithine	16.08±1.24 a	10.80±1.60 b	13.12±0.94 ab	12.59±0.96 ab
胱硫醚Cystathionine	2.03±0.18	1.99±0.32	1.84±0.19	2.18±0.08
α-氨基正丁酸α-aminobutyric acid	1.71±0.34	1.68±0.22	1.69±0.17	3.17±0.85
β-丙氨酸β-alanine	1.70±0.23 a	1.68±0.07 a	1.07±0.03 b	0.89±0.15 b
β-氨基异丁酸β-aminoisobutyric acid	0.01±0.01	0.01±0.01	0.01±0.01	0.32±0.26
肌肽Carnosine	4.62±0.48	4.41±0.29	5.02±0.49	4.17±0.62
乙醇胺Ethanolamine	2.28±0.10 ab	2.10±0.19 b	2.08±0.21 b	2.71±0.09 a
羟脯氨酸Hydroxyproline	10.32±1.15 a	6.94±0.49 b	6.78±0.35 b	3.77±0.81 c
1-甲基组氨酸1-methylhistidine	2.45±0.30 a	1.66±0.18 b	1.16±0.09 b	0.39±0.24 c
3-甲基组氨酸3-methylhistidine	1.48±0.12 ab	1.37±0.16 ab	1.20±0.09 b	1.66±0.16 a
磷酸丝氨酸Phosphoserine	2.01±0.12 b	1.50±0.14 c	1.55±0.05 c	3.16±0.23 a
尿素Urea	103.33±17.57 c	190.10±10.18 b	239.03±5.50 ab	249.41±25.86 a

金华猪肌肉和血清氨基酸谱的发育性变化及其与肌肉生长的相关性

Developmental changes of amino acid profiles in muscle and serum and their correlation with muscle growth of Jinhua pigs

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