基于广泛靶向代谢组学的武穴野生山药及其零余子代谢物差异分析

doi:10.3969/j.issn.1004-1524.2022.04.09

浙江农业学报 ›› 2022, Vol. 34 ›› Issue (4): 727-735.DOI: 10.3969/j.issn.1004-1524.2022.04.09

基于广泛靶向代谢组学的武穴野生山药及其零余子代谢物差异分析

靳晓杰¹(), 张静珍¹^,², 杨新笋¹^,^*()

1.湖北省农业科学院粮食作物研究所,湖北武汉 430064
2.长江大学农学院,湖北荆州 434025

收稿日期:2021-08-05 出版日期:2022-04-25 发布日期:2022-04-28
通讯作者: 杨新笋
作者简介:*杨新笋,E-mail: yangxins013@163.com
靳晓杰(1986—),女,河南周口人,博士,助理研究员,主要从事薯芋类遗传育种研究。E-mail: xiaojiejin26@163.com
基金资助:
湖北省农业科技创新中心资助项目(2019-620-001-03);湖北省重点研发计划(2020BHB024)

Comparative metabolite profiling of rhizome and bulbil of wild Chinese yam (Dioscorea polystachya Turcz.) from Wuxue by widely targeted metabolomics

JIN Xiaojie¹(), ZHANG Jingzhen¹^,², YANG Xinsun¹^,^*()

1. Food Crops Institute, Hubei Academy of Agricultural Sciences, Wuhan 430064, China
2. College of Agriculture, Yangtze University, Jingzhou 434025,Hubei,China

Received:2021-08-05 Online:2022-04-25 Published:2022-04-28
Contact: YANG Xinsun

摘要/Abstract

摘要：

为揭示武穴野生山药及其零余子中代谢物的种类与相对含量差异,利用LC-MS/MS广泛靶向代谢组学的方法对采集自湖北省武穴市野生山药的山药肉、山药皮和零余子中代谢物成分进行检测,并结合多元统计分析和单变量统计分析筛选零余子与山药肉间、山药皮与山药肉间的差异代谢物。结果表明:武穴野生山药及其零余子中共检测到385种代谢物;在零余子与山药肉间共筛选到70种差异代谢物,其中,43种代谢物在零余子中相对含量较高,27种代谢物在山药肉中相对含量较高。在山药皮与山药肉间共筛选出81种差异代谢物,其中,46种代谢物在山药皮中相对含量较高,35种代谢物在山药肉中相对含量较高。武穴野生山药的山药肉中富含缬氨酸、甲硫氨酸、丙氨酸等多种氨基酸,零余子中富含异柚葡糖苷、C-hexosyl-chrysoeriol 5-O-hexoside、扁蓄苷等18种黄酮类代谢物,山药皮中则富含反式玉米素-O-葡糖苷核苷、乙酰左旋肉碱、维生素B₂、C-hexosyl-chrysoeriol 7-O-hexoside、槲皮素-3-甲基醚等代谢物。综上,武穴野生山药的块茎及其零余子中含有丰富的代谢物,可根据代谢物种类、功能与用途合理开发应用。

关键词: 山药, 零余子, 广泛靶向代谢组, 差异代谢物

Abstract:

To clarify the metabolites and their relative contents in rhizome and bulbil of wild Chinese Yam (Dioscorea polystachya Turcz.) from Wuxue, widely targeted LC-MS/MS was used to determine the metabolite profiles in rhizome flesh, rhizome peel, and bulbil of wild Chinese yam from Wuxue, Hubei Province. Differentially accumulated metabolites between bulbil and rhizome flesh and between rhizome peel and rhizome flesh were identified by multivariate and univariate analyses. The result showed that a total of 385 metabolites were detected in rhizome and bulbil of wild Chinese yam from Wuxue. There were 70 differentially accumulated metabolites between bulbil and rhizome flesh, among which 43 metabolites were more significantly accumulated in bulbil, while 27 metabolites had higher accumulation in rhizome flesh. Moreover, a total of 81 differentially accumulated metabolites were identified between rhizome peel and rhizome flesh, including 46 metabolites with higher accumulation in rhizome peel and 35 metabolites with higher accumulation in rhizome flesh. In addition, the rhizome flesh of wild Chinese yam was enriched in amino acids such as valine, methionine and alanine. Compared with rhizome flesh, bulbil had higher levels of 18 flavonoid metabolites such as isohemiphloin, C-hexosyl-chrysoeriol 5-O-hexoside and avicularin, while the rhizome peel was more enriched in trans-zeatin-O-glucoside riboside, acetyl-L-carnitine, vitamin B₂, C-hexosyl-chrysoeriol 7-O-hexoside and quercetin-3-methyl ether. This study demonstrated that rhizome and bulbil of wild Chinese yam from Wuxue contain abundant metabolites, and could be rationally developed and utilized based on the type and function of metabolites.

Key words: Chinese yam, bulbil, widely targeted metabolomics, differentially accumulated metabolites

中图分类号:

S632.1

靳晓杰, 张静珍, 杨新笋. 基于广泛靶向代谢组学的武穴野生山药及其零余子代谢物差异分析[J]. 浙江农业学报, 2022, 34(4): 727-735.

JIN Xiaojie, ZHANG Jingzhen, YANG Xinsun. Comparative metabolite profiling of rhizome and bulbil of wild Chinese yam (Dioscorea polystachya Turcz.) from Wuxue by widely targeted metabolomics[J]. Acta Agriculturae Zhejiangensis, 2022, 34(4): 727-735.

图/表 7

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分类 Class	代谢物数量 Number of Compounds
氨基酸及其衍生物Amino acids and their derivatives	54
黄酮类Flavonoids	54
脂质Lipid	17
核苷酸及其衍生物Nucleotides and their derivatives	28
有机酸Organic acids	44
酚胺Phenolamides	27
植物激素及其衍生物	32
Plant hormones and their derivatives
多酚Polyphenols	14
糖类Sugars	14
维生素及其衍生物Vitamins and their derivatives	8
其他Other	93
总计Total	385

分类 Class	代谢物数量 Number of Compounds
氨基酸及其衍生物Amino acids and their derivatives	54
黄酮类Flavonoids	54
脂质Lipid	17
核苷酸及其衍生物Nucleotides and their derivatives	28
有机酸Organic acids	44
酚胺Phenolamides	27
植物激素及其衍生物	32
Plant hormones and their derivatives
多酚Polyphenols	14
糖类Sugars	14
维生素及其衍生物Vitamins and their derivatives	8
其他Other	93
总计Total	385

代谢物Metabolites	VIP	log₂FC	FDR	UP/DOWN
异柚葡糖苷Isohemiphloin	2.10	5.06	0.009	UP
漆树酸Anacardic acid	2.10	5.41	0.013	UP
C-hexosyl-chrysoeriol 5-O-hexoside	2.09	5.33	0.038	UP
扁蓄苷Avicularin	2.09	5.20	0.035	UP
C-hexosyl-luteolin O-hexoside	2.01	4.50	0.028	UP
2'-磷酸腺苷Adenosine2’-phosphate	2.00	4.65	0.016	UP
金圣草素-6-C戊苷-8-C-己糖苷Chrysoeriol-6-C-pentoside-8-C-hexoside	1.98	4.45	0.001	UP
异鼠李素Isorhamnetin	1.95	4.32	0.009	UP
溶血磷脂酰甘油(16:0)LysoPG 16:0	1.90	4.43	0.029	UP
山奈酚3-半乳糖苷-7-鼠李糖苷Kaempferol 3-galactoside-7-rhamnoside	1.89	4.06	0.018	UP
哌啶酸Pipecolic acid	1.89	3.91	0.019	UP
芹菜素-5-O-葡糖苷Apigenin 5-O-glucoside	1.89	4.50	0.032	UP
异牡荆苷Apigenin 6-C-glucoside	1.88	3.91	0.024	UP
乙酰左旋肉碱Acetyl-L-carnitine	1.86	3.85	0.026	UP
飞燕草素-3-O-芸香糖苷Delphinidin 3-O-rutinoside	1.85	3.76	0.016	UP
乙酰苯胺Acetanilide	1.81	3.57	0.009	UP
维生素B₂Vitamin B₂	1.79	3.93	0.049	UP
缬氨酸Valine	1.76	-5.52	0.009	DOWN
N-甲基苯甲酰胺N-methylbenzamide	1.76	3.30	0.009	UP
甲硫氨酸Methionine	1.76	-5.54	0.013	DOWN

代谢物Metabolites	VIP	log₂FC	FDR	UP/DOWN
异柚葡糖苷Isohemiphloin	2.10	5.06	0.009	UP
漆树酸Anacardic acid	2.10	5.41	0.013	UP
C-hexosyl-chrysoeriol 5-O-hexoside	2.09	5.33	0.038	UP
扁蓄苷Avicularin	2.09	5.20	0.035	UP
C-hexosyl-luteolin O-hexoside	2.01	4.50	0.028	UP
2'-磷酸腺苷Adenosine2’-phosphate	2.00	4.65	0.016	UP
金圣草素-6-C戊苷-8-C-己糖苷Chrysoeriol-6-C-pentoside-8-C-hexoside	1.98	4.45	0.001	UP
异鼠李素Isorhamnetin	1.95	4.32	0.009	UP
溶血磷脂酰甘油(16:0)LysoPG 16:0	1.90	4.43	0.029	UP
山奈酚3-半乳糖苷-7-鼠李糖苷Kaempferol 3-galactoside-7-rhamnoside	1.89	4.06	0.018	UP
哌啶酸Pipecolic acid	1.89	3.91	0.019	UP
芹菜素-5-O-葡糖苷Apigenin 5-O-glucoside	1.89	4.50	0.032	UP
异牡荆苷Apigenin 6-C-glucoside	1.88	3.91	0.024	UP
乙酰左旋肉碱Acetyl-L-carnitine	1.86	3.85	0.026	UP
飞燕草素-3-O-芸香糖苷Delphinidin 3-O-rutinoside	1.85	3.76	0.016	UP
乙酰苯胺Acetanilide	1.81	3.57	0.009	UP
维生素B₂Vitamin B₂	1.79	3.93	0.049	UP
缬氨酸Valine	1.76	-5.52	0.009	DOWN
N-甲基苯甲酰胺N-methylbenzamide	1.76	3.30	0.009	UP
甲硫氨酸Methionine	1.76	-5.54	0.013	DOWN

代谢物Metabolites	VIP	log₂FC	FDR	UP/DOWN
反式玉米素-O-葡糖苷核苷Trans-zeatin-O-glucoside riboside	2.14	6.54	0.012	UP
乙酰左旋肉碱Acetyl-L-carnitine	2.13	5.81	0.009	UP
维生素B₂Vitamin B₂	2.13	6.16	0.016	UP
C-hexosyl-chrysoeriol 7-O-hexoside	2.01	4.88	0.014	UP
槲皮素-3-甲基醚Quercetin 3-methyl ether	1.98	5.67	0.021	UP
乙酰柠檬酸三丁酯Acetyl tributyl citrate	1.96	4.86	0.004	UP
乙酰苯胺Acetanilide	1.94	4.75	0.006	UP
N-甲基苯甲酰胺N-methylbenzamide	1.89	4.46	0.006	UP
MG 18:1	1.84	4.10	0.036	UP
精氨酸Arginine	1.82	-5.92	0.004	DOWN
芹菜素-7-O-葡萄糖醛酸甲酯苷Apigenin 7-O-methylglucuronide	1.76	5.12	0.037	UP
糠酸2-furoate acid	1.75	-5.67	0.045	DOWN
反式玉米素核苷Trans zeatin-riboside	1.73	3.61	0.007	UP
戊烯二酸Glutaconic acid	1.72	-5.53	0.009	DOWN
溶血磷脂酰乙醇胺(16:0)LysoPE 16:0	1.71	3.44	0.006	UP
3-吲哚丁酸Indole-3-butyric acid	1.68	3.25	0.033	UP
D-Arabinonate	1.67	3.26	0.012	UP
十二甲基环六硅氧烷Dodecamethylcyclohexasiloxane	1.67	3.18	0.011	UP
3-[乙基(亚硝基)氨基]丙酸3-[Ethyl(nitroso)amino]propanoic acid	1.65	-5.30	0.012	DOWN
咪唑乙酸Imidazoleacetic acid	1.65	3.18	0.006	UP

基于广泛靶向代谢组学的武穴野生山药及其零余子代谢物差异分析

Comparative metabolite profiling of rhizome and bulbil of wild Chinese yam (Dioscorea polystachya Turcz.) from Wuxue by widely targeted metabolomics

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