›› 2011, Vol. 23 ›› Issue (3): 0-445.

• 作物科学 •    

稻米淀粉性状的QTLs定位及其与淀粉合成相关酶基因的关联性分析

王震1,2, 邵高能1, 魏祥进1, 胡培松1, 唐绍清1, *
  

  1. 1 中国水稻研究所 国家水稻改良中心 水稻生物学国家重点实验室,浙江 杭州 310006;2 杭州师范大学 生命与环境科学学院,浙江 杭州 310036
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2011-05-25 发布日期:2011-05-25

Mapping QTLs for starch traits and its association analysis with starch synthesis-related genes in rice (Oryza sativa L.)

WANG Zhen;SHAO Gao-neng;WEI Xiang-jin;HU Pei-song;TANG Shao-qing;*   

  1. 1 Chinese National Center for Rice Improvement, State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 310006, China; 2 College of Biological and Environmental Sciences, Hangzhou Normal University, Hangzhou 310036, China
  • Received:1900-01-01 Revised:1900-01-01 Online:2011-05-25 Published:2011-05-25

摘要: 利用104个SSR标记对包含178个株系的F7代重组自交系群体(由D50/HB277衍生而来)进行遗传多样性分析,构建了一张覆盖水稻基因组遗传距离1 323 cM,标记间平均遗传距离为11.6 cM的遗传图谱。继而利用28对淀粉合成相关酶基因检测群体多态性,结果筛选到10对有多态的淀粉合成酶基因,整合到遗传图谱上,发现18个调控蒸煮食味品质的QTLs。最后,利用SPSS统计软件计算淀粉合成相关酶基因能解释食味品质表型变异的值,发现计算结果与QTL所得结果大致相同。

关键词: 淀粉合成相关酶基因, 蒸煮品质, 胶稠度, 遗传图谱

Abstract: In this study, a recombinant inbred population consisting of 178 lines, derived from the cross of a japonica (D50) and an indica (HB277) were used to investigate its genetic diversity. We have constructed a genetic linkage map with 104 SSR markers, which covered 1 323 cM genetic distance of rice genome and 11.6 cM average genetic distance between each marker. And then, we have discovered 10 out of 28 starch synthesis-related genes can amplify stable polymorphism among 178 plants, after added them into linkage map, eighteen QTLs were discovered. At last, SPSS statistical software was used to explain the interrelationship between starch synthesis-related genes and cooking and eating quality, and the results were mainly the same as QTL's.

Key words: starch synthesis-related genes, cooking and eating quality, gel consistency, genetic linkage map