›› 2011, Vol. 23 ›› Issue (3): 0-587.

• 食品科学 •    

杭州市粮油类农产品真菌毒素快速检测与安全性评估

张巧艳,王钫,陈健文,王伟,钟钱英,周育*   

  1. 浙江省农业科学院 农产品质量标准研究所,浙江 杭州 310021
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2011-05-25 发布日期:2011-05-25

Mycotoxin rapid detection and safety evaluation for cereal and oil products in Hangzhou markets

ZHANG Qiao-yan;WANG Fang;CHEN Jian-wen;WANG Wei;ZHONG Qian-ying;ZHOU Yu*   

  1. Institute of Quality and Standard for Agricultural Products, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China
  • Received:1900-01-01 Revised:1900-01-01 Online:2011-05-25 Published:2011-05-25

摘要: 在真菌毒素传统检测方法基础上,建立并完善4类真菌毒素免疫亲和净化与荧光法(或HPLC)快速检测技术。应用真菌毒素快速检测技术对杭州市粮油类农产品进行抽样检测并作安全性评估。黄曲霉毒素总量和黄曲霉毒素B1的检测样本数为28个,检出率为25%;赭曲霉毒素A的检测样本数为48个,检出率为22.9%;玉米赤霉烯酮的检测样本数为49个,检出率为42.9%;呕吐毒素的检测样本数为39个,检出率为15.4%。4类真菌毒素的平均检出率约为30%左右。4类真菌毒素安全性评估结果为:黄曲霉毒素和玉米赤霉烯酮超标率最高,呕吐毒素安全性较好。检测结果表明,杭州市粮油类农产品真菌毒素污染广泛,部分真菌毒素超标比较严重,应该进一步加强对谷物及油料类农产品真菌毒素安全性监控工作。

关键词: 真菌毒素, 快速检测, 安全评估

Abstract: Based on conventional assays of mycotoxins, we have established a new rapid detection method for four kinds of mycotoxins using immune purification step and fluorescence(or HPLC) inspection technology. The new method was applied to agricultural products(cereals and oils) in the markets of Hangzhou for mycotoxins inspection and safety evaluation. There were 28 samples for total aflatoxin and aflatoxin B investigation, and the aflatoxin detection rate was about 25.0%; 48 samples were determined for ochratoxin A and the detection rate was 22.9%; 49 samples were determined for zearalenone and the detection rate was 42.9%; 39 samples were determined for deoxynivalenol and the detection rate was 15.4%. The average detection rate of the four kinds of mycotoxin was about 30%. Mycotoxin safety evaluation showed that the MRL(Maximum Residue Limit) exceeding ratio of aflatoxin and zearalenone were higher than the other two mycotoxins, and the safety level of deoxynivalenol was higher than the other three mycotoxins in Hangzhou agricultural product markets. Overall, there were widespread contaminations of cereal and oil agricultural products by mycotoxins in Hangzhou and some types of mycotoxins exceeded far beyond the MRL. Much effort should be placed to the regulation of mycotoxins safety of the cereal and oil products.

Key words: mycotoxin, rapid inspection, safety evaluation