关键词: 泥鳅, 嗜水气单胞菌, 右旋糖酐铁, GSTs

Abstract:

Mud loach is widly distributed in East\|Asia. Previous reports showed that glutathione\|S\|transferase (GSTs) was one key element in the detoxication procedure of immune system. The aim of this study was to investigate the influences of different concentrations of Aeromonas hydrophila and iron dextran on glutathione\|S\|transferase in mud loach. The liver was extracted and homogenated to obtain the GSTs in different time after injection. The activities of GSTs were analyzed and the results showed that different concentrations of Aeromonas hydrophila induced the expression of GSTs in livers of loaches. The group treated with 21×109cfu·mL-1 had the strongest ability to induce the activity of GSTs. However, the group treated with 21×107 cfu·mL-1 and 21×105 cfu·mL-1 showed a smaller induction on activity of GSTs than 21×109 cfu·mL-1 group. The activity of GSTs reached the peak several hours after injection and then dropped to the normal level, and the GSTs activities of treatment groups were significantly higher than the control. After injection of iron dextran, we found that different concentrations of iron dextran showed different effects on GSTs activities. The GSTs activities was the highest when treated with 10 μg·g-1 iron dextran, and reached the peak at 1 d after injection and then dropped to the normal level during the following days. However, the GSTs activities was inhibited when treated with 25 μg·g-1 and 50 μg·g-1 iron dextran, which was the strongest at 4 d after injection with iron dextran and then increased to the normal level．

Key words: mud loach, Aeromonas hydrophila, iron dextran, GSTs