›› 2012, Vol. 24 ›› Issue (6): 0-1063.

• 论文 •    

双孢蘑菇基质中隐杆线虫Caenorhabditis sp-CNM生防潜力研究

李星月, 刘奇志*,杨道伟   

  1. 中国农业大学 农学与生物技术学院,昆虫与线虫学实验室,北京 100193
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2012-11-25 发布日期:2012-11-25

Biocontrol potential of Caenorhabditis sp CNM strain discovered from the compost of Agaricus bisporus

LI Xingyue;LIU Qizhi*;YANG Daowei   

  1. Laboratory of Entomology and Nematology,College of Agriculture and Biotechnology, China Agricultural University, Beijing 100193, China
  • Received:1900-01-01 Revised:1900-01-01 Online:2012-11-25 Published:2012-11-25

摘要: 从双孢菇基质中诱集得到的一种隐杆属线虫(Caenorhabdtis sp CNM)具有4天快速致死大蜡螟(Galleria mellonella),为探讨其生防潜力,对其进行了侵染力、抗逆能力及运动能力的研究。结果显示:小杆线虫CNM对大蜡螟的LD50(48 h)为 46.6 IJs·larva-1,LC50(48 h)为 53.8 IJs·cm-2。CNM线虫在42℃,40℃和38℃水浴下全部个体死亡所需时间分别为45 min,20 h和55 h。CNM线虫在RH 50.5%、温度25℃条件下存放12 h,虽有46.0%个体存活,但侵染力下降43.3%。有51.7%的CNM线虫在30℃环境下经过24 h可爬行3.6 cm寻找大蜡螟寄主体液。由此认为,CNM线虫可以作为有效的生物防治制剂,用于害虫的防治。

关键词: 双孢蘑菇, 隐杆线虫, 生物防治, 侵染力, 抗逆能力

Abstract: One species of insect nematode (Caenorhabditis sp CNM), which can cause Galleria mellonella death within 4 days, was discovered in the compost of Agaricus bisporus through the Galleria bait method In order to discover the biologicalcontrol potential of CNM in the field application, we investigated its biology characteristics, especially the infectivity and antistress resistance The result of its infectivity to G mellonella showed that the larger of the dosage, the better of its infectivity LD50 of CNM to G mellonella was 46.6 IJs per larva, and the LC50 is 53.8 IJs·cm-2 at 48 h After 45 min, 20 h and 55 h, all of the nematodes died with water bathing of 42℃, 40℃ and 38℃ Therere still 46.0% of the nematodes alive after 12 h under 25℃, 50.5 % RH, and the infectivity decrease was 43.3% Besides, there were re 57.1% of nematode that can move for as far as 3.6 cm under 30℃ So Caenorhabditis sp CNM strain has the biocontrol potential with good infectivity, heat resistance and desiccation.

Key words: Agaricus bisporus, Caenorhabditis, biocontrol, infectivity, antistress resistance