关键词: 桑黄, RNA提取, Trizol法, RT\|PCR

Abstract: In order to obtain an ideal total RNA extraction method of Phellinus baumii， total RNA of Phellinus baumii was extracted with 3 methods including Trizol method， CTAB method and RNA prep pure Plant Kit method. The results showed that all these methods can extract total RNA from Phellinus baumii， however， the Trizol method was the best and had high integrity and purity. The band of 28S and 18S was clear. The value of A260/A280 was 196 and the RNA yield was 60464 μg·g-1. Thus， the RNA extracted by Trizol method could be used for RT\|PCR， gene cloning and gene expression and so on.

Key words: Phellinus baumii Pilat, RNA extraction, Trizol method, RT\|PCR