›› 2014, Vol. 26 ›› Issue (2): 0-290296.

• 动物科学 •    

Asia1型口蹄疫病毒基因组的测序分析

王琳萱1,陈思敏1,张印红1,*,孙东杰2   

  1. 1兰州大学 公共卫生学院营养与食品卫生研究所,甘肃 兰州 730000;2中国农业科学院 兰州兽医研究所/家畜疫病病原生物学国家重点实验室,甘肃 兰州 730046
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2014-03-25 发布日期:2014-07-09

Genome sequencing and analysis in Asia1 serotype strain of footandmouth disease virus

WANG Linxuan;CHEN Simin;ZHANG Yinhong;*;SUN Dongjie   

  1. 1Nutrition and Food Hygiene Research Institute, College of Public Hygiene, Lanzhou University, Lanzhou 730000, China; 2 Veterinary Research Institute, Chinese Academy of Agricultural Sciences/State Key Laboratory of Veterinary Etiological Biology, Lanzhou 730046, China
  • Received:1900-01-01 Revised:1900-01-01 Online:2014-03-25 Published:2014-07-09

摘要: 对中国口蹄疫病毒Asia1/JS/2005毒株的全基因组进行了分段扩增并测序。结果表明,该毒株的基因组全长为8 174 nt,其中 5’UTR 为1 092 nt;ORF为6 990 nt;3’UTR为92 nt。利用DNAstar软件对该毒株和Asia1型其他24毒株基因组序列进行同源性分析表明,该毒株的非结构蛋白和5’UTR比较保守。研究还发现了可能与生物学功能相关的新的保守和变异区域。系统树分析表明Asia1/JS/2005, Asia1/1/YZ/2006,Asia1/WHN/2006,Asia1/HN/2006和Asia1/YS/2005属于同一分支,表明这些毒株间有着紧密的遗传关系,其中与其亲缘关系最为密切的是Asia1/YS/2005。

关键词: 口蹄疫病毒, Asia1/JS/2005, 基因组

Abstract: In this study, the whole genome of footandmouth disease virus (FMDV) vaccine strain Asia1/JS/2005 in China was amplified and sequenced. The results showed that the complete genome is 8 174 nt, including a 1 092 nt 5’ untranslated region (UTR), a 6 990 nt open reading frame and a 92 nt 3’UTR. By using the DNAstar software, homology analysis of genome sequences of strain Asia1/JS/2005 and other 24 Asia1 strains were performed. The results indicated that nonstructural proteins and 5’UTR were more conserved. The data revealed novel highly conserved and variable genomic regions, which was likely associated with biological function. Phylogenetic tree revealed that Asia1/JS/2005 is closely linked with Asia1/1/YZ/2006, Asia1/WHN/2006, Asia1/HN/2006 and Asia1/YS/2005, which suggested that they were genetically associated and Asia1/YS/05 was the most closely related strain with Asia1/JS/2005.

Key words: footandmouth disease virus, Asia1/JS/2005, genome