关键词: 丁香, 花粉, 超低温保存

Abstract: Lilac (Syringa L.) are important garden plants with abundant species in China. The research on the pollen preservation of lilac is of significant meanings for germplasm conservation and hybridization breeding. The germination of 12 species of fresh lilac pollen preserved in room temperature (25℃)， refrigerator (4℃) and liquid nitrogen (LN2，-196℃) were determinate respectively after different storage time. The results demonstrated that the 11 species of lilac pollen preserved at 25℃ can be preserved for 7 d， and 5 species of them can survive after 30 d. Whereas， 12 species of lilac pollen preserved at 4℃ can be preserved for as long as 30 d， but only S. villosaand and S. oblata were alive after 180 d， and the germination rate (219% and 150%， respectively) was significantly lower than the fresh pollen. In contrast， under-196℃， 11 species of lilac pollen can be preserved for 180 d， and there were no significant difference from the fresh pollen in germination rate．

Key words: Syringa L., pollen, cryopreservation